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pCMV-Tag2B-NCreERT2

Plasmid

#106370

Purpose

N-terminal part of the split-CreERT2 System

Depositor

Johannes Hirrlinger

Article

Hirrlinger et al PLoS One. 2009 Dec 16;4(12):e8354. doi:

Insert

NCreERT2

Use

Cre/Lox

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag3B-ERT2CCre

Plasmid

#106371

Purpose

C-terminal part of the split-CreERT2 System

Depositor

Johannes Hirrlinger

Article

Hirrlinger et al PLoS One. 2009 Dec 16;4(12):e8354. doi:

Insert

ERT2CCre

Use

Cre/Lox

Tags

c-myc

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pVP073

Plasmid

#222076

Purpose

eforRED visually marked spectinomycin-resistant pVS1 plasmid backbone containing the sacB eviction marker and an excisable ccdb/CmR cassette flanked by EcoRV-HindIII restriction sites.

Depositor

Wayne Parrott

Article

Pennetti et al Biodes Res. 2025 Feb 27;7(1):100001. doi

Type

Empty backbone

Use

Chromoprotein-marked pvs1 backbone

Available Since

Jan. 27, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pVP074

Plasmid

#222077

Purpose

eforRED visually marked kanamycin-resistant pVS1 plasmid backbone containing the sacB eviction marker and an excisable ccdb/CmR cassette flanked by EcoRV-HindIII restriction sites.

Depositor

Wayne Parrott

Article

Pennetti et al Biodes Res. 2025 Feb 27;7(1):100001. doi

Type

Empty backbone

Use

Chromoprotein-marked pvs1 backbone

Available Since

Jan. 27, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pVP075

Plasmid

#222078

Purpose

AeBlue visually marked kanamycin-resistant pVS1 plasmid backbone containing the sacB eviction marker and an excisable ccdb/CmR cassette flanked by EcoRV-HindIII restriction sites.

Depositor

Wayne Parrott

Article

Pennetti et al Biodes Res. 2025 Feb 27;7(1):100001. doi

Type

Empty backbone

Use

Chromoprotein-marked pvs1 backbone

Available Since

Jan. 27, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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DHFR-TEV (integrating)

Plasmid

#247218

Purpose

Plasmid for genomic integration of DHFR-TEV into the YORWΔ22 locus of yeast cells.

Depositor

Johannes Herrmann

Article

Hoffman et al FEBS Lett. 2025 Oct 25. doi: 10.1002/187

Insert

DHFR-TEV

Expression

Yeast

Promoter

TEF2

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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Atp5-DHFR-TEV (integrating)

Plasmid

#247219

Purpose

Plasmid for genomic integration of Atp5 (MTS)-DHFR-TEV into the YORWΔ22 locus of yeast cells.

Depositor

Johannes Herrmann

Article

Hoffman et al FEBS Lett. 2025 Oct 25. doi: 10.1002/187

Insert

Atp5 MTS-DHFR-TEV

Expression

Yeast

Promoter

TEF2

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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Oxa1-DHFR-TEV (integrating)

Plasmid

#247220

Purpose

Plasmid for genomic integration of Oxa1(MTS)-DHFR-TEV into the YORWΔ22 locus of yeast cells.

Depositor

Johannes Herrmann

Article

Hoffman et al FEBS Lett. 2025 Oct 25. doi: 10.1002/187

Insert

Oxa1 MTS-DHFR-TEV

Expression

Yeast

Promoter

TEF2

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-eYFP

Plasmid

#104056

Purpose

An AAV genome with tet-inducible expression of the fluorescent protein eYFP

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

EYFP

Use

AAV

Promoter

TRE

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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Psy074

Plasmid

#99504

Purpose

Bacterial expression of LIPS M1+TE

Depositor

Jay Keasling

Article

Yuzawa et al ACS Synth Biol. 2017 Jan 20;6(1):139-147

Insert

LIPS M1+TE

Expression

Bacterial

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-mTurquoise2

Plasmid

#104059

Purpose

AAV expression of Cre-dependent reporter mTurquoise2

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

mTurquoise2

Use

AAV

Promoter

CAG

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pNMT

Plasmid

#42578

Depositor

James Hurley

Article

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

Insert

N-myristoyl tranferase (NMT1 Budding Yeast)

Expression

Bacterial

Mutation

two silent mutations T690C and T942C

Promoter

T7

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only
pET15b PKA Cat

Plasmid

#14921

Depositor

Susan Taylor

Article

Narayana et al Biochemistry. 1997 Apr 15. 36(15):4438-4

Insert

PKA catalytic subunit alpha (Prkaca Mouse)

Tags

6xHis and Thrombin cleavage site

Expression

Bacterial

Available Since

July 24, 2007

Availability

Academic Institutions and Nonprofits only

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pDEST-ORF-V2

Plasmid

#73638

Purpose

Gateway™ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 2 (V2).

Depositor

Darren Saunders

Article

Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

Type

Empty backbone

Tags

Venus fragment 2 (V2)

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCW-EGFP-Blast

Plasmid

#246561

Purpose

Lentiviral vector for inducible expression of EGFP (all-in-one Tet-On system)

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

GFP

Use

Lentiviral

Mutation

F64L, S65T (EGFP)

Promoter

tight TRE

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Fzd1/2/4/5/7/8 gRNA

Plasmid

#246550

Purpose

CRISPR vector co-expressing Cas9 and a mouse Fzd1/2/4/5/7/8 gRNA (conserved region)

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Fzd1/2/4/5/7/8 gRNA

Use

CRISPR

Promoter

U6

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Dvl1/3 gRNA

Plasmid

#246555

Purpose

CRISPR vector co-expressing Cas9 and a mouse Dvl1/3 gRNA (conserved region)

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Dvl1/3 gRNA

Use

CRISPR

Promoter

U6

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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TLR4

Plasmid

#20863

Depositor

Jie-Oh Lee

Article

Park et al Nature. 2009 Apr 30;458(7242):1191-5. do

Insert

Toll-Like Receptor 4 (TLR4 Human)

Expression

Insect

Mutation

Contains ectodomain (amino acids 27–631)

Available Since

May 20, 2009

Availability

Academic Institutions and Nonprofits only

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pNG1299

Plasmid

#149709

Purpose

Template for Malachite Green Aptamer (MGA) transcription assay

Depositor

Peter Lewis

Article

Newing et al Nat Commun. 2020 Dec 18;11(1):6420. doi:

Insert

Triple Promoter - 12X MG Aptamer Array - Terminator construct

Use

Synthetic Biology

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST-ORF-V1

Plasmid

#73637

Purpose

Gateway™ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 1 (V1)

Depositor

Darren Saunders

Article

Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

Type

Empty backbone

Tags

Venus fragment 1 (V1)

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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