We narrowed to 9,414 results for: pho;

1. pmirGLO-Smad4-1719

   Plasmid

   #49383

   Purpose

   Insert is a fragment of the Smad4 3'-UTR that contains miR-17 and miR-19 binding sites. To be used for 3'UTR assay.

   Depositor

   Martine Roussel

   Article

   Murphy et al Cancer Res. 2013 Oct 21.

   Insert

   Smad family member 4 (SMAD4 Human, Mouse)
   

   Use

   Luciferase

   Promoter

   Human phosphoglycerate kinase (PGK) promoter

   Available Since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMH-HT_ygiF-CHAD

   Plasmid

   #127793

   Purpose

   Expresses the CHAD domain of ygiF in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   ygiF-CHAD (ygiF Escherichia coli)
   

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   N-terminal TTM domain of ygiF (residues 201-433)

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CAG-Flex-mGFP-APEX2

   Plasmid

   #171936

   Purpose

   Reporter for correlated light and electron microscopy.

   Depositor

   David Berson

   Article

   Stedehouder et al Elife. 2019 Nov 19;8. pii: 48615. doi: 1

   Insert

   mGFP-APEX2
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS5

   Plasmid

   #55750

   Purpose

   Produces one half (beta) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.

   Depositor

   Matthew Bennett

   Article

   Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

   Insert

   KanR
   

   Expression

   Bacterial

   Mutation

   Residues 59-264 of KanR

   Promoter

   T7-lac

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. HOXC8-pSG5

   Plasmid

   #21001

   Depositor

   Corey Largman

   Article

   Lawrence et al Cell Growth Differ. 1993 Aug . 4(8):665-

   Insert

   HOXC8 (HOXC8 Human)
   

   Expression

   Mammalian

   Mutation

   missing first 24 N-terminal amino acids of coding…

   Available Since

   Aug. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

6. pENTR-p27(kip1)

   Plasmid

   #50026

   Purpose

   pENTR vector for human cyclin-dependent kinase inhibitor 1B

   Depositor

   Jamie Davies

   Article

   Cachat et al J Biol Eng. 2014 Nov 19;8(1):26. eCollec

   Insert

   p27(kip1) (CDKN1B Human)
   

   Use

   Entry vector

   Available Since

   Dec. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pJet-CMV-CD5HA2-bglpA

   Plasmid

   #96962

   Purpose

   Expression plasmid for HA-tag embedded into human CD52 with short polyA

   Depositor

   Dmitriy Mazurov

   Article

   Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

   Insert

   CMV-CD5HA2-bglpA
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pERB257

   Plasmid

   #67761

   Purpose

   Expresses KLC1-mCherry-eDHFR

   Depositor

   Michael Lampson

   Article

   Ballister et al Curr Biol. 2015 May 18;25(10):R407-8. do

   Insert

   KLC1-mCherry-eDHFR (Klc1 Rat)
   

   Tags

   eDHFR and mCherry

   Expression

   Mammalian

   Mutation

   contains Klc1 amino acids 1-175

   Promoter

   CAG

   Available Since

   Jan. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMTH PKC beta II

   Plasmid

   #8410

   Depositor

   Frederic Mushinski

   Article

   Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

   Insert

   Rat Protein Kinase C beta II (Prkcb Rat)
   

   Expression

   Mammalian

   Available Since

   April 28, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pEPQD1CB0109

    Plasmid

    #177042

    Purpose

    MoClo Level 1, position 2, transcriptional unit for transient expression of geranyl pyrophosphate synthase (PaGPPS1) from Picea abies driven by 35S promoter; contains a chloroplast transit peptide

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    geranyl pyrophosphate synthase (PaGPPS1) from Picea abies
    

    Use

    Synthetic Biology

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJR2953

    Plasmid

    #122549

    Purpose

    Expression of a C. elegan codon optimized florescent protein (CemCardinal2) under the intestinal specific promoter nhx-2

    Depositor

    Gary Silverman

    Article

    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

    Insert

    Pnhx-2_CemCardinal2
    

    Expression

    Bacterial

    Promoter

    Pnhx-2

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a Gx-NL2

    Plasmid

    #169107

    Purpose

    Expresses protein G fused to 2 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

    Depositor

    Maarten Merkx

    Article

    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

    Insert

    protein G - NanoLuc - NanoLuc
    

    Tags

    his-tag and strep-tag

    Expression

    Bacterial

    Mutation

    Ala 24 stop

    Promoter

    T7

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. 813 pSG5L HA PTEN 1-274

    Plasmid

    #10740

    Depositor

    William Sellers

    Article

    Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

    Insert

    PTEN (PTEN Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Contains PTEN amino acids 1-274.

    Available Since

    Sept. 27, 2005

    Availability

    Academic Institutions and Nonprofits only

14. pEGFP_C1 I3

    Plasmid

    #61397

    Purpose

    Expresses GFP-tagged Inhibitor-3 in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

    Insert

    Inhibitor-3 (PPP1R11 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

15. SFG.CNa22_opt.IRES.eGFP

    Plasmid

    #22491

    Depositor

    Martin Pule

    Article

    Brewin et al Blood. 2009 Nov 26;114(23):4792-803. doi

    Insert

    Codon optimised Calcineurin A mutant 22 (PPP3CA Human)
    

    Use

    Retroviral

    Mutation

    V314R; Y341F

    Available Since

    Dec. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEPQD1CB0108

    Plasmid

    #177041

    Purpose

    MoClo Level 1, position 2, transcriptional unit for transient expression of geranyl pyrophosphate synthase (PaGPPS1) from Picea abies driven by 35S promoter

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    geranyl pyrophosphate synthase (PaGPPS1) from Picea abies
    

    Use

    Synthetic Biology

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. miR-144 Trap

    Plasmid

    #60934

    Purpose

    to knockdown endogenous miR144 levels; lentiviral vector containing 8x copies of complementary miR-144-binding sites cloned 3' to GFP under the control of a biPGK promoter

    Depositor

    Curt Civin

    Article

    Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

    Insert

    miR-144 Trap
    

    Use

    Lentiviral

    Tags

    GFP

    Promoter

    bidirectional phosphoglycerate kinase promoter (b…

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pRSM32.2

    Plasmid

    #101635

    Purpose

    Encodes the fluorescent protein Rosmarinus under a CMV promoter.

    Depositor

    Thomas Hughes

    Article

    Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

    Insert

    Rosmarinus
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNB784

    Plasmid

    #60163

    Purpose

    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by SIC1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc-yEVenus
    

    Expression

    Yeast

    Mutation

    FLuc has A4V & S504G (no functional effect)

    Promoter

    SIC1pr

    Available Since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pVH615

    Plasmid

    #169604

    Purpose

    Tier-2 vector encoding PCREm4-driven Nluc and PmPGK1-driven Fluc expression (A1-pA::PCREm4-Nluc-pA::PmPGK1-Fluc-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette
    

    Expression

    Mammalian

    Promoter

    PCREm4 / PPGK

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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