We narrowed to 19,150 results for: Tec

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pOCC61

Plasmid

#118877

Purpose

shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP and C-terminal HIS6 tag, cleavable with 3C

Depositor

Marc Bickle, PEPC MPI CBG

Article

Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

Insert

NcoI-NotI-ccdB-AscI-mGFP-3C-HIS6-stop-HindIII cassette

Tags

mGFP, HIS6, cleavable with 3C protease

Expression

Insect

Promoter

polH

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pTS2394-Tier1-PhCMV-3xNLS_mTagBFP2

Plasmid

#169531

Purpose

Tier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 with nuclear localization (PhCMV-3xNLS-mTagBFP2-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven nucleus-localized monomeric Tag blue fluorescent protein 2

Expression

Mammalian

Promoter

PhCMV

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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TAL2302

Plasmid

#36731

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

BRCA2-TALEN_Left (Brca2 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2303

Plasmid

#36732

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

BRCA2-TALEN_Right (Brca2 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
p2CT-His-MBP-Lbu_C2c2_R1048A_H1053A

Plasmid

#83484

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 2 inactive mutant).

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R1048A_H1053A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R1048A, H1053A

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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AA345

Plasmid

#216031

Purpose

Fragmid fragment: (Cas protein) Cas13d

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Cas13d_v1.1 [Dj]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pspCas9-ABE-3'UTR-sgRNA-ST2-com vector

Plasmid

#136270

Purpose

Expresses ABE and ST2-com modified sgRNA scaffold

Depositor

Baisong Lu

Article

Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

Insert

ABE and sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Nov. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pRDA_834

Plasmid

#216087

Purpose

Cas9 [Sp] knockout targeting CD59, positive control

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD59 guide

Use

CRISPR and Lentiviral; Positive controls

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pTS2396-Tier1-PhCMV-4xCOX8_Ypet

Plasmid

#169534

Purpose

Tier-1 vector encoding PhCMV-driven yellow fluorescent protein YPet localized to the mitochondria (PhCMV-4xCOX8-YPet-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven miochondria-localized yellow fluorescent protein Ypet

Expression

Mammalian

Promoter

PhCMV

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pPSP6+t

Plasmid

#48149

Purpose

Shuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage SP6

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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AYBEv0.1

Plasmid

#193966

Purpose

A wildtype adenine transversion base editor (AYBEv0.1, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoter

Depositor

Huawei Tong

Article

Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

ecTadA8e-nSpCas9(D10A)-MPG(wt)

Expression

Mammalian

Promoter

hU6, EF1a, CBh

Available Since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 TGFB1

Plasmid

#67080

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TGFB1 (TGFB1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pGWB524

Plasmid

#74866

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with GST (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB529

Plasmid

#74871

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with TAP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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EK0325 SFFV-mCherry-SG(EGFP.s2)SG-Cre (FLP-IN)

Plasmid

#191160

Purpose

Expression of a sensor for EGFP CDS in mammalian cells; mCherry marker, Cre output

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:EGFP.s2:Cre

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pUC57mini ZM249M P90A _env eGFP

Plasmid

#132939

Purpose

pUC57mini ZM249M _env eGFP containing P90A mutant capsid sequence

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

HIV-1ZM249M provirus (P90A) with env deletion, GFP

Use

Lentiviral

Mutation

P90A

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCfB3035(X-4 MarkerFree)

Plasmid

#73273

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-4 (Chr X: 236336..237310)

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pPMVAd36

Plasmid

#98302

Purpose

Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or CUP1 promoter.

Depositor

Claudia Vickers

Article

Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

Insert

PCUP1>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1

Expression

Yeast

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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p045

Plasmid

#70057

Purpose

Co-expresses Syt1-SNARE37aa-linker complex with pDC184

Depositor

Axel Brunger

Article

Zhou et al Nature. 2015 Sep 3;525(7567):62-7. doi:

Inserts

C2AB-linker-SNAP-25_C
SNAP-25_N

Tags

No tags

Expression

Bacterial

Promoter

T7

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pGWB443

Plasmid

#74829

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with eCFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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