We narrowed to 13,978 results for: eng

Showing: 601 - 620 of 13978 results

Results per page:

Sort results by:

ABE9

Plasmid

#194208

Purpose

Mammalian expression of ABE9

Depositor

Dali Li

Article

Chen et al Nat Chem Biol. 2022 Oct 13. pii: 10.1038

Insert

TadA-8e (N108Q/L145T)-nSpCas9

Use

CRISPR

Tags

6*His

Expression

Mammalian

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
P021-mPB-ENGRAM-All-in-one-PGK-BFP-P2A-Blast

Plasmid

#239972

Purpose

Updated ENGRAM plasmid that is ready for both 5' and 3'-FT ENGRAM cloning

Depositor

Jay Shendure

Article

Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3-FLAG-Regnase-3-HA_full-length

Plasmid

#222656

Purpose

Mammalian expression vector to express full-length Regnase-3 tagged with FLAG at N-term and HA at C-term

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c (Zc3h12c Mouse)

Tags

FLAG and HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Beta1 Q280* K418* full-length

Plasmid

#221406

Purpose

Mammalian expression of human integrin beta1 Q280* K418* full-length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 Q280* K418* full-length (ITGB1 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Beta1 N391* N562* full-length

Plasmid

#221407

Purpose

Mammalian expression of human integrin beta1 N391* N562* full-length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin beta1 N391* N562* full-length (ITGB1 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and HA …

Expression

Mammalian

Mutation

codon-optimized mature sequence

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Fusion of Dengue2 NS2B-NS3 proteins

Plasmid

#204793

Purpose

Bacterial expression of a fusion of Dengue2 NS2B-NS3 proteins

Depositor

ASAP Discovery, Frank von Delft

Insert

Dengue2 NS2B-NS3 fusion

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCMV(WT)-mouse-full-length-CHMP3-HA

Plasmid

#154175

Purpose

expresses mouse CHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3 (Chmp3 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDB327

Plasmid

#113671

Purpose

expresses Ulp1_R3 in Escherichia coli

Depositor

Christopher Bahl

Article

Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

Insert

Ulp1_R3

Tags

6-His

Expression

Bacterial

Mutation

contains solubility enhancing mutations

Promoter

T7

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pKM496 (deletions) Zeo

Plasmid

#109301

Purpose

ORBIT integrating plasmid for deletion of target gene, using zeocin as a selection marker.

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

Bxb1 attB site

Use

Orbit integrating plasmid

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJRH-1179 U6-reci Gag-Cas9 v2

Plasmid

#201914

Purpose

Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

Gag-Cas9 v2

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
gag-COM-pol

Plasmid

#211373

Purpose

Packaging plasmid for v3b PE-eVLP production

Depositor

David Liu

Article

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

MMLVgag-COM-pol

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
gag-P3-pol

Plasmid

#211374

Purpose

Packaging plasmid for v3b PE-eVLP production

Depositor

David Liu

Article

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

MMLVgag-P3-pol

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pm-iATPSnFR1.0

Plasmid

#102548

Purpose

membrane displayed ATP sensor for mammalian cells

Depositor

Baljit Khakh

Article

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

iATPSnFR1.0

Expression

Mammalian

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-Ftractin-mCherry

Plasmid

#85131

Purpose

lentiviral expression of an F-actin live-cell marker (mCherry)

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

Ftractin-mCherry (amino acids 9-52 of rat ITPKA, C-temrinally tagged with mCherry)

Use

Lentiviral

Tags

mCherry

Promoter

EF1a

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
NGFR

Plasmid

#27489

Depositor

Warren Pear

Article

Izon et al Immunity. 2001 Mar . 14(3):253-64.

Type

Empty backbone

Use

Retroviral

Tags

ECMV, IRES, and NGFR

Expression

Mammalian

Available Since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pINDUCER21-MYB

Plasmid

#51305

Purpose

Expression of MYB after doxycycline administration

Depositor

George Daley

Article

Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

Insert

v-myb avian myeloblastosis viral oncogene homolog (MYB Human)

Use

Lentiviral

Tags

n/a

Mutation

full length ORF

Available Since

June 13, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-PE2-BSD

Plasmid

#161514

Purpose

Expression of PE2 in mammalian cells. Can be used to generate stable cell lines by Blasticidin selection

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2020 Sep 21. pii: 10.103

Insert

PE2-P2A-BSD

Use

Lentiviral

Tags

SV40 NLS

Expression

Mammalian

Promoter

EF-1α core promoter

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pWJ66

Plasmid

#46570

Purpose

Same as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)

Depositor

Luciano Marraffini

Article

Bikard et al Nucleic Acids Res. 2013 Jun 12.

Inserts

tracrRNA
dcas9-w
CRISPR array

Use

CRISPR

Tags

rpoZ

Expression

Bacterial

Mutation

D10A, H840A

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAVS1-Nst-MCS

Plasmid

#80487

Purpose

donor vector for AAVS1 targeting (neomycin selection) with multiple cloning site

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Type

Empty backbone

Use

Donor vector (human)

Expression

Mammalian

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDIRECT_23C

Plasmid

#91140

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:bar

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers

Use

CRISPR

Expression

Plant

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge