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plenti-UbcP-HA-CRBN-pGK-HYG

Plasmid

#107378

Purpose

Lentiviral expression of HA-CRBN

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

cereblon (CRBN Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

UbcP

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pFRT-DestFLAGHA_RBPMS

Plasmid

#65743

Purpose

constitutively expresses RBPMS in mammalian cells

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Use

Tags

FLAG HA

Expression

Mammalian

Mutation

Promoter

CMV, 2x Tet

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only
pFRT-DestFLAGHA_RBPMS2

Plasmid

#65748

Purpose

constitutively expresses FLAG-HA-tagged RBPMS2 in mammalian cells

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS2 (RBPMS2 Human)

Use

Tags

FLAG HA

Expression

Mammalian

Mutation

Promoter

CMV, 2x Tet

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only
pUC18T-mini-Tn7T-Gm-Pc-sfGFP

Plasmid

#199248

Purpose

Fluorescent labelling of bacterial cells with sfGFP

Depositor

Uwe Mamat

Article

Mamat et al Appl Environ Microbiol. 2023 May 17:e003

Insert

Codon-optimized sfGFP gene

Use

Tags

Expression

Bacterial

Mutation

Codon optimization for Xanthomonadaceae

Promoter

Pc promoter from class III integron of Delftia a…

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX FLAG-SLC38A9.1

Plasmid

#199749

Purpose

Lentiviral construct to stably express SLC38A91.1

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

SLC38A9.1

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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plenti-UBC- IKZF4-3xHA-pGK-PUR

Plasmid

#107384

Purpose

Lentiviral expression of IKZF4-3xHA

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF4 (IKZF4 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

UBC

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pBp-FGFR2b-WT

Plasmid

#45698

Depositor

Matthew Meyerson

Article

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR2 IIIb (FGFR2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only
pNoROS-CAT

Plasmid

#170152

Purpose

Mammalian expression vector encoding a constitutive nucleolar targeted roGFP and a doxycycline inducible nucleolar targeted catalase. Used to study nucleolar oxidation

Depositor

Dimitri Pestov

Article

Sapio et al Front Mol Biosci. 2021 Apr 26;8:678488.

Inserts

catalase (Cat Mouse)
roGFP2

Use

Genome integration via sleeping beauty transposon…

Tags

FLAG tag and Nucleolar Localization Sequence (NoL…

Expression

Mammalian

Mutation

Promoter

RPBSA and TRE promoter

Available since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3-hALDOA

Plasmid

#210809

Purpose

For transient transfection

Depositor

Sheng-cai Lin

Article

Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

Insert

ALDOA (ALDOA Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pShuttle CMV RGECO-TnT

Plasmid

#124643

Purpose

Myofilament localised red genetically encoded calcium sensor in mammalian expression vector used for AdEasy adenoviral DNA recombination.

Depositor

Paul Robinson, Matthew Daniels, Hugh Watkins

Article

Sparrow et al Circ Res. 2019 Feb 8. doi: 10.1161/CIRCR

Insert

RGECO-TnT (TNNT2 Synthetic, Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX neo PFKL-GFP

Plasmid

#138289

Purpose

Mammalian Expression, Lentiviral. Expression of human phosphofructokinase, liver isoform PFKL-GFP fusion protein

Depositor

Gaudenz Danuser

Article

Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

Insert

PFKL (PFKL Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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FLAG-MED18

Plasmid

#15419

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED18 (MED18 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
MSCV-mir-17-92

Plasmid

#64100

Purpose

expresses mir-17-92

Depositor

Lin He

Article

Olive et al Elife. 2013 Oct 15;2:e00822. doi: 10.755

Insert

miR-17-92 (MIR17HG Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

MSCV

Available since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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U6-pegRNA-H1-nick sgRNA-mCherry

Plasmid

#176901

Purpose

Expresses prime editing guide RNA and guide RNA in mammalian cells

Depositor

Hyongbum Kim

Article

Jang et al Nat Biomed Eng. 2021 Aug 26. pii: 10.103

Inserts

mCherry
gRNA scaffold
gRNA scaffold

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG, H1, and U6

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-IKZF2-V2-V5

Plasmid

#107391

Purpose

Mammalian expression of IKZF2-V2-V5

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF2-V2 (IKZF2 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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MSCVpic2neo-hTERT-p16 shRNA

Plasmid

#164920

Purpose

Expresses hTERT cDNA and p16 shRNA in mammalian cells

Depositor

Ji Luo

Article

Smith et al Cell Biosci. 2016 Nov 11;6:57. doi: 10.1

Inserts

human telomerase reverse transcriptase (TERT Human)
CDKN2A (CDKN2A Human)

Use

Retroviral

Tags

HA tag

Expression

Mutation

Promoter

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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HyperADAR control

Plasmid

#166969

Purpose

Express the Hyperactive catalytic domain of Drosophila ADAR and linker at the 5' end of this domain, in the MSCV-IRES-GFP

Depositor

Michael Kharas

Article

Nguyen et al Nat Commun. 2020 Apr 24;11(1):2026. doi:

Insert

linker-hyperADAR

Use

Retroviral

Tags

linker-HyperADAR

Expression

Mammalian

Mutation

Promoter

MSCV

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-Gm-Pc-tdTomato

Plasmid

#199250

Purpose

Fluorescent labelling of bacterial cells with tdTomato

Depositor

Uwe Mamat

Article

Mamat et al Appl Environ Microbiol. 2023 May 17:e003

Insert

Codon-optimized tdTomato gene

Use

Tags

Expression

Bacterial

Mutation

Codon optimization for Xanthomonadaceae

Promoter

Pc promoter from class III integron of Delftia a…

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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1179_pAAV-U6-BbsI-gRNA-CB-EmGFP

Plasmid

#89060

Purpose

AAV-gRNA cloning vector with GFP reporter

Depositor

William Lagor

Article

Jarrett et al Sci Rep. 2017 Mar 16;7:44624. doi: 10.10

Insert

EmGFP

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Chicken beta actin with partial CMV enhancer

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pAC94-pmax-dCas9VP160-2A-puro

Plasmid

#48226

Purpose

dCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note: This is being tested.

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-puro

Use

CRISPR

Tags

2A-puro, HA Tag, and VP160

Expression

Mammalian

Mutation

D10A;H840A (catalytically inactive)

Promoter

CAGGS

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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