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pUCHR-inLuc

Plasmid

#58955

Purpose

HIV-1 transfer vector, encodes firefly luciferase gene interrupted by a gamma-globin intron. The reporter gene is silent in the transfected cells and expressed in the infected cells.

Depositor

David Derse, Dmitriy Mazurov

Article

Mazurov et al PLoS Pathog. 2010 Feb 26;6(2):e1000788.

Insert

inLuc

Use

Lentiviral

Promoter

CMV

Available Since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV10-hTti1

Plasmid

#30213

Depositor

Noboru Mizushima

Article

Kaizuka et al J Biol Chem. 2010 Jun 25. 285(26):20109-

Insert

Tel2 interacting protein 1 (TTI1 Human)

Tags

FLAG

Expression

Mammalian

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
pCMU-TGN41r

Plasmid

#61176

Purpose

Fluorescent reporter for trans-Golgi network mCherry-MtSYP41 expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtSYP41

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV_hDlx_DIO_ChR2_mCherry

Plasmid

#224459

Purpose

An AAV vector expressing ChR2-mCherry that is dependent on both Cre and the hDlx enhancer

Depositor

Gordon Fishell

Article

Wu et al bioRxiv 2024.07.20.604399

Insert

ChR2-mCherry

Use

AAV

Expression

Mammalian

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMP71-IFN-B

Plasmid

#174597

Purpose

Expresses secreted murine interferon beta

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

secreted IFN-beta

Expression

Mammalian

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pME-Lifeact

Plasmid

#109545

Purpose

Multisite gateway vector for 5' tagging with the actin binding motif Lifeact

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actin binding motif Lifeact

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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ER2-mhYFP

Plasmid

#186528

Purpose

Localization and retention of monomeric hyperfolder YFP fluorescent protein in the ER; improved targeting efficiency

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Monomeric hyperfolder YFP

Tags

Calreticulin and KDEL

Expression

Mammalian

Mutation

hfYFP-S147P/L195M/V206K

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV10-3xFLAG-prs-XK

Plasmid

#184369

Purpose

Express human XK in Expi293 cells

Depositor

Karin Reinisch

Article

Adlakha et al J Cell Biol. 2022 Jun 6;221(5):e20220203

Insert

Human XK (XK Human)

Tags

3xFLAG-PreScission cleavage site

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB (TIREVOL)-sfGFP

Plasmid

#189675

Purpose

titratable expression of sfGFP in bacteria

Depositor

Daniel Daley

Article

Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

Insert

sfGFP

Tags

His-T7-EK

Expression

Bacterial

Promoter

pBAD

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAG328 td-iFAST

Plasmid

#130811

Purpose

Expresses td-iFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Biochemistry. 2018 Oct 2;57(39):5648-565

Insert

td-iFAST

Tags

myc-tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-ro1GFP

Plasmid

#82369

Purpose

Expression of redox sensitive GFP in mammalian cells

Depositor

S. James Remington

Article

Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

Insert

ro1GFP

Expression

Mammalian

Mutation

C48S/F64L/T65S/S147C/Q204C

Promoter

CMV

Available Since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pT/CMV-SV40-LgT

Plasmid

#20204

Depositor

John Ohlfest

Article

Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

Insert

SV40LgT

Use

Transposon

Available Since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

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pLX-AS

Plasmid

#188870

Purpose

Compact T-DNA binary vector of the pLX series suitable for assembly of plant virus infectious clones and their Agrobacterium-mediated delivery to plants

Depositor

Fabio Pasin

Article

Pasin et al STAR Protoc. 2022 Dec 16;3(4):101716. do

Insert

none

Use

Synthetic Biology; Binary t-dna vector

Expression

Plant

Promoter

cauliflower mosaic virus (CaMV) 35S promoter

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-ALFA

Plasmid

#198301

Purpose

Produces PVCs retargeted with ALFA tag

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-ALFA

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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[pSK581] pRK5 HA-SLC38A9N

