We narrowed to 1,456 results for: U6 promoter

1. px330_SMAD exon 2 gRNA

   Plasmid

   #68350

   Purpose

   px330 with gRNA towards SMAD exon 2. Cas9 is expressed from a CAG promoter.

   Depositor

   Jannik Elverløv-Jakobsen

   Insert

   SMAD exon 2 gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. px330_P53 exon 8 gRNA

   Plasmid

   #68349

   Purpose

   px330 with gRNA towards P53 exon 8. Cas9 is expressed from a CAG promoter.

   Depositor

   Jannik Elverløv-Jakobsen

   Insert

   P53 exon 8 gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. px330_P53 exon 7 gRNA

   Plasmid

   #68351

   Purpose

   px330 with gRNA towards P53 exon 7. Cas9 is expressed from a CAG promoter.

   Depositor

   Jannik Elverløv-Jakobsen

   Insert

   P53 exon 7 gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. px330_PTEN exon 5 gRNA

   Plasmid

   #68348

   Purpose

   px330 with gRNA towards PTEN exon 5. Cas9 is expressed from a CAG promoter.

   Depositor

   Jannik Elverløv-Jakobsen

   Insert

   PTEN exon 5 gRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV-dCas13X.1-RESCUE-S-SV40pA_U6-BbsI-DR_CMV-mCherry-P2A-Puro-BGHpA

   Plasmid

   #171384

   Purpose

   Expression vector for encoding a human codon-optimized dCas13X.1-RESCUE-S driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

   Depositor

   Hui Yang

   Article

   Xu et al Nat Methods. 2021 May;18(5):499-506. doi

   Insert

   humanized dCas13X.1-RESCUE-S
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, U6

   Available since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. CMV-minidCas13X.1-RESCUE-S-SV40pA_U6-BbsI-DR_CMV-mCherry-P2A-Puro-BGHpA

   Plasmid

   #171385

   Purpose

   Expression vector for encoding a human codon-optimized minidCas13X.1-RESCUE-S driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

   Depositor

   Hui Yang

   Article

   Xu et al Nat Methods. 2021 May;18(5):499-506. doi

   Insert

   humanized minidCas13X.1-RESCUE-S
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, U6

   Available since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV KI Cloning Vector_hSyn mTagBFP2-CAAX2

   Plasmid

   #236245

   Purpose

   Cloning template for knock-in based strategy with U6 promoter, gRNA and donor tag cloning cassettes, and a fluorescent marker for the plasma membrane under hSyn promoter

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   mTagBFP2 fused to the plasma membrane targeting sequence CAAX2
   

   Use

   AAV

   Tags

   mTagBFP2

   Expression

   Mutation

   Promoter

   U6 and human Synapsin1

   Available since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRB17

   Plasmid

   #52527

   Purpose

   serves as template for PCR-mediated fusion of U6-promoter with sgRNA

   Depositor

   Klaus Foerstemann

   Article

   Bottcher et al Nucleic Acids Res. 2014 Apr 19.

   Insert

   Drosophila U6C promoter
   

   Use

   CRISPR; Blunt cloning kit

   Tags

   T7-promoter

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. CIRTS-control

   Plasmid

   #213788

   Purpose

   CIRTS RNA targeting system for targeted knockdown in neurons. CIRTS-Calm3 and scramble control guide RNA is expressed under human Syn1 and U6 promoter, respectively.

   Depositor

   Timothy Bredy

   Article

   Liau et al Nat Commun. 2023 Nov 22;14(1):7616. doi:

   Insert

   CIRTS-Calm3-U6-control gRNA
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Syn1, U6

   Available since

   Feb. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. CIRTS-FTO-control

    Plasmid

    #216859

    Purpose

    CIRTS RNA targeting system for targeted removal of m6A on transcript at the synapse. CIRTS-FTO-Calm3 and scramble gRNA is expressed under human Syn1 and U6 promoter, respectively.

    Depositor

    Timothy Bredy

    Article

    Madugalle et al J Neurosci. 2023 Oct 25;43(43):7084-7100

    Insert

    CIRTS-FTO-Calm3-U6-control gRNA
    

    Use

    Lentiviral

    Tags

    mCherry2

    Expression

    Mammalian

    Mutation

    Promoter

    Syn1, U6

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. CIRTS-Gas5

    Plasmid

    #213172

    Purpose

    CIRTS RNA targeting system for targeted knockdown in neurons. CIRTS-Calm3 and Gas5 guide RNA is expressed under human Syn1 and U6 promoter, respectively.

    Depositor

    Timothy Bredy

    Article

    Liau et al Nat Commun. 2023 Nov 22;14(1):7616. doi:

    Insert

    CIRTS-Calm3-U6-Gas5 gRNA
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Syn1, U6

    Available since

    Feb. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. CIRTS-FTO-Malat1

    Plasmid

    #216860

    Purpose

    CIRTS RNA targeting system for targeted removal of m6A on Malat1 at the synapse. CIRTS-FTO-Calm3 and Malat1 gRNA is expressed under human Syn1 and U6 promoter, respectively.

    Depositor

    Timothy Bredy

    Article

    Madugalle et al J Neurosci. 2023 Oct 25;43(43):7084-7100

    Insert

    CIRTS-FTO-Calm3-U6-Malat1 gRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Syn1, U6

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. OpenCRISPR-1 sgRNA-16nt stem

    Plasmid

    #221566

    Purpose

    Express OpenCRISPR-1 sgRNA (16nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.

    Depositor

    Ali Madani

    Article

    Ruffolo et al Nature. 2025 Jul 30. doi: 10.1038/s41586

    Insert

    OpenCRISPR-1 sgRNA 16nt stem loop
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 28, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. OpenCRISPR-1 sgRNA-12nt stem

    Plasmid

    #221567

    Purpose

    Express OpenCRISPR-1 sgRNA (12nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.

    Depositor

    Ali Madani

    Article

    Ruffolo et al Nature. 2025 Jul 30. doi: 10.1038/s41586

    Insert

    OpenCRISPR-1 sgRNA 12nt stem loop
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 28, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. eSpCas9(1.1)_No_FLAG_NPM1_G6

    Plasmid

    #178091

    Purpose

    Expresses the NPM1 G6 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with NPM1_mNeonGreen_Donor to tag NPM1 with mNeonGreen. pX330-like plasmid.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    NPM1 G6 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEJS887_pTetR-P2A-BFPnls/sgAlpha

    Plasmid

    #108651

    Purpose

    Alpha satellite repeats targeting Spy sgRNA under U6 promoter

    Depositor

    Erik Sontheimer

    Article

    Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

    Inserts

    Alpha satellite repeats targeting sgRNA
    TetR-P2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    No

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and hPGK

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. eSpCas9(1.1)_No_FLAG_LMNA_G2

    Plasmid

    #178090

    Purpose

    Expresses the LMNA G2 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with LMNA_mScarlet-I_Donor to tag LMNA with mScarlet-I. pX330-like plasmid.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    LMNA G2 sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pU6-mir144~451 EF1Alpha-puro-T2A-BFP

    Plasmid

    #164793

    Purpose

    Express miR-144~451 under the U6 promoter in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    miR-144~451 (MIR451A, MIR144 Human)
    puro-T2A-BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1Alpha and U6

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPN062

    Plasmid

    #91595

    Purpose

    Express sgRNA targeting human DFNA5

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    DFNA5 (GSDME Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPN063

    Plasmid

    #91596

    Purpose

    Express sgRNA targeting human DFNA5

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    DFNA5 (GSDME Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge