Addgene

We narrowed to 4,219 results for: biorxiv

Showing: 601 - 620 of 4219 results

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pAPInt-mScarlet-Hyg

Plasmid

#236492

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mScarlet and selection on Hyg.

Depositor

Daniel Lew

Article

Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

Insert

mScarlet

Use

Tags

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Promoter

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-GFP-Hyg

Plasmid

#236477

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with GFP and selection on Hyg.

Depositor

Daniel Lew

Article

Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Promoter

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-sfGFP-Nat

Plasmid

#236479

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with sfGFP and selection on Nat.

Depositor

Daniel Lew

Article

Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

Insert

sfGFP

Use

Tags

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Promoter

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-mNeonGreen-Nat

Plasmid

#236482

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mNeonGreen and selection on Nat.

Depositor

Daniel Lew

Article

Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

Insert

mNeonGreen

Use

Tags

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Promoter

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-GFP-G418

Plasmid

#236478

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with GFP and selection on G418.

Depositor

Daniel Lew

Article

Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Promoter

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pHACK-Sparse.FRT10-GAL4.DBD

Plasmid

#232833

Purpose

Sparse.FRT10 GAL4.DBD for HACK system

Depositor

Liqun Luo

Article

Xu et al bioRxiv [Preprint]. 2024 Dec 4:2024.12.0

Inserts

FRT10-STOP-FRT10
GAL4.DBD (GAL4 Budding Yeast)

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pHACK-Sparse.FRT10-GAL4

Plasmid

#232834

Purpose

Sparse.FRT10 GAL4 for HACK system

Depositor

Liqun Luo

Article

Xu et al bioRxiv [Preprint]. 2024 Dec 4:2024.12.0

Inserts

FRT10-STOP-FRT10
GAL4 (GAL4 Budding Yeast)

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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VT028327-FRT10-STOP-FRT10-p65.AD

Plasmid

#232827

Purpose

Enhancer VT028327 driven p65.AD, gated by Sparse.FRT10

Depositor

Liqun Luo

Article

Xu et al bioRxiv [Preprint]. 2024 Dec 4:2024.12.0

Inserts

FRT10-STOP-FRT10 (stop )
p65.AD

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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VT028327-FRT10-STOP-FRT10-GAL4

Plasmid

#232829

Purpose

Enhancer VT028327 driven GAL4, gated by Sparse.FRT10

Depositor

Liqun Luo

Article

Xu et al bioRxiv [Preprint]. 2024 Dec 4:2024.12.0

Inserts

FRT10-STOP-FRT10
GAL4 (GAL4 Budding Yeast)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pBP-Sparse.FRT10-GAL4.DBD

Plasmid

#232831

Purpose

Enhancer-free Sparse.FRT10 GAL4.DBD with ccdB for Gateway

Depositor

Liqun Luo

Article

Xu et al bioRxiv [Preprint]. 2024 Dec 4:2024.12.0

Inserts

FRT10-STOP-FRT10
GAL4.DBD (GAL4 Budding Yeast)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pPB_EF1a_Gag

Plasmid

#212655

Purpose

piggyBac vector to express MLV Gag

Depositor

Paul Blainey

Article

Najia et al bioRxiv 2024.10.01.616098

Insert

MLV Gag (UniProt ID: P03332) (gag MoMLV)

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pRDB_092

Plasmid

#231110

Purpose

C>T base editor

Depositor

David Root, John Doench

Insert

TadA-CDd-nCas9-SpG-UGI

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

D10A

Promoter

Available since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pX330_REC8_cterm

Plasmid

#222522

Purpose

Cas9/sgRNA plasmid for targeting REC8

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

Cas9, REC8 sgRNA (REC8 Human, Synthetic)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pHDR2_mGreenLantern-T2A-SYCP3

Plasmid

#222523

Purpose

Homology directed repair template for knocking in mGreenLantern reporter to SYCP3.

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

mGreenLantern

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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AA516

Plasmid

#231118

Purpose

Fragmid fragment: (2A Selection) CBE activity-based selection intron

Depositor

David Root, John Doench

Insert

PuroN'-intron-PuroC'

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGH896

Plasmid

#230840

Purpose

Ef1a-DivA-BE-T2A-tagBFP-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al bioRxiv [Preprint]. 2024 Nov 19:2024.11.

Insert

DivA-BE

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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AA498

Plasmid

#231119

Purpose

Fragmid fragment: (guide cassette) ABE activity-based selection CD274 positive control

Depositor

David Root, John Doench

Insert

sgCD274 + ABE splice-targeting sgRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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AA505

Plasmid

#231120

Purpose

Fragmid fragment: (guide cassette) CBE activity-based selection CD274 positive control

Depositor

David Root, John Doench

Insert

sgCD274 + CBE splice-targeting sgRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGH941

Plasmid

#230846

Purpose

Ef1a-DivA-BE-T2A-GFP-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al bioRxiv [Preprint]. 2024 Nov 19:2024.11.

Insert

DivA-BE

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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AA509

Plasmid

#231116

Purpose

Fragmid fragment: (2A Selection) ABE activity-based selection intron

Depositor

David Root, John Doench

Insert

PuroN'-intron-PuroC'

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge