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pFastBac-LacI-LAP-HEC1

Plasmid

#114033

Purpose

Production of baculovirus with LacI-LAP-HEC1

Depositor

Geert Kops

Article

Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

Insert

LacI-LAP-HEC1 (NDC80 Human)

Use

Tags

Expression

Insect

Mutation

none

Promoter

CMV

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P3nmt1-mCFP

Plasmid

#105136

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

kanMX6-P3nmt1-mCFP

Use

Tags

3nmt1-mCFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

3nmt1

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P3nmt1-mECitrine

Plasmid

#105151

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Ye et al Mol Biol Cell. 2012 Apr;23(7):1181-95. d

Insert

kanMX6-P3nmt1-mECitrine

Use

Tags

3nmt1-mECitrine

Expression

Bacterial and Yeast

Mutation

F64L, Q69M, A206K

Promoter

3nmt1

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P3nmt1-mYFP

Plasmid

#105138

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

kanMX6-P3nmt1-mYFP

Use

Tags

3nmt1-mYFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

3nmt1

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-3nmt1-mEOS3.2

Plasmid

#105166

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Liu et al Mol Biol Cell. 2016 Aug 15;27(16):2528-4

Insert

kanMX6-3nmt1-mEOS3.2

Use

Tags

3nmt1-mEOS3.2

Expression

Bacterial and Yeast

Mutation

Promoter

3nmt1

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P81nmt1-mEGFP

Plasmid

#105143

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Laporte et al Mol Biol Cell. 2012 Aug;23(16):3094-110.

Insert

kanMX6-P81nmt1-mEGFP

Use

Tags

81nmt1-mEGFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

81nmt1

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P81nmt1-mECitrine

Plasmid

#105153

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Davidson et al PLoS One. 2016 Nov 29;11(11):e0167043. d

Insert

kanMX6-P81nmt1-mECitrine

Use

Tags

81nmt1-mECitrine

Expression

Bacterial and Yeast

Mutation

F64L, Q69M, A206K

Promoter

81nmt1

Available since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P41nmt1-mECitrine

Plasmid

#105152

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Wang et al J Cell Biol. 2014 May 12;205(3):357-75.

Insert

kanMX6-P41nmt1-mECitrine

Use

Tags

41nmt1-mECitrine

Expression

Bacterial and Yeast

Mutation

F64L, Q69M, A206K

Promoter

41nmt1

Available since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac-ATG101-ATG13 HORMA

Plasmid

#99332

Purpose

Mammalian expression of one subcomplex from the Human ULK1 complex

Depositor

James Hurley

Article

Qi et al Structure. 2015 Oct 6;23(10):1848-57. do

Inserts

ATG101 (ATG101 Human)
ATG13 (ATG13 Human)

Use

Tags

GST

Expression

Insect

Mutation

Promoter

p10 and pH

Available since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pFA6a-pcr1P-natMX6-pcr2P

Plasmid

#80620

Purpose

to delete part of the mat1-P locus and fix the mating type in natural S. pombe isolates

Depositor

Miguel Godinho Ferreira

Article

Avelar et al Nat Commun. 2013;4:2235. doi: 10.1038/nc

Inserts

PCR1 from mat1-P
PCR2 from mat1-P

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pFA6a-pcr1M-natMX6-pcr2M

Plasmid

#80621

Purpose

to delete part of the mat1-M locus and fix the mating type in natural S. pombe isolates

Depositor

Miguel Godinho Ferreira

Article

Avelar et al Nat Commun. 2013;4:2235. doi: 10.1038/nc

Inserts

PCR1 from mat1-M
PCR2 from mat1-M

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pFA6a-VN155(I153L)-TRP1

Plasmid

#53193

Purpose

chromosomal tagging with Venus

Depositor

John Pringle

Article

Onishi et al J Cell Biol. 2013 Jul 22;202(2):311-29.

Insert

Venus

Use

Yeast genomic targeting

Tags

TRP1

Expression

Mutation

aa 1–155, I to L mutation at aa 153

Promoter

Available since

May 7, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomEmerald-CaURA3

Plasmid

#44890

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mEmerald with CaUra3 selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomEmerald

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-PhiYellow-SpHis5

Plasmid

#44651

Purpose

Yeast tagging vector to create gene fusion with PhiYellow with SpHis5 selection

Depositor

Kurt Thorn

Insert

phiYellow

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoMaxGFP-CaURA3

Plasmid

#44875

Purpose

Yeast tagging vector to create gene fusion with yeast optimized MaxGFP with CaUra3 selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoMaxGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoMaxGFP-SpHis5

Plasmid

#44840

Purpose

Yeast tagging vector to create gene fusion with yeast optimized MaxGFP with SpHis5 selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoMaxGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-His3MX6-PGAL1-GST

Plasmid

#41613

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

A. gossypii translation elongation factor 1a gene…

Expression

Mutation

Promoter

Available since

Dec. 11, 2012

Availability

Academic Institutions and Nonprofits only

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pFastBac(His10-Lckg2a,Y394F)

Plasmid

#177893

Purpose

Baculo expression of His10 tag fused to human LckG2A

Depositor

Ron Vale

Article

Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

Insert

Lck (LCK Human)

Use

Tags

10xHis-TEV

Expression

Insect

Mutation

G2A,Y394F

Promoter

Polyhedrin

Available since

Availability

Academic Institutions and Nonprofits only

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pFA6a-TurboID-3MYC-natMX6 (pFB1434)

Plasmid

#126050

Purpose

For C-terminal tagging with TurboID in yeast including nourseothricin-resistant marker

Depositor

François Bachand

Article

Larochelle et al J Cell Sci. 2019 May 7. pii: jcs.232249.

Insert

TurboID

Use

Pcr-based c-terminal tagging

Tags

Expression

Mutation

Promoter

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBac Dual α1B/β2B Tubulin

Plasmid

#170553

Purpose

Encodes codon-optimized human α1B and β2B tubulin for expression in and purification from insect cells.

Depositor

Tarun Kapoor

Article

Ti et al Dev Cell. 2018 Oct 22;47(2):175-190.e5.

Inserts

TUBA1B (TUBA1B Human)
TUBB2B (TUBB2B Human)

Use

Tags

AP linker, GS linker (GGSGG), His10 tag, L21 enha…

Expression

Insect

Mutation

Promoter

Available since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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