We narrowed to 7,201 results for: mme

1. pTXB3 AurA

   Plasmid

   #68270

   Purpose

   bacterial expression of human AurA

   Depositor

   Stefano Ferrari

   Article

   Krystyniak et al Oncogene. 2006 Jan 19;25(3):338-48.

   Insert

   AurA (AURKA Human)
   

   Tags

   Mxe intein/chitin binding domain

   Expression

   Bacterial

   Mutation

   See depositor comment below

   Promoter

   T7

   Available Since

   Aug. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pCDNA3-Flag-hDcp2

   Plasmid

   #72213

   Purpose

   mammalian expression of Flag-tagged human Dcp2

   Depositor

   Megerditch Kiledjian

   Article

   Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

   Insert

   Dcp2 (DCP2 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Q170R (please see depositor's comment below)

   Promoter

   CMV

   Available Since

   Dec. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKD280

   Plasmid

   #125071

   Purpose

   Expression of SO_4387 under Ptac

   Depositor

   Jeffrey Tabor

   Article

   Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

   Inserts

   SO_4387 (SO_4387 )
   lacI (lacI )
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   S286L- Please see depositor comments below

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-NES-ZapCY2

   Plasmid

   #59016

   Purpose

   Cytosolic zinc-binding FRET biosensor

   Depositor

   Amy Palmer

   Article

   Qin et al ACS Chem Biol. 2013 Nov 15;8(11):2366-71

   Insert

   NES-ZapCY2 genetically encoded cytosolic Zn(II) sensor
   

   Tags

   Nuclear Exclusion Signal

   Expression

   Mammalian

   Mutation

   See comment

   Promoter

   CMV

   Available Since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

5. mCherry-E2

   Plasmid

   #175242

   Purpose

   Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   UBE2I (UBE2I Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. PXNGW

   Plasmid

   #48258

   Purpose

   SPLIT-GFP assay in plants

   Depositor

   Wolf Frommer

   Article

   Kim et al Plant Cell. 2009 Apr;21(4):1305-23. doi:

   Type

   Empty backbone

   Use

   Binary gateway vector

   Tags

   5xHIS and YFP aa1-172

   Promoter

   35S

   Available Since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

7. PXCGW

   Plasmid

   #48255

   Purpose

   Split-GFP assay in plants

   Depositor

   Wolf Frommer

   Article

   Kim et al Plant Cell. 2009 Apr;21(4):1305-23. doi:

   Type

   Empty backbone

   Use

   Binary gateway vector

   Tags

   6xHIS and CFP aa155-238

   Promoter

   35S

   Available Since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pCS2-zfKitlgb

   Plasmid

   #168200

   Purpose

   Express zebrafish Kitlgb, mRNA synthesis

   Depositor

   Petr Bartunek

   Article

   Oltova et al Blood Adv. 2020 Dec 8;4(23):5915-5924. d

   Insert

   Kitlgb (kitlgb Zebrafish)
   

   Use

   Vertebrate expression (zebrafish, chicken, xenopu…

   Expression

   Mammalian

   Mutation

   I85V, V222I, and D233N- please see depositor comm…

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA4-Claspin-Flag

   Plasmid

   #136246

   Purpose

   Protein expression of Claspin with a C terminal FLAG tag

   Depositor

   Aziz Sancar

   Article

   Sar et al J Biol Chem. 2004 Sep 17;279(38):39289-9

   Insert

   Claspin
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   E263G, Q499R, I783T, D932G and S1062G- Please see…

   Available Since

   Jan. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. CyPet-FKBP-E2

    Plasmid

    #175244

    Purpose

    Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    UBE2I (UBE2I Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. PNXGW

    Plasmid

    #48256

    Purpose

    Split-GFP assay in plants

    Depositor

    Wolf Frommer

    Article

    Kim et al Plant Cell. 2009 Apr;21(4):1305-23. doi:

    Type

    Empty backbone

    Use

    Binary gateway vector

    Tags

    6xHIS and YFP aa1-172

    Promoter

    35S

    Available Since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

12. PCXGW

    Plasmid

    #48257

    Purpose

    Split-GFP assay in plants

    Depositor

    Wolf Frommer

    Article

    Kim et al Plant Cell. 2009 Apr;21(4):1305-23. doi:

    Type

    Empty backbone

    Use

    Binary gateway vector

    Tags

    6xHIS and CFP aa155-238

    Promoter

    35S

    Available Since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

13. p667-UBC-DYRK3-V5-miniTurbo_IDG-K

    Plasmid

    #158190

    Purpose

    Gateway destination clone of DYRK3 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    DYRK3-V5-miniTurbo (DYRK3 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Mutation

    R293H- please see depositor comments

    Promoter

    Ubiquitin

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUb LwaCas13a + LwaCas13a Guide RNA

    Plasmid

    #176307

    Purpose

    Expresses LwaCas13a and its associated guide RNA in Drosophila cells

    Depositor

    Jeremy Wilusz

    Article

    Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

    Insert

    LwaCas13a
    

    Tags

    HA tag

    Expression

    Insect

    Mutation

    E656G, I1060V, D1076G- please see depositor comme…

    Promoter

    Ubi-p63e

    Available Since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNMHCII-C1C2-GFP

    Plasmid

    #46044

    Depositor

    Robert Adelstein

    Article

    Jana et al J Biol Chem. 2009 Apr 24;284(17):11563-7

    Insert

    nonmuscle myosin heavy chain II-C, isoform C1C2 (Myh14 Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV immediate early

    Available Since

    Aug. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

16. TAL2365

    Plasmid

    #36778

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    HDAC1-TALEN_Right (Hdac1 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

17. fgfprom luc

    Plasmid

    #69446

    Purpose

    basal plasmid containing minimal fgf4 promoter used for testing enhancer activity of DNAs inserted immediately upstream of the promoter.

    Depositor

    Lisa Dailey

    Article

    Murtha et al Nat Methods. 2014 May;11(5):559-65. doi:

    Insert

    minimal fgf4 promoter
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. TAL2364

    Plasmid

    #36777

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    HDAC1-TALEN_Left (Hdac1 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

19. Antares-SEPLuc

    Plasmid

    #141086

    Purpose

    precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis

    Depositor

    Jeak Ling Ding

    Article

    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

    Insert

    Antares-T2A-puromycin-EMCV IRES-SEPLuc
    

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 66 to 91 on the Luciferas…

    Promoter

    CMV

    Available Since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR-R4R3-stop

    Plasmid

    #113745

    Purpose

    Contains three stop codons in every frame to ensure a premature stop codon when introduced into the genome via homology-directed repair. Gateway multisite entry clone.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    stop cassette
    

    Use

    CRISPR; Gateway entry vector

    Mutation

    Added 3 stop codons to the original sequence; see…

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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