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API5-CRISPR

Plasmid

#157664

Purpose

Expresses Cas9 and sgRNA targeting API5

Depositor

Byung Il Lee

Publication

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Apoptosis inhibitor 5 (API5 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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CRY2(L348F)-CreN

Plasmid

#75368

Purpose

Expresses mCherry tag, then IRES, then Arabidopsis CRY2 (with L348F mutation) fused to N-terminal fragment of Cre DNA recombinase

Depositor

Chandra Tucker

Publication

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CRY2 (CRY2 Mustard Weed)

Tags

Cre recombinase, residues 19-104

Expression

Mammalian

Mutation

L348F

Promoter

CMV

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Echovirus 11 (E11 3C) protease A isolate E11-PLA-NECK-23-E0-62-P1-FRA23

Plasmid

#228645

Purpose

Bacterial expression of the E11 3C protease

Depositor

ASAP Discovery, Frank von Delft

Insert

Echo virus 11 3C protease

Tags

His-SUMO

Expression

Bacterial

Available since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pMV-hAT-11_AMi

Plasmid

#217261

Purpose

Donor plasmid of hAT-11_AMi Transposon

Depositor

Yong E Zhang

Publication

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

TIRs of hAT-11_AMi transposon

Expression

Mammalian

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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UKNP1.11.7

Plasmid

#97383

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Publication

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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UKNP1.11.5

Plasmid

#97381

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Publication

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available since

Aug. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pAS-9 (bulge 9-11)

Plasmid

#40844

Purpose

Target RNAs used to determine complementarity requirements for in vitro tailing and degradation

Depositor

Phillip Zamore

Publication

Ameres et al Science. 2010 Jun 18;328(5985):1534-9.

Insert

let-7–bulge 9-11

Use

Mirna reporter

Tags

EGFP

Expression

Insect

Promoter

Actin5C

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pQFlag-USP7 CS puroR

Plasmid

#46752

Purpose

Retroviral vector that expresses catalytically inactive form of Flag-tagged human USP7

Depositor

Goedele Maertens, Gordon Peters

Publication

Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

Insert

USP7 (USP7 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

C223S--catalytically inactive

Promoter

CMV

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pHH0103_TJP2-1/1

Plasmid

#91493

Purpose

Protein expression and purification of human SH3 domain construct TJP2-1/1

Depositor

Sachdev Sidhu

Publication

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

TJP2-1/1 (TJP2 Human)

Expression

Bacterial

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pDF0428 huDisCas7-11-R1045-GGGS-R1122-U6-pro-Gluc

Plasmid

#186985

Purpose

AAV transgene plasmid for Cas7-11-R1045-GGGS-R1122, with U6 promoter-driven expression of Gluc crRNA guide

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Publication

Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

Insert

cas7-11-r1045-gggs-r1122, Gluc crRNA guide

Use

AAV

Mutation

r1045-gggs-r1122 deletion

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pLH-sgRNA-Sirius-8XMS2

Plasmid

#121939

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Publication

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

sgRNA-Sirius-8XMS2

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

human U6 promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pEGFP VAMP8

Plasmid

#42311

Depositor

Thierry Galli

Publication

Paumet et al J Immunol. 2000 Jun 1;164(11):5850-7.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

VAMP 8 (VAMP8 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

March 12, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pCMV-ZFN-AAVS1

Plasmid

#89707

Purpose

Mammalian expression plasmid for zinc finger nuclease targeting human AAVS1 locus

Depositor

Dmitriy Mazurov

Publication

Zotova et al Viruses. 2017 Nov 2;9(11). pii: v9110325

Insert

ZFN-AAVS1

Expression

Mammalian

Promoter

CMV

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pP3-GFP

Plasmid

#53436

Purpose

Controls GFP expression from the P3 promoter cloned out of S. aureus RN4220 genome

Depositor

Cynthia Collins

Publication

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

P3 promoter

Use

Synthetic Biology

Tags

none

Expression

Bacterial

Mutation

none

Promoter

P3

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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OPEN-HLA-A*11:01-BirA

Plasmid

#235135

Purpose

E. coli expression of BirA tagged HLA-A*11:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

Depositor

Nikolaos Sgourakis

Publication

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN-HLA-A*11:01 (HLA-A Human)

Tags

BirA

Expression

Bacterial

Mutation

G120C

Available since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-Flag-ZO-2

Plasmid

#27420

Depositor

Marius Sudol

Publication

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available since

June 6, 2011

Availability

Academic Institutions and Nonprofits only
SERK2 P5_pECIA14

Plasmid

#114768

Purpose

Prey vector SERK2 P5_pECIA14 should be used with bait vector SERK2 P5_pECIA2.

Depositor

Youssef Belkhadir

Publication

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G34210 (SERK2 Mustard Weed)

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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SERK2 P5_pECIA2

Plasmid

#114968

Purpose

Bait vector SERK2 P5_pECIA2 should be used with prey vector SERK2 P5_pECIA14.

Depositor

Youssef Belkhadir

Publication

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G34210 (SERK2 Mustard Weed)

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HtrA2-FLAG

Plasmid

#15938

Depositor

L. Miguel Martins

Publication

Plun-Favreau et al Nat Cell Biol. 2007 Nov;9(11):1243-52. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HtrA2 (HTRA2 Human)

Tags

FLAG

Expression

Mammalian

Available since

Nov. 5, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMSCV-Myc-Caspase-11_humanized-mutant

Plasmid

#214316

Purpose

Expression of gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Publication

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (Casp4 Mouse)

Use

Retroviral

Tags

Myc

Mutation

KLS288YKT_KASIHS348TPRAKA

Promoter

MESV

Available since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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