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We narrowed to 4,233 results for: Gca

Showing: 1501 - 1550 of 4233 results

  1. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR GLIS3 TSS-guide2

    Plasmid
    #118174
    Purpose
    CRISPR-mediated repression of GLIS3. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK and U6
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118174-plasmid-map-sequence-id-240378

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  2. pET21b(+)_SARS-CoV-2_3CLpro_Q306A-HIS

    Plasmid
    #224697
    Purpose
    Bacterial Expression and Purification of SARS-CoV-2 3CLpro active protease with His-tag
    Depositor
    Christopher Overall
    Article
    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    Use
    Tags
    Gly-Gly-6xHis
    Expression
    Bacterial
    Mutation
    silent mutation C to T at position 10546 (elimina…
    Promoter
    T7
    Available since
    July 2, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    224697-plasmid-map-sequence-id-444276

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  3. pET21b(+)_SARS-CoV-2_3CLpro-Q306A

    Plasmid
    #177334
    Purpose
    bacterial expression of active SARS-CoV-2 3C-like proteinase
    Depositor
    Christopher Overall
    Article
    Pablos et al Cell Rep. 2021 Oct 26;37(4):109892. doi:
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    Use
    Tags
    Factor Xa site-3xFlag-Myc-6xHis
    Expression
    Bacterial
    Mutation
    silent mutation C to T at position 10546 (elimina…
    Promoter
    T7
    Available since
    Nov. 23, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    177334-plasmid-map-sequence-id-348602

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  4. pET21b(+)_SARS-CoV-2_3CLpro_C145A-HIS

    Plasmid
    #224698
    Purpose
    Bacterial Expression and Purification of SARS-CoV-2 3CLpro inactive protease with His-tag
    Depositor
    Christopher Overall
    Article
    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi
    Insert
    SARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
    Use
    Tags
    Gly-Gly-6xHis
    Expression
    Bacterial
    Mutation
    silent mutation C to T at position 10546 (elimina…
    Promoter
    T7
    Available since
    July 2, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    224698-plasmid-map-sequence-id-444264

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  5. pJMK019

    Plasmid
    #42168
    Depositor
    Adam Cohen
    Insert
    CaViar
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    42168-plasmid-map-sequence-id-56914

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  6. pcDNA3 metaxin 1

    Plasmid
    #12418
    Depositor
    Paul Bornstein
    Insert
    metaxin (Mtx1 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 23, 2006
    Availability
    Academic Institutions and Nonprofits only

  7. LHZ1493

    Plasmid
    #222102
    Purpose
    Cloning backbone for sgRNA in Kluyveromyces marxianus. Expresses highly specific SlugCas9.
    Depositor
    Yongming Wang
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    222102-plasmid-map-sequence-id-441240

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  8. pTJK459

    Plasmid
    #138008
    Purpose
    Empty sgRNA expression vector for expression of sgRNA for Sp Cas9
    Depositor
    Tami Kingsbury
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    U-A flip and hairpin extension: cgtttAagagctaTGCT…
    Promoter
    Available since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    138008-plasmid-map-sequence-id-275002

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  9. pAAV Syn Barcode16

    Plasmid
    #226195
    Purpose
    Expression mapping
    Depositor
    Scott Sternson
    Insert
    Syn Barcode16
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226195-plasmid-map-sequence-id-447891

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  10. pAAV Syn Barcode15

    Plasmid
    #226189
    Purpose
    Expression mapping
    Depositor
    Scott Sternson
    Insert
    Syn Barcode15
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    226189-plasmid-map-sequence-id-447880

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  11. pAAV Syn Barcode13

    Plasmid
    #226187
    Purpose
    Expression mapping
    Depositor
    Scott Sternson
    Insert
    Syn Barcode13
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    226187-plasmid-map-sequence-id-447869

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  12. pOpen-PstIR

    Plasmid
    #165526
    Purpose
    Restriction enzyme that recognizes the double-stranded sequence CTGCAG and cleaves after A-5.
    Depositor
    Jennifer Molloy, Drew Endy, FreeGenes Project
    Insert
    PstI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165526-plasmid-map-sequence-id-339060

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  13. LHZ1494

    Plasmid
    #222104
    Purpose
    Triple sgRNAs expression plasmid in Kluyveromyces marxianus.
    Depositor
    Yongming Wang
    Insert
    sg1RNA, sg4RNA, sg7RNA
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Nov. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222104-plasmid-map-sequence-id-454353

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  14. pAAV Syn Barcode17

    Plasmid
    #226190
    Purpose
    Expression mapping
    Depositor
    Scott Sternson
    Insert
    Syn Barcode17
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226190-plasmid-map-sequence-id-447877

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  15. pAAV Syn Barcode11

    Plasmid
    #226185
    Purpose
    Expression mapping
    Depositor
    Scott Sternson
    Insert
    Syn Barcode11
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    226185-plasmid-map-sequence-id-447874

