We narrowed to 12,018 results for: cel.2;

1. pcDNA3.1_mGFP-FGFR3-G380R

   Plasmid

   #191755

   Purpose

   Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-G380R mutation (associated with ACH; target mutation: c.1138G>A; silent mutation added on purpose: c.1131C>T)

   Depositor

   Irene Tiemann-Boege

   Article

   Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

   Insert

   mGFP-FGFR3IIIc
   

   Use

   Tags

   mGFP

   Expression

   Mammalian

   Mutation

   c.1138G>A (G380R) + c.1131C>T (silent mutat…

   Promoter

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pet21a-aGFPnb-enhancer-cys-linker-YbbR-(PAS)5-H6

   Plasmid

   #192788

   Purpose

   aGFP nanobody enhancer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labeling

   Depositor

   Jacob Piehler

   Article

   Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

   Insert

   antiGFP nanobody enhancer
   

   Use

   Tags

   H6 and ybbR

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEMS2260

   Plasmid

   #158423

   Purpose

   AAV plasmid with Ple331 (PAX6 MiniPromoter) driving expression of EmGFP. Contains WPRE.

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

   Insert

   Ple331 (PAX6 Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Ple331

   Available since

   Feb. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRP[2CRISPR]-mCherry/Hygro-hCas9-U6>{mdx left}-U6>{mdx right}

   Plasmid

   #184381

   Purpose

   This plasmid contains hCas9 and two gRNAs that can excise the genomic area around the mouse mdx mutation in dystrophin's exon 23

   Depositor

   Ori Bar-Nur

   Article

   Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

   Insert

   Cas9, two gRNAs targeting dystrophin
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. b2AR->M71-IRES-tauGFP-ACNF TV

   Plasmid

   #105198

   Purpose

   targeting vector to generate a replacement of CDS of M71 with CDS of mouse beta2-adrenergic receptor with expression of tauGFP.

   Depositor

   Peter Mombaerts

   Article

   Omura et al Mol Cell Neurosci. 2014 Jan;58:1-10. doi

   Insert

   b2AR->M71-IRES-tauGFP-ACNF (Adrb2, Olfr151 Mouse)
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRC/RSV-rat Agt

   Plasmid

   #122104

   Purpose

   Expression of angiotensinogen

   Depositor

   John Chan

   Article

   Zhang et al J Am Soc Nephrol. 2002 Feb;13(2):302-12.

   Insert

   angiotensinogen (Agt Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   RSV promoter

   Available since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. HOXB6 (human) FLAG pCMV

   Plasmid

   #8815

   Depositor

   Corey Largman

   Article

   Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

   Insert

   HOXB6 (HOXB6 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   contains improved ribosomal binding site and FLAG…

   Promoter

   Available since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pPB9P3

   Plasmid

   #210034

   Purpose

   Contains cloning site for PE3/3b prime editing gRNA constructs, inducible prime editor 2, rtTA-T2A-puro

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   prime editor 2
   rtta-T2A-puro
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α and TRE3G

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3-SREBP-1 (A317-318)

   Plasmid

   #204193

   Purpose

   Mammalian expression of SREBP-1 (isoform 1)

   Depositor

   Allan Weissman

   Article

   Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

   Insert

   SREBP-1 (SREBF1 Human)
   

   Use

   Tags

   E-tag and T7x2

   Expression

   Mammalian

   Mutation

   Gly to Ala 317, 318

   Promoter

   Available since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA3-SREBP-1 (A317-318; A341)

    Plasmid

    #204194

    Purpose

    Mammalian expression of SREBP-1 (Isoform 1)

    Depositor

    Allan Weissman

    Article

    Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

    Insert

    SREBP-1 (SREBF1 Human)
    

    Use

    Tags

    E-tag and T7x2

    Expression

    Mammalian

    Mutation

    Gly to Ala 317,318,341

    Promoter

    Available since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA3-SREBP-1 (A341; A1171)

    Plasmid

    #204197

    Purpose

    Mammalian expression of SREBP-1 (isoform 1)

    Depositor

    Allan Weissman

    Article

    Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

    Insert

    SREBP-1a (SREBF1 Human)
    

    Use

    Tags

    E-tag and T7x2

    Expression

    Mammalian

    Mutation

    Gly to Ala 341, 1171

    Promoter

    Available since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. B2M-SDMutation-gRNA

    Plasmid

    #215547

    Purpose

    gRNA targeting B2M to introduce splice donor mutation on the first intron of the locus

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Insert

    B2M (B2M Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. INS-3'gRNA

    Plasmid

    #210468

    Purpose

    gRNA targeting INS 3' terminal for CRISPR-Cas9-mediated knock-in

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Insert

    INS (INS Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. tetO-ZFPM2

    Plasmid

    #170707

    Purpose

    doxycycline-inducible overexpression of ZFPM2

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    ZFPM2 (ZFPM2 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    3 nonsynonymous changes: 353C>T (Ser>Phe)…

    Promoter

    TRE promoter, Tet-ON

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pROS_phleo-Tps1/Gsy2

    Plasmid

    #196612

    Purpose

    Encoding guide RNAs for the knock out of TPS1 and GSY2 genes

    Depositor

    Matthias Heinemann

    Article

    Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

    Insert

    TPS1, GSY2 (TPS1, GSY2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-EF1α-Flex/3'USS-ChRWR/Venus(ATG mut)

    Plasmid

    #197891

    Purpose

    Can be used to generate AAV virus that will express ChRWR/Venus in the presence of Cre and the leakage expression is significantly reduced without Cre

    Depositor

    Kazuto Kobayashi

    Article

    Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

    Insert

    ChRWR/Venus
    

    Use

    AAV

    Tags

    Expression

    Mutation

    N/A

    Promoter

    EF1a

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUGP1.1

    Plasmid

    #196615

    Purpose

    Plasmid used for the C-terminal tagging of Ugp1 with mCherry and the auxin-inducible degron in yeast

    Depositor

    Matthias Heinemann

    Article

    Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

    Insert

    UGP1::mCherry-AID(71-114)-NatMX (UGP1 Budding Yeast)
    

    Use

    Tags

    AID(71-114) and mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Native promoter

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1_mGFP-FGFR3-Y373C

    Plasmid

    #191754

    Purpose

    Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-Y373C mutation (associated with TD1) (target mutation: c.1118A>G ; silent mutation added on purpose: c.1110C>A)

    Depositor

    Irene Tiemann-Boege

    Article

    Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

    Insert

    mGFP-FGFR3IIIc
    

    Use

    Tags

    mGFP

    Expression

    Mammalian

    Mutation

    c.1118A>G, p.Y373C + c.1110C>A (silent muta…

    Promoter

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. ABHD2-3'UTR-Del-1

    Plasmid

    #190095

    Purpose

    examine the activity of ABHD2 3'UTR

    Depositor

    Bowen Liu

    Article

    Liu et al Commun Biol. 2022 Aug 13;5(1):816. doi:

    Insert

    ABHD2 3' UTR delete-1 (ABHD2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    deleted the base pairs 6704-6709 refer to NM_0070…

    Promoter

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR207-MUM2sp-Citrine-ADPG2

    Plasmid

    #170725

    Purpose

    Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the Citrine and the coding sequence of the HG degrading enzyme ADPG2 (At2g41850.1)

    Depositor

    George Haughn

    Article

    McGee et al Plant Cell Physiol. 2021 Jun 1. pii: 629

    Insert

    ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2 (PGAZAT Mustard Weed)
    

    Use

    Gateway donor vector / entry clone

    Tags

    Citrine tag (714bp)

    Expression

    Mutation

    Promoter

    Available since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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