Addgene

We narrowed to 21,567 results for: ESC

Showing: 601 - 620 of 21567 results

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pBS-SKII-3XHA-HALO-NAT

Plasmid

#188931

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-HALO-NAT

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-HALO-Hygromycin

Plasmid

#188929

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-HALO-Hygromycin

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-fCLIP-NAT

Plasmid

#188921

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fCLIP-NAT

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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mCyRFP1-mMaroon

Plasmid

#84486

Purpose

Direct FRET pair Fusion mCyRFP1-mMaroon

Depositor

Ryohei Yasuda

Article

Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

mCyRFP1-mMaroon1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-rxRFP

Plasmid

#67830

Purpose

Redox-Sensitive Red Fluorescent Protein

Depositor

Huiwang Ai

Article

Fan et al Anal Chem. 2015 Mar 3;87(5):2802-10. doi

Insert

Redox-Sensitive Red Fluorescent Protein

Use

Tags

His Tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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EF.hICN1

Plasmid

#113715

Purpose

Constitutive expression of hICN1, e.g. for hedgehog-induction. Based on addgene #17623, with CMV.GFP removed.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Insert

None

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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EcYbbN pET15b

Plasmid

#60690

Purpose

Recombinant protein expression of YbbN

Depositor

Mark Wilson

Article

Lin et al J Biol Chem. 2011 Jun 3;286(22):19459-69

Insert

YbbN

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only
pBS-SKII-3XHA-fSNAP-URA

Plasmid

#188917

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fSNAP-URA

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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Kusabira Orange-N1 (KO)

Plasmid

#54793

Purpose

Localization: N1 Cloning Vector, Excitation: 548, Emission: 561

Depositor

Atsushi Miyawaki, Michael Davidson

Article

Karasawa et al Biochem J. 2004 Jul 1. 381(Pt 1):307-12.

Type

Empty backbone

Use

Tags

Kusabira Orange

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pBAD-hsGFP

Plasmid

#64911

Purpose

Encodes a Fluorescent Reporter for Hydrogen Sulfide in E. Coli

Depositor

Huiwang Ai

Article

Chen et al Biochemistry. 2014 Sep 23;53(37):5966-74

Insert

hsGFP

Use

Tags

His Tag

Expression

Bacterial

Mutation

Chromophore tyrosine of the protein mutated to TA…

Promoter

araBAD

Available since

May 20, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-HALO-Phleomycin

Plasmid

#188930

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-HALO-Phleomycin

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-fSNAP-NAT

Plasmid

#188916

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fSNAP-NAT

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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mApple-Profilin-10

Plasmid

#54940

Purpose

Localization: Cytoskeleton, Excitation: 568, Emission: 592

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Profilin

Use

Tags

mApple

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only
pAct1IsGFP-1

Plasmid

#38775

Depositor

Peggy Lemaux

Article

Cho et al Plant Cell Rep. 2000 Nov;19(11):1084-108

Insert

Actin promoter and intron

Use

Plant transformation

Tags

nos 3' terminator and sGFP (S65T)

Expression

Mutation

Promoter

Available since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-pHRFP

Plasmid

#67832

Purpose

pH-Sensitive Red Fluorescent Protein

Depositor

Huiwang Ai

Article

Fan et al Anal Chem. 2015 Mar 3;87(5):2802-10. doi

Insert

pH sensitive red fluorescent protein pHRFP

Use

Tags

His Tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
pBS-SKII-3XHA-fCLIP-URA

Plasmid

#188922

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fCLIP-URA

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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Kusabira Orange-C1 (KO)

Plasmid

#54792

Purpose

Localization: C1 Cloning Vector, Excitation: 548, Emission: 561

Depositor

Atsushi Miyawaki, Michael Davidson

Article

Karasawa et al Biochem J. 2004 Jul 1. 381(Pt 1):307-12.

Type

Empty backbone

Use

Tags

Kusabira Orange

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2014

Availability

Academic Institutions and Nonprofits only
pAG414GPD-FvCYP722C2(pYL878)

Plasmid

#178299

Purpose

Expresses FvCYP722C2 from F. vesca in Saccharomyces cerevisiae. Centromeric TRP, PGPD-FvCYP722C2-TCYC1

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

FvCYP722C2

Use

Tags

Expression

Yeast

Mutation

Promoter

GAP

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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mEmerald-LC-Myosin-C-10

Plasmid

#54145

Purpose

Localization: Light Chain Myosin, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

LC-Myosin

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pBS-SKII-URA-GAL1-fCLIP-GS

Plasmid

#188939

Purpose

Integration cassette to create N-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

URA-GAL1-fCLIP-GS

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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