172,546 results
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Plasmid#25874DepositorInsertEYFP H148Q/I152L
ExpressionMammalianMutationEYFP from pEYFP (Clontech) with H148Q and I152L m…Available SinceAug. 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFH2.12_GagZip-MCP-P2A-eGFP
Plasmid#205527PurposeExpress HIV GagZip-MCP-P2A-eGFPDepositorInsertGagZip-MCP
ExpressionMammalianMutationNucleocapsid replaced with leucine zipperPromoterCMVAvailable SinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET47b-T7-AsCas12f-WT
Plasmid#204640PurposeExpression of wild-type AsCas12f in E. coliDepositorInsertAsCas12f
Tags6xHisExpressionBacterialPromoterT7Available SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-PEmax-P2A-GFP
Plasmid#202000PurposeBased on the pCMV-PEmax-P2A-GFP, we replaced the CMP promoter with the EF1a promoter for expression in iPSC.DepositorInsertPEmax-P2A-GFP
UseCRISPR and Synthetic BiologyTagsGFPExpressionMammalianPromoterEF1aAvailable SinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
EF1a-Cas9-2XNES-2ERT2
Plasmid#120551PurposeExpress Cas9, 2xNES and 2 tandem ERT2 in mammalian cellsDepositorInsertCas9, NES and ERT2
UseLentiviralExpressionMammalianPromoterEF1αAvailable SinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
LV-indLS2
Plasmid#123068PurposeLentiviral construct that delivers a tamoxifen inducible Cre. Upon recombination, luc2 and mStrawberry express. Used to image spontaneous tumorigenesis or subclonal cell populations in vivo.DepositorInsertsCreERT2 with intron
Firefly luciferase
mStrawberry
UseLentiviral and Luciferase; FluorescenceExpressionMammalianMutationsynthetic intron added as indicatedPromoterCAGGS (after Cre mediated inversion) and CAGGS (f…Available SinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET24a-ncOGT-FL
Plasmid#190821PurposeFor expression of full-length human ncOGT in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.DepositorInsertO-GlcNAc Transferase (OGT Human)
Tags6x HisExpressionBacterialMutationCodon optimized for expression in E.coliPromoterT7Available SinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLARRYv2-mScarlet
Plasmid#233210PurposeCloning expressed barcodes at the EcoRV site for preparing lentiviral libaries for single-cell lineage tracing experiments, with mScarlet markerDepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
sfGFP-pBAD
Plasmid#54519PurposeLocalization: Protein Expression Vector , Excitation: 485, Emission: 507DepositorTypeEmpty backboneTags6xHis and SfGFPExpressionBacterialAvailable SinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -