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pXPR_BRD051-sgMLH1-2

Plasmid

#125777

Purpose

constitutive expression of a guide RNA targeting human MLH1 (CRISPR cutting control) and S. pyogenes Cas9

Depositor

Francisca Vazquez

Publication

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgMLH1-2 (MLH1 Human)

Use

CRISPR

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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HA-UBE2E1 C131A in pcDNA3.1

Plasmid

#118214

Purpose

Expresses HA-UBE2E1 C131A catalytic mutant in mammalian cells

Depositor

Cynthia Wolberger

Publication

Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

Insert

UBE2E1 (UBE2E1 Human)

Tags

HA

Expression

Mammalian

Mutation

changed cysteine 131 to alanine

Available since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shCELF4-4405

Plasmid

#115466

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Publication

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shCELF4-4405 (CELF4 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shCELF4-4404

Plasmid

#115465

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Publication

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shCELF4-4404 (CELF4 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pETDuet RagA-RagC(S75N)

Plasmid

#99645

Purpose

Bacterial expression of RagA-RagC(S75N)

Depositor

David Sabatini, Kuang Shen

Publication

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Tags

His tag

Expression

Bacterial

Mutation

S75N

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pINDUCER20 MEF2C S222A

Plasmid

#89718

Purpose

Lentiviral gene expression vector for doxycycline inducible MEF2C-S222A expression

Depositor

Alex Kentsis

Publication

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MEF2C (MEF2C Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed serine 222 to alanine

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR221-CCL5.Pro

Plasmid

#79492

Purpose

MultiSite Gateway entry clones, first fragment, (attL1, attR5) CCL5/RANTES promoter

Depositor

Peter Jon Nelson

Publication

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

CCL5 Promoter (CCL5 Human, Synthetic)

Use

Gateway Cloning

Promoter

CCL5 Promoter

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-alpha4 wt (Hygro)

Plasmid

#80017

Purpose

Mammalian expression of human integrin alpha4, Hygro selection

Depositor

Chinten James Lim

Publication

Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

integrin alpha4 (ITGA4 Human)

Expression

Mammalian

Promoter

CMV

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only
myr-FLAG-MEK5-pcw107

Plasmid

#64620

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Publication

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

MAP2K5 (MAP2K5 Human)

Use

Lentiviral

Tags

Flag and myristoylated

Promoter

PGK

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only
sgRNA2_IL1RN

Plasmid

#64142

Purpose

Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Publication

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA2_IL1RN (IL1RN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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sgRNA4_IL1RN

Plasmid

#64152

Purpose

Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Publication

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA4_IL1RN (IL1RN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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mEos2-Calreticulin-N-10

Plasmid

#57353

Purpose

Localization: Endoplasmic Reticulum, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Publication

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Calreticulin (CALR Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
PBRPyCAG-LoxP-ERAS-STOPcassette-Loxp-DsRed-IRES-PURO

Plasmid

#52419

Purpose

Constitutive mammalian expression of ERAS. Upon CRE mediated deletion of ERAS insert, dsRED expression markers is truned on due to concomittant removal of stop cassette.

Depositor

Jacob Hanna

Publication

Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

Insert

ERAS (ERAS Human)

Promoter

CAGGS

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pBEG R2-iNeo-L3 #BGV207

Plasmid

#48986

Purpose

Supplies the selection marker (neomycin resistance) and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

Depositor

David Dankort

Publication

Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

Insert

neomycin resistance

Use

Gateway Cloning

Tags

IRES (weak)

Promoter

NA

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
phpRL-BCL2-FL-pA

Plasmid

#42595

Depositor

Richard Lloyd

Publication

Sherrill et al J Biol Chem. 2004 Jul 9;279(28):29066-74

Insert

hairpin - renilla luciferase - BCL2 5'UTR - firefly luciferase

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pVRa42_4454

Plasmid

#49703

Depositor

Christopher Voigt

Publication

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF42_4454

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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PU.1-W215G LZRS

Plasmid

#44635

Depositor

Ellen Rothenberg

Publication

Anderson et al Immunity. 2002 Feb;16(2):285-96.

Insert

PU.1 W215G (Spi1 Mouse)

Use

Retroviral

Tags

EGFP and IRES

Expression

Mammalian

Mutation

215 mutation (W to G)

Available since

Oct. 24, 2013

Availability

Academic Institutions and Nonprofits only
SFG.CNa12_opt.IRES.eGFP

Plasmid

#22490

Depositor

Martin Pule

Publication

Brewin et al Blood. 2009 Nov 26;114(23):4792-803. doi

Insert

Codon optimised Calcineurin A mutant 12 (PPP3CA Human)

Use

Retroviral

Mutation

T351E; L354A

Available since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

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HOXD12 (murine) HIS-tag pET

Plasmid

#8553

Depositor

Corey Largman

Publication

Shen et al Mol Cell Biol. 1997 Nov;17(11):6448-58

Insert

HOXD12 (Hoxd12 Mouse)

Tags

His and T7

Expression

Bacterial

Mutation

Fusion sequence is: ggatccATG

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pTBX1-αRabbit-12xSunTag-sfGFP

Plasmid

#254519

Purpose

For use with CoCUT&Tag method, binds rabbit primary antibody and recruits αSunTag-Tn5

Depositor

Derek Janssens

Publication

Sun et al bioRxiv 2026.04.27.721191

Insert

anti-Rabbit::12xSunTag::sfGFP

Tags

Chitin Binding Domain and Mxe GyrA intein

Expression

Bacterial

Available since

May 12, 2026

Availability

Academic Institutions and Nonprofits only

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