We narrowed to 16,915 results for: EMB

1. pFUS_B9

   Plasmid

   #31026

   Depositor

   Adam Bogdanove, Daniel Voytas

   Article

   Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

   Insert

   LacZ + BsaI restriction sites
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMaCTag-18

   Plasmid

   #119997

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (without selection) with mKeima8.5

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mKeima8.5
   

   Use

   Pcr template

   Tags

   mKeima8.5

   Expression

   Mutation

   Promoter

   None

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMTB-Multibow-mG

   Plasmid

   #60987

   Purpose

   Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound EGFP

   Depositor

   Sean Megason

   Article

   Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

   Insert

   EGFP
   

   Use

   Zebrafish

   Tags

   membrane tag

   Expression

   Mutation

   Promoter

   Bactin2

   Available since

   Jan. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

4. SNAP-EF(N)-30nmSAH-GFP(1-10)

   Plasmid

   #61662

   Purpose

   30nm SAH scaffold with EF Hand and split GFP attachment sites

   Depositor

   Kristen Verhey

   Article

   Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

   Insert

   SNAP-EF(N)-30nmSAH-GFP(1-10)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSG75-β2AR-T4L-sfGFP

   Plasmid

   #102461

   Purpose

   Expresses a fusion protein of β2 adrenergic receptor/T4 lysozyme chimera and sfGFP with a C-terminal His tag. The expression is controlled by a OR2-OR1-Pr promoter.

   Depositor

   Richard Murray

   Article

   Guo et al bioRxiv 104455

   Insert

   β2AR
   

   Use

   Tags

   His tag and sfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   OR2-OR1-Pr

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cry2PHR - IBAR

   Plasmid

   #176602

   Purpose

   Cry2PHR with C-terminal IBAR domain

   Depositor

   Robert Hughes

   Article

   Wurz et al J Biol Chem. 2022 Aug 18;298(10):102388.

   Insert

   Cry2PHR-mCherry-IBAR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAG-ERas-IH

   Plasmid

   #15688

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Nature. 2003 May 29. 423(6939):541-5.

   Insert

   ERas (Eras Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pMXs-EGFP-Rheb SSVM-IP

   Plasmid

   #13832

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

   Insert

   Rheb SSVM (Rheb Mouse)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C181 changed to serine

   Promoter

   Available since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pMaCTag-01

   Plasmid

   #119980

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (without selection) with moxBFP

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   moxBFP
   

   Use

   Pcr template

   Tags

   moxBFP

   Expression

   Mutation

   Promoter

   None

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pACEBac1-NEDD1

    Plasmid

    #178073

    Purpose

    Insect cell expression of NEDD1

    Depositor

    Tarun Kapoor

    Article

    Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

    Insert

    NEDD1 (NEDD1 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFUS_B7

    Plasmid

    #31024

    Depositor

    Adam Bogdanove, Daniel Voytas

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    LacZ + BsaI restriction sites
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. C453-E23: attB4-TRE3Gp>-attB5

    Plasmid

    #162979

    Purpose

    Gateway attB4/attB5 entry clone for inducible mammalian expression using the TRE3G promoter

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    Tet-regulated TRE3G minimal promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. GGE021

    Plasmid

    #120778

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for junction of position2-3. When using the GG platform for expressing only two gene (T2-3_Lip2)

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    T2-3_Lip2
    

    Use

    Cloning/assembly plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSP64-H2B-GFP

    Plasmid

    #135605

    Purpose

    Expression of H2B-GFP mRNA for microinjection to visualize Histone 2B protein dynamics in S. purpuratus

    Depositor

    Mamiko Yajima

    Article

    Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

    Insert

    H2B-GFP
    

    Use

    In vitro transcription (mrna synthesis)

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Unknown

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRS426-GFP-Num1-PH

    Plasmid

    #71374

    Purpose

    For analysis of PH domain localization in yeast

    Depositor

    Mark Lemmon

    Article

    Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

    Insert

    Num1-PH
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Pleckstrin homology (PH) domain; aa2563-2692

    Promoter

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

16. TOPO Venus

    Plasmid

    #68493

    Purpose

    TOPO construct expressing Venus for generation of GMAP-compatible gene

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    split Venus
    

    Use

    Gmap

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDD398

    Plasmid

    #91832

    Purpose

    AID NT-tag linker donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    Auxin-inducible degron (AID)
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFastBac GST-STIL

    Plasmid

    #80265

    Purpose

    baculovirus creation plasmid for GST-STIL

    Depositor

    Andrew Holland

    Article

    Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

    Insert

    STIL (STIL Human)
    

    Use

    Tags

    GST

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOGG031

    Plasmid

    #113994

    Purpose

    Level 0 module. IPTG promoter for inducible expression

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    IPTG-inducible promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOGG016

    Plasmid

    #113990

    Purpose

    Vector construction module. Endlinker/terminator module to circularize plasmid following Part 6

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    Endlinker/terminator Part 6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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