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  16. pTJK644

    Plasmid
    #138012
    Purpose
    SIX1 knockout
    Depositor
    Tami Kingsbury
    Insert
    SIX1 sgRNA2 (SIX1 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    None
    Promoter
    U6
    Available since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    138012-plasmid-map-sequence-id-275045

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  17. pTJK635

    Plasmid
    #138009
    Purpose
    SIX1 knockout
    Depositor
    Tami Kingsbury
    Insert
    SIX1 sgRNA2 (SIX1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    None
    Promoter
    U6
    Available since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    138009-plasmid-map-sequence-id-275004

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  18. pOpen-PstIM

    Plasmid
    #165536
    Purpose
    Methylase that recognizes the dsDNA sequence CTGCAG, methylates on A-5 on both strands, and protects DNA from cleavage by PstI.
    Depositor
    Jennifer Molloy, Drew Endy, FreeGenes Project
    Insert
    M.PstI
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165536-plasmid-map-sequence-id-339126

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  20. pDECKO_GFP_AAVS1_186

    Plasmid
    #159091
    Purpose
    targeting, non-essential locus control
    Depositor
    Rory Johnson
    Insert
    gRNA
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    159091-plasmid-map-sequence-id-308784

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  21. pDECKO_mCherry_AAVS1

    Plasmid
    #141212
    Purpose
    pDECKO_mCherry_AAVS1_186. Targeting, non-essential locus control
    Depositor
    Rory Johnson
    Insert
    gRNA
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    141212-plasmid-map-sequence-id-299605

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  22. FgH1tUTG_hup53_Ex4

    Plasmid
    #85538
    Purpose
    Inducible expression of guide RNA (hup53_Ex4) with fluorescent GFP reporter
    Depositor
    Marco Herold
    Insert
    hup53 Ex4
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    H1t
    Available since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    85538-plasmid-map-sequence-id-169844

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  24. pZNF627.1.0-gDNA

    Plasmid
    #176383
    Purpose
    Expresses spCas9 and gRNA sequence targeting ZNF627
    Depositor
    Kevin White, Barbara Stranger
    Insert
    ZNF627 gRNA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    176383-plasmid-map-sequence-id-347361

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  25. pZNF608.2.0-gDNA

    Plasmid
    #176380
    Purpose
    Expresses spCas9 and gRNA sequence targeting ZNF608
    Depositor
    Kevin White, Barbara Stranger
    Insert
    ZNF608 gRNA
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    176380-plasmid-map-sequence-id-347379

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  26. pFG018

    Plasmid
    #62817
    Purpose
    Bacterial inducible gRNA expression plasmid
    Depositor
    Sébastien Rodrigue
    Insert
    gRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    Nov. 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    62817-plasmid-map-sequence-id-114422

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  27. PX458_TGIF2_2

    Plasmid
    #72375
    Purpose
    Encodes gRNA for 3' target of human TGIF2 along with Cas9 with 2A GFP
    Depositor
    Richard M. Myers, Eric Mendenhall
    Insert
    TGIF2 (TGIF2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    72375-plasmid-map-sequence-id-132125

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  28. GE-0010-gRNA1-MpCKA

    Plasmid
    #238526
    Purpose
    Plant expression of Cas9 and gRNA against M. polymorpha CK2 alpha
    Depositor
    Michael Hothorn
    Insert
    MpCKA
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    238526-plasmid-map-sequence-id-477280

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  29. LT3GEPIR-NEK5shRNA2

    Plasmid
    #220547
    Purpose
    To inducibly knockdown NEK5 expression
    Depositor
    Sean Lee
    Insert
    NEK5 shRNA2 (NEK5 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    TRE3G (TetOP) promoter
    Available since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    220547-plasmid-map-sequence-id-437976

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  30. ZMAT4.1.1-gDNA

    Plasmid
    #175871
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White, Barbara Stranger
    Insert
    ZMAT4-Nickase1
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    175871-plasmid-map-sequence-id-346234

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  31. HEY2.1.1-gDNA

    Plasmid
    #175873
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White, Barbara Stranger
    Insert
    HEY2-Nickase1
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    175873-plasmid-map-sequence-id-346224

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  32. OVOL2.2.2-gDNA

    Plasmid
    #175877
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White, Barbara Stranger
    Insert
    OVOL2-Nicakse2
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    175877-plasmid-map-sequence-id-346194

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  33. pTHAP7.1.0-gDNA

    Plasmid
    #132439
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    THAP7 (THAP7 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    132439-plasmid-map-sequence-id-266230

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  34. mU6-sgLkb1_1st-hU6-sgNT

