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pRM055 His-TEV-Cas9 6xNLS 0C

Plasmid

#196246

Purpose

Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)

Depositor

Ross Wilson

Article

Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

Insert

Cas9

Expression

Bacterial

Promoter

T7

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAGM22082_cRed

Plasmid

#117225

Purpose

pET-28b backbone plasmid, Level 2 Golden Gate expression plasmid.

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Sci Rep. 2019 Jul 29;9(1):10932. doi: 10

Insert

Canthaxanthin Operon

Tags

34 amino acid N-terminal linker, Hexahistidine Ta…

Expression

Bacterial

Mutation

removed Type IIS restriction sites within the bac…

Promoter

T7 RNA Polymerase

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pRCA879 - KLF5 isoform 1 Puro-T2A-GFP (pRCA845 backbone)

Plasmid

#238181

Purpose

Lentiviral vector for overexpression KLF5 ORF from a EF1a promoter

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

KLF5 (KLF5 Human, Synthetic)

Use

Lentiviral

Tags

GFP

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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FASTHDR-Cterm-mTagBFP2-Blasticidin

Plasmid

#167207

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mTagBFP2 and selection with Blasticidin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

mTagBFP2

Expression

Mammalian

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-ET-F656W

Plasmid

#72546

Purpose

expresses FLAG tagged human BRD4 ET domain (608-699 aa) with F656W mutation

Depositor

Christopher Vakoc

Article

Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

Insert

BRD4 ET (BRD4 Human)

Tags

FLAG

Expression

Mammalian

Mutation

F656W

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-ET-I654Q

Plasmid

#72545

Purpose

expresses FLAG tagged human BRD4 ET domain (608-699 aa) with I654Q mutation

Depositor

Christopher Vakoc

Article

Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

Insert

BRD4 ET (BRD4 Human)

Tags

FLAG

Expression

Mammalian

Mutation

I654Q

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-ET-L630W

Plasmid

#72543

Purpose

expresses FLAG tagged human BRD4 ET domain (608-699 aa) with L630W mutation

Depositor

Christopher Vakoc

Article

Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

Insert

BRD4 ET (BRD4 Human)

Tags

FLAG

Expression

Mammalian

Mutation

L630W

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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Cx30-msfGFP

Plasmid

#69019

Purpose

Expresses human Cx30 (GJB6 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.

Depositor

David Spray

Article

Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

Insert

Cx30 (GJB6 Human)

Tags

V206K monomerized super folder GFP

Expression

Mammalian

Promoter

c

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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TRBV20-1

Plasmid

#123454

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV20-1 (TRBV20-1 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF50-2xCSTREP

Plasmid

#136211

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF50

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF50

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pLX_TRC208-SYNJ2BP-V5

Plasmid

#92149

Purpose

Expresses V5-tagged human SYNJ2BP in mammalian cells.

Depositor

Alice Ting

Article

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

SYNJ2BP-V5 (SYNJ2BP Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

*(see below)

Promoter

CMV

Available Since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-K5-2xCSTREP

Plasmid

#136154

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K5

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

K5

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pHIV-IL13-PDGFRStalk-TM

Plasmid

#240243

Purpose

Lentiviral transfer plasmid encoding IL-13 with PDGFR stalk and TM domain

Depositor

Michael Birnbaum

Article

Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

Insert

IL-13 (IL13 Human)

Use

Lentiviral

Tags

EGFP and HA

Expression

Mammalian

Promoter

EF1a

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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MAC_C_AIFM1

Plasmid

#111659

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

AIFM1 (AIFM1 Human)

Expression

Mammalian

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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acta1-Cavin4b-Clover

Plasmid

#163854

Purpose

Zebrafish muscle specific expression of Cavin4b-Clover. Parton lab clone DQZ

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

Cavin4b (cavin4b Zebrafish)

Use

Zebrafish expression

Tags

Clover

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-CCR5

Plasmid

#98949

Purpose

Mammalian expression plasmid for N-terminal FLAG-tagged human CCR5

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CCR5 (CCR5 Human)

Tags

FLAG (dykdddd) epitope tag and Signal/leader sequ…

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pETM33_USP7_MATH

Plasmid

#178490

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

USP7_MATH (USP7 Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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acta1-Cavin1a-Clover

Plasmid

#163852

Purpose

Zebrafish muscle specific expression of Cavin1a-Clover, Parton lab clone DPR

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

Cavin1a (cavin1a Zebrafish)

Use

Zebrafish expression

Tags

Clover

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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mMib1-FLAG

Plasmid

#37116

Depositor

Martin Dorf

Article

Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

Insert

Mib1(Mus musculus mindbomb homolog 1 (Drosophila) (Mib1)) (Mib1 Mouse)

Tags

3 x FLAG

Expression

Mammalian

Mutation

ACC Kozak sequence added before ATG annotated.

Promoter

CMV

Available Since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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pEasyG2_hph

Plasmid

#184915

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_hph recombines in vivo with a PCR product from pEasyG2_mic.

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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