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Showing: 1501 - 1550 of 1934 results
  1. pCEP-Puro-TetOn-L1RP-ORF1_R261A_Halo-GFPai

    Plasmid
    #202569
    Purpose
    Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the R261A mutation and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR
    Depositor
    Insert
    LINE-1
    Use
    Tags
    GFP-AI retrotransposition reporter and HaloTag7 o…
    Expression
    Mammalian
    Mutation
    Changed ORF1p Arginine 261 to Alanine
    Promoter
    Available since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. pAC-NEUR

    Plasmid
    #53271
    Purpose
    Contains the Erwinia herbicola (Pantoea agglomerans) Eho10 genes crtE and crtB, and the Rhodobacter capsulatus crtI gene and thereby produces neurosporene in Escherichia coli
    Insert
    crtI
    Use
    Low copy number bacterial cloning vector
    Tags
    Expression
    Mutation
    Promoter
    endogenous promoters
    Available since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
  3. p123shy1-mCherry-HA

    Plasmid
    #67875
    Purpose
    encodes shy1 with a C-terminal mCherry-HA tag under control of its endogenous promoter.It's suitable for localization studies in Ustilago maydis.
    Depositor
    Insert
    shy1
    Use
    Expression in ustilago maydis
    Tags
    HA and mCherry
    Expression
    Mutation
    Promoter
    shy1
    Available since
    Aug. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
  4. pKDR-TetOn.1

    Plasmid
    #183578
    Purpose
    One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated Tet-On promoter, Fig 3)
    Depositor
    Insert
    Attenuator Sequence 1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tet-On
    Available since
    June 30, 2022
    Availability
    Academic Institutions and Nonprofits only
  5. pKDR-TetOn.4

    Plasmid
    #183579
    Purpose
    One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated Tet-On promoter, Fig 3)
    Depositor
    Insert
    Attenuator Sequence 4
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tet-On
    Available since
    June 27, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. pKDR-EF1a.4

    Plasmid
    #183571
    Purpose
    One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)
    Depositor
    Insert
    Attenuator Sequence 4
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pKDR-EF1a.2

    Plasmid
    #183569
    Purpose
    One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (attenuated EF1a promoter, Fig 3)
    Depositor
    Insert
    Attenuator Sequence 2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 24, 2022
    Availability
    Academic Institutions and Nonprofits only
  8. GTT-g

    Plasmid
    #81109
    Purpose
    Gene Tagging vector TRP/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)
    Depositor
    Insert
    sfGFP
    Use
    Cre/Lox
    Tags
    sfGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
  9. GTH-r

    Plasmid
    #81106
    Purpose
    Gene Tagging vector HIS/RFP - Plasmid for monomeric Cherry (mCherry) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)
    Depositor
    Insert
    mCherry
    Use
    Cre/Lox
    Tags
    mCherry
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
  10. GTH-g

    Plasmid
    #81104
    Purpose
    Gene Tagging vector HIS/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)
    Depositor
    Insert
    sfGFP
    Use
    Cre/Lox
    Tags
    sfGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
  11. GTL-g

    Plasmid
    #81099
    Purpose
    Gene Tagging vector LEU/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)
    Depositor
    Insert
    sfGFP
    Use
    Cre/Lox
    Tags
    sfGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
  12. GTT-r

    Plasmid
    #81111
    Purpose
    Gene Tagging vector TRP/RFP - Plasmid for monomeric Cherry (mCherry) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)
    Depositor
    Insert
    mCherry
    Use
    Cre/Lox
    Tags
    mCherry
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
  13. GTU-r

    Plasmid
    #81096
    Purpose
    Gene Tagging vector URA/RFP - Plasmid for monomeric Cherry (mCherry) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)
    Depositor
    Insert
    mCherry
    Use
    Cre/Lox
    Tags
    mCherry
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
  14. GTL-r

    Plasmid
    #81195
    Purpose
    Gene Tagging vector LEU/RFP - Plasmid for monomeric Cherry (mCherry) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)
    Depositor
    Insert
    mCherry
    Use
    Cre/Lox
    Tags
    mCherry
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
  15. miR-144-451 Trap

    Plasmid
    #61488
    Purpose
    to knockdown endogenous miR144 and miR451 levels
    Depositor
    Insert
    miR-144-451 Trap
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    bidirectional phosphoglycerate kinase promoter (b…
    Available since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
  16. XW83

    Plasmid
    #65841
    Purpose
    MosSCI-Pmig-13::QF::SL2::mCherry
    Depositor
    Insert
    OR74A quinic acid utilization activator (qa-1F)
    Use
    Tags
    mCherry
    Expression
    Worm
    Mutation
    The endogenous SphI, KpnI and SacI sites in QF ar…
    Promoter
    Available since
    Oct. 19, 2015
    Availability
    Academic Institutions and Nonprofits only
  17. NBS1-Halo HRD

    Plasmid
    #207084
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous NBS1 locus.
    Depositor
    Insert
    HaloTag followed by a LoxP-PuroR cassette flanked by human NBS1 locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    None
    Available since
    May 15, 2025
    Availability
    Academic Institutions and Nonprofits only
  18. Rev7-Halo HRD

    Plasmid
    #207079
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous REV7 locus.
    Depositor
    Insert
    HaloTag followed by a PolyA signal and PuroR cassette flanked by human REV7 locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    None
    Available since
    May 1, 2025
    Availability
    Academic Institutions and Nonprofits only
  19. Halo-ATM HRD

    Plasmid
    #207085
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous ATM locus.
    Depositor
    Insert
    HaloTag with internal PuroR cassette flanked by human ATM locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    None
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
  20. ATM-Halo HRD

    Plasmid
    #207082
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous ATM locus.
    Depositor
    Insert
    HaloTag followed by a PolyA signal and PuroR cassette flanked by human ATM locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    None
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
  21. Rif1-Halo HRD

    Plasmid
    #207081
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RIF1 locus.
    Depositor
    Insert
    HaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    None
    Available since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 1501 - 1550 of 1934 results