    Plasmid
    #177220
    Purpose
    Expresses a Lkb1-targeting and a non-targeting gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgLkb1_1st/sgNT1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    mU6/hU6
    Available since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    177220-plasmid-map-sequence-id-350037

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  35. ipUSEPR-sg-Hs-PHGDH-1

    Plasmid
    #188675
    Purpose
    sgRNA
    Depositor
    Lewis Cantley
    Insert
    sgPHGDH (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    188675-plasmid-map-sequence-id-375079

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  36. PX458_ZNF146_1

    Plasmid
    #72360
    Purpose
    Encodes gRNA for 3' target of human ZNF146 along with Cas9 with 2A GFP
    Depositor
    Richard M. Myers, Eric Mendenhall
    Insert
    ZNF146 (ZNF146 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    72360-plasmid-map-sequence-id-132110

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  37. pAAV 3xgRNA KO;Rbfox3

    Plasmid
    #240311
    Purpose
    KO:NeuN
    Depositor
    Yuki Ogawa
    Insert
    KO gRNAs for NeuN
    Use
    AAV
    Tags
    Expression
    Mutation
    NA
    Promoter
    Available since
    July 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240311-plasmid-map-sequence-id-478214

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  38. LT3GEPIR-NEK3shRNA1

    Plasmid
    #220542
    Purpose
    To inducibly knockdown NEK3 expression
    Depositor
    Sean Lee
    Insert
    NEK3 shRNA1 (NEK3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    TRE3G (TetOP) promoter
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    220542-plasmid-map-sequence-id-438390

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  39. LT3GEPIR-NEK3shRNA2

    Plasmid
    #220543
    Purpose
    To inducibly knockdown NEK3 expression
    Depositor
    Sean Lee
    Insert
    NEK3 shRNA2 (NEK3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    TRE3G (TetOP) promoter
    Available since
    July 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    220543-plasmid-map-sequence-id-438028

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  40. LT3GEPIR-NEK10shRNA2

    Plasmid
    #220557
    Purpose
    To inducibly knockdown NEK10 expression
    Depositor
    Sean Lee
    Insert
    NEK10 shRNA2 (NEK10 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    TRE3G (TetOP) promoter
    Available since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    220557-plasmid-map-sequence-id-437949

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  41. mU6-sgNT-hU6-sgLkb1_1st

    Plasmid
    #177221
    Purpose
    Expresses a Lkb1-targeting and a non-targeting gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgNT1/sgLkb1_1st
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    mU6/hU6
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    177221-plasmid-map-sequence-id-363973

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  42. mU6-sgNT-hU6-sgLkb1_2nd

    Plasmid
    #177223
    Purpose
    Expresses a Lkb1-targeting and a non-targeting gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgNT1/sgLkb1_2nd
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    mU6/hU6
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    177223-plasmid-map-sequence-id-351959

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  43. mU6-sgLkb1_2nd-hU6-sgNT

    Plasmid
    #177222
    Purpose
    Expresses a Lkb1-targeting and a non-targeting gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgLkb1_2nd/sgNT1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    mU6/hU6
    Available since
    Dec. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    177222-plasmid-map-sequence-id-350113

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  44. mU6-sgSik1_2nd-hU6-sgSik3_1st

    Plasmid
    #177218
    Purpose
    Expresses a Sik3-targeting and a non-targeting gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgSik1_2nd/sgSik3_1st
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    mU6/hU6
    Available since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    177218-plasmid-map-sequence-id-350031

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  45. mU6-sgSik1_1st-hU6-sgSik3_1st

    Plasmid
    #177216
    Purpose
    Expresses a Sik3-targeting and a non-targeting gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgSik1_1st/sgSik3_1st
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    mU6/hU6
    Available since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    177216-plasmid-map-sequence-id-350028

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  46. mU6-sgSik3_1st-hU6-sgNT

    Plasmid
    #177214
    Purpose
    Expresses a Sik3-targeting and a non-targeting gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgSik3_1st/sgNT1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    mU6/hU6
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    177214-plasmid-map-sequence-id-349897

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  47. HCP9

    Plasmid
    #166111
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets the C-terminus of Hta2
    Depositor
    Matthias Heinemann
    Insert
    Hta2-sg18 (HTA2 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166111-plasmid-map-sequence-id-330321

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  48. pZNF551.1.0-gDNA

    Plasmid
    #132441
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    ZNF551 (ZNF551 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    132441-plasmid-map-sequence-id-266318

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  49. pZNF319.1.0-gDNA

    Plasmid
    #132438
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    ZNF319 (ZNF319 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    132438-plasmid-map-sequence-id-266220

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  50. SGEP-sh-Hs-PHGDH-1956

    Plasmid
    #188670
    Purpose
    shRNA
    Depositor
    Lewis Cantley
    Insert
    shPHGDH (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    188670-plasmid-map-sequence-id-375063

    Enlarge

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