Plasmid

#136141

Purpose

mammalian expression of SLC38A9 N term fragment (1-119)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

Insert

SLC38A9 N term fragment (1-119) (SLC38A9 Human)

Tags

HA

Expression

Mammalian

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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LZF40: hSyn-Cre

Plasmid

#104124

Purpose

synOptopatch construct

Depositor

Adam Cohen

Article

Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

Insert

Cre

Use

Lentiviral

Expression

Mammalian

Promoter

human synapsin I

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Casp7 C186A-Flag

Plasmid

#11816

Depositor

Guy Salvesen

Article

Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

Insert

Caspase 7 catalytic mutant (CASP7 Human)

Tags

Flag

Expression

Mammalian

Mutation

C186A

Available Since

May 12, 2006

Availability

Academic Institutions and Nonprofits only
pBAD/HisB (TIREVOL)-mNG

Plasmid

#189722

Purpose

titratable expression of mNeonGreen in bacteria

Depositor

Daniel Daley

Article

Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

Insert

mNeonGreen

Tags

His-T7-EK

Expression

Bacterial

Promoter

pBAD

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-ZapCY1

Plasmid

#36319

Depositor

Amy Palmer

Article

Qin et al Proc Natl Acad Sci U S A. 2011 May 3;108

Insert

ZapCY1 high affinity Zn(II) sensor

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2012

Availability

Academic Institutions and Nonprofits only

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pRsetB-RoTq-Off

Plasmid

#234466

Purpose

Bacterial expression vector for RoTq-Off

Depositor

Gary Yellen

Article

Rosen et al Proc Natl Acad Sci U S A. 2025 May 13;12

Insert

RoTq-Off

Tags

7xHis

Expression

Bacterial

Available Since

May 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-CaM-V5-sTb(N)

Plasmid

#219779

Purpose

sTb(N) half of CaST for HEK cell expression

Depositor

Christina Kim

Article

Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

Insert

CaM-V5-sTb(N)

Use

AAV

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCA_e-1

Plasmid

#202096

Purpose

Receiver cloning vector for even-1 level constructs. Contains lacZ for blue/white screening.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0R_lacZ

Plasmid

#202095

Purpose

Receiver cloning vector for making level 0 parts. Contains lacZ for blue/white screening.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCA_o-1

Plasmid

#202091

Purpose

Receiver cloning vector for odd-1 level constructs. Contains lacZ for blue/white screening.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCTCON2-BXG

Plasmid

#158127

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

mTagBFP

Expression

Yeast

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pAG1244 p65-mCherry-FIREtag

Plasmid

#205175

Purpose

Expresses p65-mCherry-FIREtag in mammalian cells

Depositor

Arnaud Gautier

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Insert

p65-mCherry-FIREtag

Expression

Mammalian

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pRST11B-AS3_4

Plasmid

#102789

Purpose

Expresses tryptophanyl tRNA synthetase (ScWRS) and supressor tryptophanyl tRNA

Depositor

Andrew Ellington

Article

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Inserts

tryptophanyl tRNA synthetase (WRS1 Budding Yeast)
suppressor tRNA

Expression

Bacterial

Available Since

July 3, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pLV-EF1a-LIC-V5

Plasmid

#120248

Purpose

For LIC cloning with C-terminal V5 tag

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Type

Empty backbone

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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p3E-EGFP

Plasmid

#140877

Purpose

126573: Multisite Gateway middle entry vector for fusing mScarlet to the 3' end of an ORF.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

EGFP

Use

Gateway entry clone

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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peZinCh-2

Plasmid

#70008

Purpose

expresses eZinCh-2 Zn2+ FRET sensor in the cytosol of mammalian cells

Depositor

Maarten Merkx

Article

Hessels et al ACS Chem Biol. 2015 Sep 18;10(9):2126-34

Insert

eZinCh-2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 9, 2015

Availability

Academic Institutions and Nonprofits only

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