We narrowed to 10,290 results for: ADA

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pCEP4-TAPBPR-TM

Plasmid

#178645

Purpose

Mammalian expression of FLAG-tagged TAPBPR with MHC TM

Depositor

Erik Procko

Article

McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

Insert

TAPBPR (TAPBPL Human)

Tags

FLAG and Signal/leader sequence from HLA class I …

Expression

Mammalian

Mutation

switched transmembrane domain with that of MHC-I

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pD40-His/V5-c-MycS62A

Plasmid

#45599

Depositor

Rosalie Sears

Article

Yeh et al Nat Cell Biol. 2004 Apr;6(4):308-18. Epu

Insert

c-Myc S62A (Myc Mouse)

Tags

6xHis and V5

Expression

Mammalian

Mutation

S62A

Promoter

CMV

Available Since

June 10, 2013

Availability

Academic Institutions and Nonprofits only
pKK-TEV-mClover3

Plasmid

#105795

Purpose

Expression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV). mClover3 is a brighter derivative of mEGFP (PMID: 26879144).

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

TEV-mClover3

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pNOC_hlbCas12a-Nlux

Plasmid

#176240

Purpose

pNOC episomal plasmid harboring the humanized lbCas12a gene sequence tagged with Nlux under the control of Ribi promoter

Depositor

John van der Oost

Article

Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

Insert

humanized lbCas12a

Use

CRISPR, Luciferase, and Synthetic Biology ; Expre…

Tags

luciferase

Promoter

Ribosomal subunit

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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Myh6-Cas9

Plasmid

#109037

Purpose

Cardiac-specific overexpression of Cas9

Depositor

Eva van Rooij

Article

Johansen et al Circ Res. 2017 Oct 27;121(10):1168-1181.

Insert

Cas9

Tags

3XFlag

Expression

Mammalian

Promoter

alpha MHC

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-NR1H2

Plasmid

#192919

Purpose

Barcoded piggybac transposon vector with Dox-inducible expression of NR1H2

Depositor

George Church

Article

Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

Insert

NR1H2 (NR1H2 Human)

Tags

HA

Expression

Mammalian

Promoter

TET3G-miniCMV

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneoMyc UNC119Aa

Plasmid

#128331

Purpose

Mammalian expression vector of Myc-tagged human UNC119A isoform a

Depositor

Yutaka Hata

Article

Iwasa et al Cancer Sci. 2018 Sep;109(9):2767-2780. d

Insert

UNC119 (UNC119 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pGLO-GFP-3UAG

Plasmid

#82501

Purpose

Expresses GFP with 3 UAG codons at the amino acid position 3, 151, and 153

Depositor

Chang Liu

Article

Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542

Insert

Green Fluorescence Protein with 3 UAG codons

Expression

Bacterial

Mutation

Added an UAG codon at the amino acid position 3, …

Promoter

AraBAD

Available Since

Sept. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pLL5.0-Anillin shRNA

Plasmid

#187270

Purpose

Lentiviral expression of shRNA targeting Anillin

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

shRNA for Anillin (ANLN Human)

Use

Lentiviral

Tags

EGFP

Promoter

U6

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pJM185A

Plasmid

#218126

Purpose

Hybrid circuit displaying hsCRBN bait and driving expression of luxAB

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Inserts

CRBN (CRBN Human)
luxAB

Tags

434cI_RR69

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCIneoMyc UNC119Ab

Plasmid

#128332

Purpose

Mammalian expression vector of Myc-tagged human UNC119A isoform b

Depositor

Yutaka Hata

Article

Iwasa et al Cancer Sci. 2018 Sep;109(9):2767-2780. d

Insert

UNC119 (UNC119 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pNOC_hfnCas12a_NR1_NR2_LPT1

Plasmid

#176253

Purpose

pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and CRISPR array with spacers 1 and 2 targeting the Nitrate reductase gene and one spacer targeting the LPAAT gene

Depositor

John van der Oost

Article

Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

Insert

humanized fnCas12a

Use

CRISPR, Luciferase, and Synthetic Biology ; Expre…

Tags

luciferase

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-mGas8-PA

Plasmid

#176468

Purpose

Expression vector of mouse growth arrest specific 8 (Gas8) tagged with PA at C-terminus.

Depositor

Masahito Ikawa

Article

Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

Insert

growth arrest specific 8 (Gas8 Mouse)

Tags

PA

Expression

Mammalian

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1_MeCP2(R306C)

Plasmid

#110188

Purpose

Expression of mouse MeCP2 (R306C) in mammalian cells

Depositor

Adrian Bird

Article

Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

Insert

MeCP2_e2 FL [R306C] (mouse cDNA) (Mecp2 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Arg 306 Cys

Promoter

CMV

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-EGFP-CAAX

Plasmid

#208684

Purpose

Expresses the membrane-localized enhanced green fluorescent protein EGFP-CAAX in neurons

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

membrane-localized enhanced green fluorescent protein EGFP-CAAX

Use

AAV

Promoter

hSyn

Available Since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DIO-GRAB_NTS1.0

Plasmid

#208671

Purpose

Expresses the genetically-encoded fluorescent neurotensin (NTS) sensor GRAB_NTS1.0 in a cre-dependent manner

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based neurotensin (NTS) sensor GRAB_NTS1.0

Use

AAV

Promoter

EFS

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV-NSD3-short

Plasmid

#72552

Purpose

expresses 3*FLAG tagged human NSD3-short

Depositor

Christopher Vakoc

Article

Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

Insert

NSD3-short (NSD3 Human)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Mutation

none

Promoter

LTR

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Puro-sTagRFP-TUBA1B

Plasmid

#207769

Purpose

Donor template for Puro-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

TUBA1B Homology Arms flanking a Puro-sTagRFP Cassette (TUBA1B Human)

Use

CRISPR; Donor template

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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AAV-GfABC1D-SaCas9-WPRE3-pA

Plasmid

#203540

Purpose

SaCas9 expression in astrocytes

Depositor

Ben Deverman

Article

Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

Insert

SaCas9

Use

AAV

Expression

Mammalian

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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p2R3a-3xVenusYFP-OcsT

Plasmid

#71271

Purpose

Entry clone containing three repeats of Venus. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

Insert

3 times VenusYFP

Use

Gateway

Tags

octaline synthase terminator

Available Since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only

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