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Plasmid#226841PurposeExpresses NXPE1 protein residues 60-547 in mammalian cells (HEK293T or expi293). x6 His tag and IL2 secretion signal included N terminal with GGGG linker to protein sequence.DepositorInsertNXPE1 (NXPE1 Human)
UseTagsx6 HIS and an IL2 secretion signal both N termina…ExpressionMammalianMutationCodon optimized for expression in human cellsPromoterAvailable sinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PRSX8ChR2 (H134R)eYFP
Plasmid#89539PurposeExpresses ChR2 (H134R) -eYFP in Phox2 (including catecholamine) neuronsDepositorInsertChR2 (H134R)
UseLentiviralTagsEYFPExpressionMammalianMutationPromoterAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSM08
Plasmid#139042PurposeMosSCI SapTrap assembly: Cassette 3 for 4-cassette systemDepositorInsertmscarlet (includes STOP codon)
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAC-Kan-alphaGal4
Plasmid#13421DepositorInsertRNAP alpha-Gal4
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pXF88
Plasmid#139039PurposeMosSCI SapTrap assembly: Cassette 3 for 4-cassette systemDepositorInserthalotag (includes STOP codon, PATC introns)
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET24c(+)-mtEFTu
Plasmid#30535DepositorInsertMature bovine mitochondrial elongation factor Tu
UseTagsHisExpressionBacterialMutationMature form amino acids 44-452. Mito import seque…PromoterAvailable sinceOct. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJF6
Plasmid#139040PurposeMosSCI SapTrap assembly: Cassette 3 for 4-cassette systemDepositorInsertgfp (includes STOP codon, PATC introns)
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL4_NFAT-RE-CMV-BC0527-luc2
Plasmid#214657Purposebarcoded assay, Ca2+ sensorDepositorInsert6x clustered NFAT element with barcode 0527
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSM03
Plasmid#139043PurposeMosSCI SapTrap assembly: Cassette 3 for 4-cassette systemDepositorInsertdendra2 (includes STOP codon)
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T_PDZ2
Plasmid#59735Purposeexpresses PDZ2 domain of human ZO-1 in bacteriaDepositorInsertzonula occludens-1 (TJP1 Human)
UseTagsGST and thrombin clvgExpressionBacterialMutationPromoterAvailable sinceSept. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTol2-elavl3_NES-rsCaMPARI-mRuby3
Plasmid#122129PurposePan-neuronal expression of rsCaMPARI in zebrafish, nucleus excludedDepositorInsertrsCaMPARI
UseZebrafish expressionTagsNES-His and mRuby3ExpressionMutationPromoterelavl3Available sinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL4_CRE-CMV-BC0482-luc2
Plasmid#214635Purposebarcoded assay, cAMP/Ca2+ sensorDepositorInsert6x clustered CRE element with barcode 0482
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNCS-Flag
Plasmid#17364DepositorInsertMMLV proviral DNA
UseTagsFlagExpressionMutationPromoterAvailable sinceMarch 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ClfB_N2N3-HIS-ybbr
Plasmid#101717PurposeClfB N2 and N3 domains with C-terminal ybbr tag for covalent immobilizationDepositorInsertClfB_N2N3
UseTagsH3V 3C, HIS, and ybbrExpressionBacterialMutationPromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
HOXA1 (murine) HIS-tag pET
Plasmid#8556DepositorInsertHOXA1 (Hoxa1 Mouse)
UseTagsHis and T7ExpressionBacterialMutationclone contains 130 AA including the homeodomain, …PromoterAvailable sinceJuly 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pXF130
Plasmid#139041PurposeMosSCI SapTrap assembly: Cassette 3 for 4-cassette systemDepositorInsertmkate2 (includes STOP codon)
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXf20pemIK
Plasmid#126577PurposeVector for gene expression in Xylella fastidiosa, includes pemI/pemK toxin-antitoxin system for plasmid stabilityDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLSLGUS
Plasmid#51517PurposepLSL with GUS coding sequence followed by 3 kb of filler sequence Rep is not included on this plasmidDepositorInsertsGUS
3kb filler sequence
UsePlant t-dna plasmidTagsNLS and flagExpressionMutationPromotervirion-sense LIR and 2x35SAvailable sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL3-basic-hFX-promotor(2kb, distal)
Plasmid#14981DepositorInserthuman frataxin promoter fragment (FXN Human)
UseLuciferaseTagsExpressionMutationincludes distal fragment of human frataxin promot…PromoterAvailable sinceAug. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJLI-sup35
Plasmid#1210DepositorInsertsup35 5' and 3' flanking regions (SUP35 Budding Yeast)
UseTagsExpressionYeastMutationsup35 gene is not included in this plasmid. Only…PromoterAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pSM05
Plasmid#139050PurposeMosSCI SapTrap assembly: Cassette 3B for 5-cassette systemDepositorInsertmkate2 (includes STOP codon)
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLSLT
Plasmid#51498PurposepLSL with T30 TALEN followed by us:NPTII repair template, Rep is not included on this plasmidDepositorInsertsT30 TALEN pair
us:NPTII repair template
UsePlant t-dna plasmidTagsExpressionMutationPromotervirion-sense LIR and 2x35SAvailable sinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
miniUAA1
Plasmid#78480Purpose488nt RNA is encoded by chimaeric gene cloned in a pSP65A vector that included about 65 dT residues for transcription of a poly(A) tailDepositorInsertCAN1
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
UAA
Plasmid#78486Purposestop codon-containing chimaeric gene cloned in a pSP65A vector that included about 65 dT residues for transcription of a poly(A) tailDepositorInsertCAN1-LUC
