We narrowed to 7,105 results for: mme

1. pcDNA3.1(+)/AUG-stop-nLuc-3XFLAG

   Plasmid

   #127308

   Purpose

   Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon, but translation terminated immediately by stop codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTV-mHdac1-tdiRFP670-PEST2

   Plasmid

   #122351

   Purpose

   Targeting vector for mouse Hdac1 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   2A-tdiRFP-PEST with homology arms for Hdac1
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDUAL-P41nmt1-Nup124-GFP-MTn

   Plasmid

   #154365

   Purpose

   Yeast expression of Nup124

   Depositor

   Wanzhong He

   Article

   Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

   Insert

   Nup124 (nup124 Fission Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   See depositor comments below

   Promoter

   Available since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTV-Met-Eif4a1-tdiRFP670-PEST

   Plasmid

   #122371

   Purpose

   Targeting vector for mouse Eif4a1 to insert 2A-tdiRFP670-PEST-2A into immediately downstream of start codon.

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   2A-tdiRFP-PEST-2A with homology arms for Eif4a1
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. M26I DJ-1 TEV site pET15b

   Plasmid

   #60685

   Purpose

   Recombinant protein expression of Park7

   Depositor

   Mark Wilson

   Article

   Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

   Insert

   M26I DJ-1 (PARK7 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   M26I and TEV site was inserted (see comments belo…

   Promoter

   T7

   Available since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

6. M26L DJ-1 TEV site pET15b

   Plasmid

   #60686

   Purpose

   Recombinant protein expression of Park7

   Depositor

   Mark Wilson

   Article

   Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

   Insert

   M26L DJ-1 (PARK7 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   M26L and TEV site was inserted (see comments belo…

   Promoter

   T7

   Available since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

7. EGFP_EHMT1B1-4

   Plasmid

   #228812

   Purpose

   Expression of mouse EHMT1 with deletion of aa 809-1298

   Depositor

   Shravanti Rampalli

   Article

   Biligiri et al PLOS Biology 22(11): e3002871.

   Insert

   EHMT1 (Ehmt1 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deletion of aa 809-1298, See Depositor Comments

   Promoter

   Available since

   March 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-nucGFP11-3xPax7gRNA

   Plasmid

   #224570

   Purpose

   Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   GFP11-H2B
   

   Use

   CRISPR

   Tags

   Histone H2B (nuclear localization)

   Expression

   Mutation

   Codon optimized

   Promoter

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-3xGFP11-mem-3xPax7gRNA

   Plasmid

   #224571

   Purpose

   Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   3xGFP11
   

   Use

   CRISPR

   Tags

   Membrane Localization Signal

   Expression

   Mutation

   Codon optimized

   Promoter

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CAG-UnaG-P2A-FusionRed

    Plasmid

    #191109

    Purpose

    Mammalian cell line expression plasmids for production of UnaG and FusionRed proteins

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    UnaG
    

    Use

    AAV

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    chicken β-actin promoter

    Available since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEYFP_C1_ATRX

    Plasmid

    #221383

    Purpose

    Expresses ATRX in mammalian cells

    Depositor

    Albert Jeltsch

    Article

    Weirich et al Commun Biol. 2024 Jun 8;7(1):707. doi: 1

    Insert

    Transcriptional regulator ATRX (ATRX Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K536N (Please see depositor comments)

    Promoter

    CMV

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-VcnTS-E1015A

    Plasmid

    #213415

    Purpose

    Vinculin tension sensor (VcnTS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1015A).

    Depositor

    Brenton Hoffman

    Article

    Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

    Insert

    VcnTS-E1015A (VCL Synthetic, Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Mutated vinculin glutamic acid 1015 to alanine (E…

    Promoter

    CMV

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1-VcnTS-E1021A

    Plasmid

    #213416

    Purpose

    Vinculin tension sensor (VcnTS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1021A).

    Depositor

    Brenton Hoffman

    Article

    Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

    Insert

    VcnTS-E1021A (VCL Synthetic, Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Mutated vinculin glutamic acid 1021 to alanine (E…

    Promoter

    CMV

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-VcnCS-E1015A

    Plasmid

    #213412

    Purpose

    Vinculin conformation sensor (VcnCS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1015A).

    Depositor

    Brenton Hoffman

    Article

    Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

    Insert

    VcnCS-E1015A (VCL Synthetic, Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Mutated vinculin glutamic acid 1015 to alanine (E…

    Promoter

    CMV

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-VcnCS-E1021A

    Plasmid

    #213413

    Purpose

    Vinculin conformation sensor (VcnCS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1021A).

    Depositor

    Brenton Hoffman

    Article

    Chirasani et al Nat Commun. 2023 Dec 14;14(1):8300. doi:

    Insert

    VcnCS-E1021A (VCL Synthetic, Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Mutated vinculin glutamic acid 1021 to alanine (E…

    Promoter

    CMV

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPBpuro-UbC-canisEgf

    Plasmid

    #173845

    Purpose

    Encoding dog Egf.

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    A cDNA of canis EGF.
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R1127Q- please see dep. comments

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCherry-FKBP-E2

    Plasmid

    #175243

    Purpose

    Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin, CyPet acts as a FRET donor to Ypet

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    UBE2I (UBE2I Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLJC2-GPT1-3xFLAG

    Plasmid

    #163446

    Purpose

    lentiviral overexpression vector for human GPT1

    Depositor

    Jason Cantor

    Article

    Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

    Insert

    GPT1 (GPT Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    See: comments

    Promoter

    CMV

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTV-Ctcf-tdiRFP670-PEST

    Plasmid

    #122357

    Purpose

    Targeting vector for mouse Ctcf to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    2A-tdiRFP-PEST with homology arms for Ctcf
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTV-Dnmt3l-tdiRFP670-PEST

    Plasmid

    #122358

    Purpose

    Targeting vector for mouse Dnmt3l to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    2A-tdiRFP-PEST with homology arms for Dnmt3l
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge