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p.UTA.2.0 miR-27a-3p 3 perfect copies.

Plasmid

#82439

Purpose

Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-27a-3p in three copies

Depositor

Jens Gruber

Article

Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

Insert

hsa-miR-27a-3p (MIR27A Human)

Mutation

Three copies hsa-miR-27a-3p Complementary region …

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV6-XL4 ASXL1 (p.R404X) 3x FLAG

Plasmid

#74263

Purpose

Cancer-associated ASXL1 truncation mutant in mammalian expression vector

Depositor

Anjana Rao

Article

Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

Insert

ASXL1 (ASXL1 Human)

Tags

3X FLAG

Expression

Mammalian

Mutation

p.R404X truncation

Promoter

CMV

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only
pJPC12-ΔM13-mCherry-P/PM,5G6G-GFP

Plasmid

#80911

Purpose

Reporter plasmid (mCherry and GFP)

Depositor

Mark Isalan

Article

Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

Inserts

mCherry
GFP

Expression

Bacterial

Promoter

P5G6G and PM,5G6G

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223_EGFR_p.D837A

Plasmid

#82912

Purpose

Gateway Donor vector containing EGFR, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

EGFR (EGFR Human)

Use

Gateway Cloning

Mutation

D837A

Promoter

None

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMycLAM

Plasmid

#71811

Purpose

Generating transgenic flies for FLP-inducible DamID

Depositor

Bas van Steensel

Article

Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

Insert

min.hsp70P-FRT-STOP#1-FRT-Dam-Myc-LAM

Expression

Insect

Available Since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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pRVdG-mCherry-N-P-M-nucBFP-EGFP-L

Plasmid

#52484

Purpose

Expresses mCherry, nuclear mTagBFP and EGFP

Depositor

Sebastian Seung

Article

Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc

Inserts

mCherry
nuclear mTagBFP
EGFP

Use

Rabies virus

Expression

Mammalian

Promoter

CMV

Available Since

June 2, 2014

Availability

Academic Institutions and Nonprofits only

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R26/P-nlsrtTA

Plasmid

#46183

Depositor

Rudolf Jaenisch

Article

Wutz et al Nat Genet. 2002 Feb;30(2):167-74. Epub 2

Inserts

nlsrtTA
pgk-puro

Use

Mouse Targeting

Available Since

July 19, 2013

Availability

Academic Institutions and Nonprofits only
p6656 MSCV-P C-Flag HA 25E7-Kozak

Plasmid

#35149

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV25 E7

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

March 9, 2012

Availability

Academic Institutions and Nonprofits only
p-1459-adcy4-Luc

Plasmid

#28184

Depositor

Bernard Schimmer

Article

Frigeri et al Endocrinology. 2002 Oct . 143(10):4031-7

Insert

Adcy4 promoter (Adcy4 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

April 14, 2011

Availability

Academic Institutions and Nonprofits only
p-miR-Report + MT miR-181 BS in NLK 3UTR

Plasmid

#22791

Depositor

Xin Wang

Article

Ji et al Hepatology. 2009 Aug . 50(2):472-80.

Insert

Mutant miR-181 Binding Site in Nemo-like kinase 3 Untranslated Region (NLK Human)

Use

Luciferase

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only
pLINK cyclin T1 (1-280) myc (all 200) (P#575)

Plasmid

#14610

Depositor

Matija Peterlin

Article

Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

Insert

cyclin T1 (1-280) (all 200) (CCNT1 Human)

Tags

myc

Expression

Mammalian

Mutation

C198A, C200A, H202A, C205A. Active in 3T3 cells.

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pACT2 V1H (P#663)

Plasmid

#14665

Depositor

Matija Peterlin

Article

Geyer et al Mol Biol Cell. 2002 Jun . 13(6):2045-56.

Insert

V1H (ATP6V1H Human)

Tags

Gal4 AD

Expression

Yeast

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pET28_mouse-pro-IL18

Plasmid

#183389

Purpose

Overexpression of catalytic domain of inflammatory caspase in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

pro-IL18 (Il18 Mouse)

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW mNeongreen-T2A-mCherry

Plasmid

#125698

Purpose

Produces equimolar expression of mNeongreen and mCherry

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

mNeongreen-T2A-mCherry

Expression

Yeast

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pISH-Th

Plasmid

#105988

Purpose

To prepare riboprobe for in situ hybridization

Depositor

Peter Mombaerts

Article

Walz et al J Neurobiol. 2006 Jul . 66(8):835-46.

Insert

tyrosine hydroxylase (Th Mouse)

Use

To generate riboprobe

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pEB2-TagRFP

Plasmid

#103997

Purpose

Plasmid encoding TagRFP

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

TagRFP

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to eqFP578 (R32G, K42R, K67R, …

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Hygro(-)-B2-cT

Plasmid

#104355

Purpose

Expresses human beta 2 integrin tension sensor

Depositor

Pontus Nordenfelt, Timothy Springer

Article

Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10

Insert

Human integrin b2 with tension sensor (ITGB2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pEB1-mClover3

Plasmid

#103994

Purpose

Plasmid encoding mClover3

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mClover3

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S30R, Y39N, …

Promoter

proC

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pEB1-moxVenus

Plasmid

#103993

Purpose

Plasmid encoding moxVenus

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

moxVenus

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S30R, Y39N, …

Promoter

proC

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pEB1-mEmerald

Plasmid

#103982

Purpose

Plasmid encoding mEmerald

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mEmerald

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, S65T, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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