We narrowed to 7,067 results for: ef1a;

1. pAAV-EF1a-pMagHigh-FlpC

   Plasmid

   #180586

   Purpose

   PA-Flp

   Depositor

   Won Do Heo

   Article

   Jung et al Nat Commun. 2019 Jan 18;10(1):314. doi:

   Insert

   Split FlpO
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-EF1alpha-dCas9-p300_Blast

   Plasmid

   #192653

   Purpose

   3rd generation lenti vector encoding dCas9-p300 with 2A Blast resistance marker

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   dCas9-p300
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A; H840A in Cas9

   Promoter

   EF1a

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Ef1a-fDIO-tdTomato

   Plasmid

   #128434

   Purpose

   Expresses tdTomato under control of eukaryotic Ef1a promoter after Flp-dependent recombination

   Depositor

   Patricia Jensen

   Article

   Sciolino et al Sci Adv. 2022 Aug 19;8(33):eabn9134. doi

   Has Service

   AAV1

   Insert

   tdTomato
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-EF1A-LivePAR-Hygro

   Plasmid

   #176063

   Purpose

   EGFP fused to the C-terminus of a WWE domain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   LivePAR
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-pEF1a-EBFP-tTAn

   Plasmid

   #172122

   Purpose

   Constitutive expression of EBFP and rtTA-InteinN

   Depositor

   Chun Xu

   Article

   Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

   Insert

   EBFP, tTAn-InteinN
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEF1a-PEmax-P2A-GFP

   Plasmid

   #202000

   Purpose

   Based on the pCMV-PEmax-P2A-GFP, we replaced the CMP promoter with the EF1a promoter for expression in iPSC.

   Depositor

   Daniel Geschwind

   Insert

   PEmax-P2A-GFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHLS-EF1a-FRB-SpCas9-A

   Plasmid

   #138477

   Purpose

   Expresses FRB fused SpCas9 for NanoMEDIC packaging.

   Depositor

   Akitsu Hotta

   Article

   Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

   Insert

   SpCas9, human codon optimized
   

   Use

   CRISPR

   Tags

   3x HA tag and FRB

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. EF1a-EGFP (no signal control)

   Plasmid

   #170167

   Purpose

   Mammalian Expression of no signal control

   Depositor

   Christopher Moxham

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   Available since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOTTC1328 - pAAV EF1a HA-hM3D(Gq)

   Plasmid

   #89149

   Purpose

   An AAV packaging plasmid that expresses HA-tagged hM3D(Gq) excitatory DREADD from the EF1a promoter.

   Depositor

   Brandon Harvey

   Article

   Gomez et al Science. 2017 Aug 4;357(6350):503-507. d

   Insert

   hM3D(Gq)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSicoR-Ef1a-mCh-Puro

    Plasmid

    #31845

    Purpose

    Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression. Includes puromycin selection.

    Depositor

    Bruce Conklin

    Article

    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA; Ef1alpha for mCherry-puromycin

    Available since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAF0084 Ef1a-Cp36-2a-EGFP

    Plasmid

    #193462

    Purpose

    Expression of Cp36 recombinase from Ef1a promoter

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Cp36
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSmart-EF1a-Nla-cas3

    Plasmid

    #178881

    Purpose

    Expression of human codon-optimized NlaCas3 with C terminal NLS and HA tag

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Insert

    EF1a-NlaCas3c-bGH polyA
    

    Use

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPV-EF1a-mRxxFP1(AfeI)-iP-A

    Plasmid

    #204625

    Purpose

    Cloning vector for SSA vector (mRxxFP), the target sequence can be inserted by In-Fusion reaction after AfeI digestion

    Depositor

    Akitsu Hotta

    Article

    Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Ef1a-fDIO-GCaMP6s

    Plasmid

    #105714

    Purpose

    Can be used to express GCaMP6s in the presence of Flp recombinase. Can be used to create adeno-associated virus to express Flp-dependent GCaMP6s.

    Depositor

    Rylan Larsen

    Has Service

    AAV8

    Insert

    GCaMP6s
    

    Use

    AAV; Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a, Human elongation factor-1 alpha

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EF1a-DIO-mGreenLantern

    Plasmid

    #164468

    Purpose

    Cre-dependent EF1a-driven expression of mGreenLantern in animals using AAV

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

    Promoter

    EF1a

    Available since

    Jan. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-Ef1a-InteinC-CreC

    Plasmid

    #187615

    Purpose

    AAV vector encoding C-terminal portion of split-Intein-mediated split-Cre

    Depositor

    Il Hwan Kim

    Article

    Kim et al Cell Rep. 2022 Jun 7;39(10):110906. doi:

    Insert

    InteinC-CreC
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-Ef1a-DIO mScarlet

    Plasmid

    #131002

    Purpose

    Double floxed mScarlet under the control of Ef1a promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Has Service

    AAV1 and AAV5

    Insert

    mScarlet
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPV-EF1a-EGxxFP(DMDex45_10)-iP-A

    Plasmid

    #204621

    Purpose

    Single-strand annealing (SSA) vector for monitoring crRNA(DMDex45)'s activity. DMD intron 44 region (upstream of exon 45)

    Depositor

    Akitsu Hotta

    Article

    Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-Ef1a-MCP-huADARE488QT490A

    Plasmid

    #159922

    Purpose

    Fusion of MS2 coat protein to Human ADAR2 E488QT490A, under Ef1a promoter, with WPRE. AKA 116v5

    Depositor

    Fei Chen

    Article

    Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

    Insert

    Human ADAR2 E488QT490A (ADARB1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV-Ef1a-CreN-InteinN

    Plasmid

    #187614

    Purpose

    AAV vector that encodes N-terminal portion of split-Intein-mediated split-Cre

    Depositor

    Il Hwan Kim

    Article

    Kim et al Cell Rep. 2022 Jun 7;39(10):110906. doi:

    Insert

    CreN-InteinN
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge