We narrowed to 8,600 results for: Set

1. IRE-miR21-SunTag-TREAT

   Plasmid

   #196932

   Purpose

   SunTag cassette was added in the coding region in the miR21-IRE-TREAT reporter.

   Depositor

   Jeffrey Chao

   Article

   Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

   Insert

   Renilla luciferase
   

   Expression

   Bacterial and Mammalian

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)MBII85

   Plasmid

   #67643

   Purpose

   MBII85 snoRNA expression cassett, contains snoRNA in natural intron surrounded by natural exons.

   Depositor

   Stefan Stamm

   Article

   Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

   Insert

   MBII85 snoRNA
   

   Expression

   Mammalian

   Available Since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFLIP38

   Plasmid

   #65616

   Purpose

   FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

   Depositor

   Wolf Frommer

   Article

   Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

   Type

   Empty backbone

   Tags

   6His-edCit-attR1 and attR2-edCer-cMyc

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKDC.015

   Plasmid

   #184992

   Purpose

   Express -Eco2 RT and ncRNA in human cells

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco2: RT and ncRNA(wt), a1/a2 length: 13
   

   Expression

   Mammalian

   Mutation

   Human codon optimized RT

   Promoter

   TetOn-3g

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. GGE252

   Plasmid

   #120791

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   InsDown_GSY-NotI
   

   Use

   Cloning/assembly plasmid

   Available Since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. GGE253

   Plasmid

   #120790

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   InsUp_GSY-NotI
   

   Use

   Cloning/assembly plasmid

   Available Since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCfB8383

   Plasmid

   #126917

   Purpose

   Plasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of tHMG1

   Depositor

   Irina Borodina

   Article

   Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

   Insert

   tHMG1
   

   Expression

   Yeast

   Available Since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFLIP43

   Plasmid

   #65618

   Purpose

   FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

   Depositor

   Wolf Frommer

   Article

   Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

   Type

   Empty backbone

   Tags

   6His-edAFPt9-attR1 and attR2-edCer-cMyc

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJUNB-donor

   Plasmid

   #112313

   Purpose

   CRISPR donor plasmid to tag human transcription factor JUNB with GFP

   Depositor

   Kevin White

   Insert

   JUNB homology arms flanking EGFP-IRES-Neo cassette (JUNB Human)
   

   Use

   CRISPR

   Mutation

   hg38:19:12792992:C>G, hg38:19:12793018:T>G,…

   Available Since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHEY1-donor

    Plasmid

    #112297

    Purpose

    CRISPR donor plasmid to tag human transcription factor HEY1 with GFP

    Depositor

    Kevin White

    Insert

    HEY1 homology arms flanking EGFP-IRES-Neo cassette (HEY1 Human)
    

    Use

    CRISPR

    Mutation

    hg38:8:79765311:G>A

    Available Since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCas9–mKate2ps–EgRNA

    Plasmid

    #62716

    Purpose

    Empty; no U6 gRNA cassette

    Depositor

    Leonidas Bleris

    Article

    Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

    Insert

    Cas9–mKate2ps
    

    Expression

    Mammalian

    Available Since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

12. KaiC CI cat-/EE (E77Q; E78Q; S431E; T432E)

    Plasmid

    #41888

    Depositor

    Michael Rust

    Article

    Phong et al Proc Natl Acad Sci U S A. 2013 Jan 15;11

    Insert

    Kai C CI cat-/EE
    

    Expression

    Bacterial

    Mutation

    changed amin acids: 77,78,431 and 432 to glutamate

    Promoter

    t7

    Available Since

    March 19, 2013

    Availability

    Academic Institutions and Nonprofits only

13. vector-dendra2 PCDNA

    Plasmid

    #121134

    Purpose

    mammalian cell expression

    Depositor

    Qing Deng

    Article

    Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

    Insert

    empty cassete for contol of miR expression
    

    Expression

    Bacterial

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. 5VC8 (WHSC1)

    Plasmid

    #101262

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    WHSC1 (NSD2 Human)
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 2, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. GGE209

    Plasmid

    #120793

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    InsUp-Mfe
    

    Use

    Cloning/assembly plasmid

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCfB8622

    Plasmid

    #126912

    Purpose

    Plasmid containing gRNA expression cassette targeting ADE2 in Saccharomyces cerevisiae

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    ADE2 gRNA
    

    Use

    CRISPR

    Available Since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFLIP37

    Plasmid

    #65505

    Purpose

    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Tags

    6His-Cit-attR1 and attR2-Cer-cMyc

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOVC1_L4(EAAAK)3

    Plasmid

    #131211

    Purpose

    Cloning vector containing A-B-C casette with linker L4 in site B

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    (EAAAK)3 linker
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. YN2_1_LT84_RPL13A

    Plasmid

    #177712

    Purpose

    Expresses Cas9 and sgRNA cassette for targeting RPL13A in yeast Saccharomyces cerevisiae

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pICSL70005

    Plasmid

    #50335

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    bialaphos resistance cassette (Nos promoter (A. tumefaciens) + phosphinothricin acetyl transferase (S. hygroscopicus) + Nos term
    

    Use

    Plant expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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