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NET30 pEGFP-N2 (815)

Plasmid

#62011

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

NET30 (MOSPD3 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
MTK8b_005

Plasmid

#123893

Purpose

Encodes the 5' AAV ITR cassette as a type 8b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

5' AAV ITR

Expression

Mammalian

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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MTK3b_021

Plasmid

#123802

Purpose

Encodes VPR activation domain as a Type 3b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

VPR AD

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK3a_026

Plasmid

#123778

Purpose

Encodes dsaCAS9 tagged with NLS as a Type 3a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sadCAS9 (with NLS)

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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JPF0510

Plasmid

#124001

Purpose

Encodes pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB_pEF1a-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-mNG-C-Kbp

Plasmid

#178013

Purpose

Bacterial expression of green fluorescent potassium indicator mNG-C-Kbp

Depositor

Edward Boyden

Article

Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

Insert

mNG-C-Kbp

Expression

Bacterial

Promoter

araB

Available Since

Dec. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-mNG-Pa-Kbp

Plasmid

#178014

Purpose

Bacterial expression of green fluorescent potassium indicator mNG-Pa-Kbp

Depositor

Edward Boyden

Article

Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

Insert

mNG-Pa-Kbp

Expression

Bacterial

Promoter

araB

Available Since

Dec. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-mNG-Hv-Kbp

Plasmid

#178015

Purpose

Bacterial expression of green fluorescent potassium indicator mNG-Hv-Kbp

Depositor

Edward Boyden

Article

Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

Insert

mNG-Hv-Kbp

Expression

Bacterial

Promoter

araB

Available Since

Dec. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-mNG-D-Kbp

Plasmid

#178012

Purpose

Bacterial expression of green fluorescent potassium indicator mNG-D-Kbp

Depositor

Edward Boyden

Article

Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

Insert

mNG-D-Kbp

Expression

Bacterial

Promoter

araB

Available Since

Dec. 2, 2023

Availability

Academic Institutions and Nonprofits only

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MTK678_002

Plasmid

#123923

Purpose

Encodes the TU GFP dropout cassette with Ampicillin resistance and bacterial artificial chromosome as a type 678 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

BAC-AmpR

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pPKm-118

Plasmid

#90491

Purpose

Expresses pcDNA3 - CMVmin - 5X UAS - pFR Luciferase

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

pFR Luciferase Fluc

Expression

Mammalian

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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MTK3_026

Plasmid

#123741

Purpose

Encodes cAMP reporter Flamindo2 as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Flamindo2

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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E1b-GFP-Tol2-Gateway dre SE-zic2a P

Plasmid

#89385

Purpose

Vector for reporter assay of one zebrafish super-enhancer region

Depositor

Alena Shkumatava

Article

Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

Insert

zic2a (zic2a Zebrafish)

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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pOP461

Plasmid

#85934

Purpose

Encodes the Anti-Ebola 4G7 monoclonal antibody. Chimeric - mouse variable regions, human constant regions.

Depositor

Timothy Lu

Article

Purcell et al ACS Synth Biol. 2017 Aug 17. doi: 10.102

Insert

Anti-Ebola 4G7 monoclonal antibody

Expression

Yeast

Promoter

AOX1

Available Since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pBTK550d

Plasmid

#110617

Purpose

BTK assembled broad-host-range plasmid encoding inducible GFP driven by T7 polymerase

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Expression

Bacterial

Available Since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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MTK2_008

Plasmid

#123703

Purpose

Encodes human pUbc as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

phUbC

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pBS/U6/RNAi SadBs shRNA mouse

Plasmid

#67152

Purpose

pBS/U6 containing shRNA to mouse SADBs

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

BRSK1 (Brsk1 Mouse)

Expression

Mammalian

Promoter

U6

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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hGR-Dx

Plasmid

#133408

Purpose

overexpression of a mutant human glucocorticoid receptor in mammalian cells

Depositor

Andrew Cato

Article

Heck et al EMBO J. 1994 Sep 1;13(17):4087-95.

Insert

NR3C1 (NR3C1 Human)

Expression

Mammalian

Mutation

N454D, A458T, R460D, D462C

Available Since

Dec. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pHA843 - hrp-1p::hrp-1HsLCdeltaLC

Plasmid

#139200

Purpose

Expresses hrp-1p::hrp-1HsLCdeltaLC::hrp-1 3'UTR

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Worm

Mutation

stop at beginning of last exon

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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NET55 pEGFP-N2 (610)

Plasmid

#61992

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

NET55 (APH1B Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only