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
EphA1-SAM-Cry2olig
Plasmid#176600PurposeEphrinA1 RTK fusion to Cry2olig - includes SAM domainDepositorInsertEphrinA1-Cry2olig-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pm-EphA1-SAM-Cry2olig
Plasmid#176684Purposemembrane localized EphrinA1 RTK fusion to Cry2olig - includes SAM domainDepositorInsertPM-EphA1-Cry2olig-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-1~41-mEGFP(A206K)-TwStrep
Plasmid#208628Purposefor E. coli expression of truncated human RPL3 (ENST00000216146), aa1~41, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 1~41 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 1~41, other residues not includedPromoterT7Available sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_CRE-CMV-BC0484-luc2
Plasmid#214637Purposebarcoded assay, cAMP/Ca2+ sensorDepositorInsert6x clustered CRE element with barcode 0484
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_NFAT-RE-CMV-BC0529-luc2
Plasmid#214659Purposebarcoded assay, Ca2+ sensorDepositorInsert6x clustered NFAT element with barcode 0529
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_NFAT-RE-CMV-BC0537-luc2
Plasmid#214662Purposebarcoded assay, Ca2+ sensorDepositorInsert6x clustered NFAT element with barcode 0537
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_NFAT-RE-CMV-BC0533-luc2
Plasmid#214661Purposebarcoded assay, Ca2+ sensorDepositorInsert6x clustered NFAT element with barcode 0533
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_CRE-CMV-BC0502-luc2
Plasmid#214644Purposebarcoded assay, cAMP/Ca2+ sensorDepositorInsert6x clustered CRE element with barcode 0502
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_CRE-CMV-BC0483-luc2
Plasmid#214636Purposebarcoded assay, cAMP/Ca2+ sensorDepositorInsert6x clustered CRE element with barcode 0483
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_CRE-CMV-BC0495-luc2
Plasmid#214642Purposebarcoded assay, cAMP/Ca2+ sensorDepositorInsert6x clustered CRE element with barcode 0495
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_CRE-CMV-BC0497-luc2
Plasmid#214643Purposebarcoded assay, cAMP/Ca2+ sensorDepositorInsert6x clustered CRE element with barcode 0497
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_CRE-CMV-BC0487-luc2
Plasmid#214638Purposebarcoded assay, cAMP/Ca2+ sensorDepositorInsert6x clustered CRE element with barcode 0487
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_CRE-CMV-BC0490-luc2
Plasmid#214641Purposebarcoded assay, cAMP/Ca2+ sensorDepositorInsert6x clustered CRE element with barcode 0490
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_CRE-CMV-BC0488-luc2
Plasmid#214639Purposebarcoded assay, cAMP/Ca2+ sensorDepositorInsert6x clustered CRE element with barcode 0488
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_CRE-CMV-BC0489-luc2
Plasmid#214640Purposebarcoded assay, cAMP/Ca2+ sensorDepositorInsert6x clustered CRE element with barcode 0489
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_NFAT-RE-CMV-BC0528-luc2
Plasmid#214658Purposebarcoded assay, Ca2+ sensorDepositorInsert6x clustered NFAT element with barcode 0528
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_NFAT-RE-CMV-BC0530-luc2
Plasmid#214660Purposebarcoded assay, Ca2+ sensorDepositorInsert6x clustered NFAT element with barcode 0530
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_NFAT-RE-CMV-BC0546-luc2
Plasmid#214664Purposebarcoded assay, Ca2+ sensorDepositorInsert6x clustered NFAT element with barcode 0546
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_NFAT-RE-CMV-BC0538-luc2
Plasmid#214663Purposebarcoded assay, Ca2+ sensorDepositorInsert6x clustered NFAT element with barcode 0538
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL4_NFAT-RE-CMV-BC0526-luc2
Plasmid#214656Purposebarcoded assay, Ca2+ sensorDepositorInsert6x clustered NFAT element with barcode 0526
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-53~77-mEGFP(A206K)-TwStrep
Plasmid#208632Purposefor E. coli expression truncated human RPL3 (ENST00000216146), aa53~77, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 53~77 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 53~77, other residues not includedPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-RPL3-1~77-mEGFP(A206K)-TwStrep
Plasmid#208624Purposefor E. coli expression truncated human RPL3 (ENST00000216146), aa1~77, fused with mEGFP(A206K) and Twin Strep tagDepositorInserthuman RPL3 (ENST00000216146) aa 1~77 (RPL3 Human)
UseTags6xHis, Twin Strep, and mEGFP(A206K)ExpressionBacterialMutationonly aa 1~77, other residues not includedPromoterT7Available sinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
F5SJF3
Plasmid#163233PurposeInducible expression of UniProt identifier F5SJF3DepositorInsertF5SJF3
UseTags10xHis and T7ExpressionBacterialMutationPromoterT7Available sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH123
Plasmid#172146PurposeMicroscopy inclusion body assayDepositorInsertpCDF.B.(SD4)CD
UseTagsExpressionBacterialMutationPylBCD operon (RBS inserted for PylC)PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSM-dCas9::Ap
Plasmid#154307PurposeShuttle vector for expression dCas9, mobilization cluster, expresses the tracrRNA and a CRISPR array designed for the easy cloning of new spacers.DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXf20pemIK-GW
Plasmid#126576PurposeVector for gene expression in Xylella fastidiosa, includes pemI/pemK toxin-antitoxin system for plasmid stability, Gateway cloning compatibleDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only