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pEGB2alpha2 35s:hCas9:tNos (GB0639)

Plasmid

#68222

Purpose

Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

hCas9

Use

CRISPR and Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

35S

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pETM6-Vv4CL-CmCHS-MsCHI

Plasmid

#73402

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

Vv4CL
CmCHS
MsCHI

Expression

Bacterial

Promoter

T7

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pETM6-At4CL-PhCHS-CmCHI

Plasmid

#73393

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

At4CL
PhCHS
CmCHI

Expression

Bacterial

Promoter

T7

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA1 WT

Plasmid

#66139

Purpose

Expresses the wild type of Sulfolobus solfataricus PCNA1

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 1

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA/Bmal L95E/L150E/W427A

Plasmid

#47352

Purpose

PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Ha

Expression

Mammalian

Mutation

L95E/L150E/W427A

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pGFH14A6-12

Plasmid

#39543

Depositor

Katharina Strub

Article

Lakkaraju et al Cell. 2008 May 2;133(3):440-51.

Insert

SRP14 (SRP14 Human)

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 101-113

Promoter

CMV

Available Since

Sept. 14, 2012

Availability

Academic Institutions and Nonprofits only
pMIG AS B56 gamma3

Plasmid

#13800

Depositor

William Hahn

Article

Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

Insert

Anti-sense PP2A B56 gamma3 (PPP2R5C Human)

Use

Retroviral

Expression

Mammalian

Mutation

Anti-sense

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
sup35-R320I

Plasmid

#1199

Depositor

Susan Lindquist

Article

True et al Nature 2004 Sep 9;431(7005):184-7. Epub

Insert

Sup35p (SUP35 Budding Yeast)

Expression

Yeast

Mutation

changed arg 320 to isoleucine

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only
p2T-CMV-evoCjCas9-eAID-BlastR

Plasmid

#194062

Purpose

Mammalian expression of evoCjCas9 eAID

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-eAID

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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JW630

Plasmid

#64807

Purpose

TCP4 expression in yeast

Depositor

Detlef Weigel

Article

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

TCP4 (TCP4 Mustard Weed)

Tags

GAL4 Activation domain and HA

Available Since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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MAC_N_RS6

Plasmid

#111694

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

RS6 (RPS6 Human)

Expression

Mammalian

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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MAC_C_COX14

Plasmid

#111663

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

COX14 (C12orf62 Human)

Expression

Mammalian

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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COG4 shRNA

Plasmid

#163861

Purpose

shRNA targeting the coding sequences of COG4

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

component of oligomeric golgi complex 4 (COG4 Human)

Expression

Mammalian

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pDY156: SUZ12 px330 sgRNA Cas9 plasmid

Plasmid

#170792

Purpose

This plasmid encodes Cas9 and a sgRNA that targets the SUZ12 locus; to be used with pDY153

Depositor

Thomas Cech

Article

Youmans et al Mol Cell. 2021 Feb 4;81(3):488-501.e9. d

Insert

Cas9

Expression

Mammalian

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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LI F-G Kan

Plasmid

#104523

Purpose

LI resistance gene module

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

Kanamycin resistance gene

Use

Cloning vector

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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MAC_N_ARP3B

Plasmid

#111680

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

ARP3B (ACTR3B Human)

Expression

Mammalian

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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MAC_C_TR61B

Plasmid

#111679

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

TR61B (TRMT61B Human)

Expression

Mammalian

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pM148: pAAV.CMV-Cas13e-NT sgRNA

Plasmid

#203446

Purpose

Plasmid expressing active RfxCas13d with non-targeting gRNA

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-non targeting (NT) sgRNA
Cas13e

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Promoter

CMV and U6

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-EGFP-C-IFT74

Plasmid

#218730

Purpose

Expresses N-terminally EGFP-tagged IFT74 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Nishijima et al Mol Biol Cell. 2017 Jun 15;28(12):1652-1

Insert

IFT74 (IFT74 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-IFT43

Plasmid

#218735

Purpose

Expresses N-terminally EGFP-tagged IFT43 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Ishida et al Hum Mol Genet. 2021 Apr 26;30(3-4):213-2

Insert

IFT43 (IFT43 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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