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Tol2-mpx:Lifeact-Ruby

Plasmid

#45246

Depositor

Anna Huttenlocher

Article

Yoo et al Dev Cell. 2010 Feb 16. 18(2):226-36.

Insert

lifeact-ruby

Use

Zebrafish expression

Promoter

mpx

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pRSET his-eGFP

Plasmid

#113551

Purpose

For bacterial expression and purification of eGFP

Depositor

Jeanne Stachowiak

Article

Busch et al Nat Commun. 2015 Jul 24;6:7875. doi: 10.

Insert

his-eGFP

Expression

Bacterial

Mutation

A206K

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pToneDest

Plasmid

#67691

Purpose

attR4-attR3 Destination cassette in Tol1 backbone

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Type

Empty backbone

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-let-7 8x

Plasmid

#20931

Depositor

Yukihide Tomari

Article

Iwasaki et al Mol Cell. 2009 Apr 10;34(1):58-67. doi:

Insert

8x let-7 target sites

Use

Luciferase

Expression

Insect and Mammalian

Available Since

June 19, 2009

Availability

Academic Institutions and Nonprofits only

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pEPkan-S

Plasmid

#41017

Purpose

PCR template for en passant (two-step Red-mediated) mutagenesis

Depositor

Nikolaus Osterrieder

Article

Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

Insert

Kana_I-SceI

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pBRIT HA/FLAG

Plasmid

#17519

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Type

Empty backbone

Use

Cre/Lox and Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pOK12

Plasmid

#49789

Purpose

E. coli cloning vector (KanR, low copy, blue/white selection)

Depositor

Joachim Messing

Article

Vieira et al Gene. 1991 Apr;100:189-94.

Type

Empty backbone

Use

Cloning vector

Promoter

lacZ

Available Since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2G2

Plasmid

#15791

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2G2 (UBE2G2 Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pMPRAdonor1

Plasmid

#49352

Purpose

Promoterless luc2 donor plasmid for MPRA library construction

Depositor

Tarjei Mikkelsen

Article

Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

Insert

luc2

Use

Luciferase

Promoter

None

Available Since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pAC-hRluc

Plasmid

#24348

Depositor

Liqun Luo

Article

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Insert

hRluc

Expression

Insect

Available Since

March 31, 2010

Availability

Academic Institutions and Nonprofits only

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MG

Plasmid

#26528

Depositor

David Baltimore

Article

O'Connell et al J Exp Med. 2008 Mar 17. 205(3):585-94.

Type

Empty backbone

Use

Retroviral

Tags

eGFP

Expression

Mammalian

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only

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lvl0 nluc CDS

Plasmid

#153404

Purpose

Nanoluciferase coding sequence

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

nanoluciferase CDS

Expression

Plant

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-HcRed

Plasmid

#11152

Purpose

Mammalian expression vector for expression of HcRed

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Insert

HcRed1 from Heteractis crispa

Expression

Mammalian

Available Since

April 13, 2006

Availability

Academic Institutions and Nonprofits only

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pAC-Luc

Plasmid

#33154

Depositor

Michael Rosbash

Article

Kadener et al Genes Dev. 2007 Jul 1;21(13):1675-86. Ep

Type

Empty backbone

Use

Luciferase

Tags

Luciferase

Expression

Insect

Promoter

Actin 5C

Available Since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
GST-PRMT1

Plasmid

#34699

Depositor

Steven Clarke

Article

Gary et al J Biol Chem. 1996 May 24;271(21):12585-9

Insert

PRMT1 (Prmt1 Rat)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
Copia Renilla Control

Plasmid

#38093

Depositor

Philip Beachy

Article

Lum et al Science. 2003 Mar 28;299(5615):2039-45.

Insert

renilla luciferase

Use

Luciferase

Expression

Insect

Promoter

copia

Available Since

Nov. 12, 2012

Availability

Academic Institutions and Nonprofits only
pARE-lux

Plasmid

#11768

Depositor

Joan Massague, Jeff Wrana

Article

Wrana et al Cell. 1992 Dec 11. 71(6):1003-14.

Insert

ARE

Use

Luciferase

Expression

Mammalian

Available Since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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psi-CHECK3

Plasmid

#136010

Purpose

psi-CHECK2 plasmid modified so both Renilla and Firefly luciferase contain introns

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Type

Empty backbone

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2E1

Plasmid

#15787

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2E1 (UBE2E1 Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pJFRC161-20XUAS-IVS-KD::PEST

Plasmid

#32140

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

KD::PEST

Expression

Insect

Available Since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

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