We narrowed to 6,573 results for: tac

1. pTwist-SARS-CoV-2 Spike-deltaC18 T307I (ATA) T572I (ATC) D614G (GGT)

   Plasmid

   #212377

   Purpose

   Encodes SARS-CoV-2 spike T307I, T572I, D614G for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike T307I, T572I, D614G (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T307I, T572I, D614G

   Promoter

   CAG

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTwist-SARS-CoV-2 Spike-deltaC18 D614G (GGT) Q677H (CAT) D936Y (TAT)

   Plasmid

   #212379

   Purpose

   Encodes SARS-CoV-2 spike D614G, Q677H, D936Y for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike D614G, Q677H, D936Y (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D614G, Q677H, D936Y

   Promoter

   CAG

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTwist-SARS-CoV-2 Spike-deltaC18 SAK006840 VOC-501Y.V2 (B.1.351v3) - No RBD Mutations

   Plasmid

   #212380

   Purpose

   Encodes SARS-CoV-2 spike D80A, deltaL242-L244, R246I, D614G, A701V for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike D80A, G215D, deltaL242-L244, R246I, D614G, A701V (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D80A, G215D, deltaL242-L244, R246I, D614G, A701V

   Promoter

   CAG

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCL051 AAVS1-9xTetO-nTATAchr21-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

   Plasmid

   #188745

   Purpose

   Donor vector for integrating nonTATAchr21 promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Surface marker and mCitrine reporter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   nonTATAchr21

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCL053 AAVS1-9xTetO-nTATAchrX-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

   Plasmid

   #188746

   Purpose

   Donor vector for integrating nonTATAchrX promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Surface marker and mCitrine reporter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   nonTATAchrX

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTwist-SARS-CoV-2 Spike-deltaC18 S371F (TTC) S373P (CCA) S375F (TTC) T376A (GCT)

   Plasmid

   #212493

   Purpose

   Encodes SARS-CoV-2 spike S371F, S373P, S375F, T376A for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike S371F, S373P, S375F, T376A (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S371F, S373P, S375F, T376A

   Promoter

   CAG

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTwist-SARS-CoV-2 Spike-deltaC18 Q493R (CGA) Q498R (CGA) N501Y (TAT) Y505H (CAT)

   Plasmid

   #212498

   Purpose

   Encodes SARS-CoV-2 spike Q493R, Q498R, N501Y, Y505H for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike Q493R, Q498R, N501Y, Y505H (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q493R, Q498R, N501Y, Y505H

   Promoter

   CAG

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTwist-SARS-CoV-2 Spike-deltaC18 K417N (AAT) L452R (CGT) T478K (AAA) E484K (AAA)

   Plasmid

   #212439

   Purpose

   Encodes SARS-CoV-2 spike K417N, L452R, T478K, E484K for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike K417N, L452R, T478K, E484K (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K417N, L452R, T478K, E484K

   Promoter

   CAG

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTwist-SARS-CoV-2 Spike-deltaC18 Q493R (CGA) G496S (TCA) Q498R (CGA) N501Y (TAT) Y505H (CAT)

   Plasmid

   #212477

   Purpose

   Encodes SARS-CoV-2 spike Q493R, G496S, Q498R, N501Y, Y505H for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike Q493R, G496S, Q498R, N501Y, Y505H (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q493R, G496S, Q498R, N501Y, Y505H

   Promoter

   CAG

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTRC-LIC

    Plasmid

    #62343

    Purpose

    Used for tac promoter regulated high-level expression of cloned, untagged genes in E. Coli.

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter from pKK233-2

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ST-2xTstem(5A)-GFP

    Plasmid

    #162504

    Purpose

    Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. One wildtype Tac stem region is inserted within ST and the other mutated Tac stem region is inserted between ST and GFP.

    Depositor

    Lei Lu

    Article

    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

    Insert

    GFP tagged ST6Gal1 mutant (ST6GAL1, IL2RA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    One Tac stem region is inserted within ST6Gal1 an…

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ST-2xTstem-GFP

    Plasmid

    #162503

    Purpose

    Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. One Tac stem region is inserted within ST and the other Tac stem region is inserted between ST and GFP.

    Depositor

    Lei Lu

    Article

    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

    Insert

    GFP tagged ST6Gal1 mutant (ST6GAL1, IL2RA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    One Tac stem region is inserted within ST6Gal1 an…

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ST-Tstem(5A)-GFP

    Plasmid

    #162502

    Purpose

    Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. The mutated stem region of Tac is inserted within ST.

    Depositor

    Lei Lu

    Article

    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

    Insert

    ST6Gal1 mutant (the mutated Tac stem region is inserted within ST) (ST6GAL1, IL2RA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    The mutated Tac stem region is inserted within ST…

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ST-Tstem-GFP

    Plasmid

    #162501

    Purpose

    Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. The stem region of Tac is inserted within ST.

    Depositor

    Lei Lu

    Article

    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

    Insert

    ST6Gal1 mutant (Tac stem region is inserted within ST) (ST6GAL1, IL2RA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    The Tac stem region is inserted within ST6Gal1

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSEVA47_Helper

    Plasmid

    #231420

    Purpose

    M13 helper genes cloned into the pSEVA631 vector. This plasmid does not carry an M13 packaging signal. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ296.)

    Depositor

    Alfonso Jaramillo

    Article

    Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

    Insert

    gp1-gp11
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJB37

    Plasmid

    #202602

    Purpose

    Arabinose inducible expression vector expressing retron Rcat-Sen2

    Depositor

    Athanasios Typas

    Article

    Bobonis et al Nat Protoc. 2024 May 9. doi: 10.1038/s41

    Insert

    RcaT-Sen2 toxin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSEVA63_sgRNA-B1_M13ps

    Plasmid

    #231422

    Purpose

    M13 phagemid that expresses sgRNA-B1 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ615.)

    Depositor

    Alfonso Jaramillo

    Article

    Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

    Insert

    sgRNA-B1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSEVA47_dCas9-GFP_NOT_iC

    Plasmid

    #231418

    Purpose

    This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-C1. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ290.)

    Depositor

    Alfonso Jaramillo

    Article

    Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

    Inserts

    dCas9
    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pST

    Plasmid

    #213143

    Purpose

    TtgV Biosensor

    Depositor

    Radhakrishnan Mahadevan

    Article

    Pham et al ACS Synth Biol. 2024 Jul 19;13(7):2246-2

    Insert

    TtgV sfGFP Biosensor
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSEVA26_sgRNA-2_M13ps

    Plasmid

    #231412

    Purpose

    M13 phagemid that expresses sgRNA-2 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ171.)

    Depositor

    Alfonso Jaramillo

    Article

    Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

    Insert

    sgRNA-2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge