We narrowed to 9,414 results for: pho;

1. MPC026: pAAV-CAG-FLEX-NovArch-TS-Citrine_TSx3-ER2

   Plasmid

   #153195

   Purpose

   encodes pAAV NovArch

   Depositor

   Adam Cohen

   Article

   Chien et al Sci Adv. 2021 May 5;7(19). pii: 7/19/eab

   Insert

   NovArch
   

   Use

   AAV

   Tags

   citrine

   Expression

   Mammalian

   Available Since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS405 VAC8-G2A,C4A,C5A,C7A-RFP

   Plasmid

   #166842

   Purpose

   Expresses a myristoylation and palmitoylation mutant Vac8-RFP in yeasts, mutations are Gly2Ala, Cys4Ala, Cys5Ala, Cys7Ala

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

   Insert

   VAC8 (VAC8 Budding Yeast)
   

   Tags

   RFP

   Expression

   Yeast

   Mutation

   G2A, C4A, C5A, C7A

   Promoter

   VAC8 promoter

   Available Since

   April 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS405 VAC8-G2A,C4A,C5A,C7A-GFP

   Plasmid

   #166839

   Purpose

   Expresses a myristoylation and palmitoylation mutant Vac8-GFP in yeasts, mutations are Gly2Ala, Cys4Ala, Cys5Ala, Cys7Ala

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

   Insert

   VAC8 (VAC8 Budding Yeast)
   

   Tags

   GFP

   Expression

   Yeast

   Mutation

   G2A, C4A, C5A, C7A

   Promoter

   VAC8 promoter

   Available Since

   April 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS405 VAC8-C4A,C5A,C7A-GFP

   Plasmid

   #166838

   Purpose

   Expresses a palmitoylation mutant Vac8-GFP in yeasts, mutations are Cys4Ala, Cys5Ala, Cys7Ala

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

   Insert

   VAC8 (VAC8 Budding Yeast)
   

   Tags

   GFP

   Expression

   Yeast

   Mutation

   C4A, C5A, C7A

   Promoter

   VAC8 promoter

   Available Since

   April 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-GFAP104-ChromeT-mCherry

   Plasmid

   #123126

   Purpose

   AAV-mediated expression of ChromeT-mCherry under the GFAP promoter.

   Depositor

   Edward Boyden

   Article

   Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

   Insert

   ChromeT-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Channelrhodopsin-2 (ChR2) with mutations A71S/ E9…

   Promoter

   GFAP104

   Available Since

   April 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. CMV-mEGFP(A206K)-paCaMKII(I539E)-ShadowG

   Plasmid

   #167478

   Purpose

   Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

   Depositor

   Hideji Murakoshi

   Article

   Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

   Insert

   mEGFP(A206K)-paCaMKII(I539E)-ShadowG
   

   Tags

   ShadowG and mEGFP(A206K)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMV-mEGFP(A206K)-paCaMKII(C450A)-ShadowG

   Plasmid

   #167479

   Purpose

   Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

   Depositor

   Hideji Murakoshi

   Article

   Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

   Insert

   mEGFP(A206K)-paCaMKII(C450A)-ShadowG
   

   Tags

   ShadowG and mEGFP(A206K)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBz172_CsoS2_N1_GFP

   Plasmid

   #140852

   Purpose

   His-tagged first N-peptide of NTD fused to Superfolder GFP ([N1]-GFP) used for BLI and MST, pET-14b backbone

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CsoS2 NTD repeat 1 fused to GFP
   

   Tags

   Hexahistidine tag, Strep-Tag II, and Superfolder …

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBz175_CsoS2_N4_GFP

   Plasmid

   #140855

   Purpose

   His-tagged fourth N-peptide of NTD fused to Superfolder GFP ([N4]-GFP) used for BLI, pET-14b backbone

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CsoS2 NTD repeat 4 fused to GFP
   

   Tags

   Hexahistidine tag, Strep-Tag II, and Superfolder …

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBz173_CsoS2_N2_GFP

    Plasmid

    #140853

    Purpose

    His-tagged second N-peptide of NTD fused to Superfolder GFP ([N2]-GFP) used for BLI, pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CsoS2 NTD repeat 2 fused to GFP
    

    Tags

    Hexahistidine tag, Strep-Tag II, and Superfolder …

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBz174_CsoS2_N3_GFP

    Plasmid

    #140854

    Purpose

    His-tagged third N-peptide of NTD fused to Superfolder GFP ([N3]-GFP) used for BLI, pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CsoS2 NTD repeat 3 fused to GFP
    

    Tags

    Hexahistidine tag, Strep-Tag II, and Superfolder …

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLz26_CsoS2_N1-N2_GFP

    Plasmid

    #140851

    Purpose

    His-tagged first two N-peptides of NTD fused to Superfolder GFP ([N1-N2]-GFP) used for MST, pET-14b backbone

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    CsoS2 NTD repeats 1-2 fused to GFP
    

    Tags

    Hexahistidine tag, Strep-Tag II, and Superfolder …

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTAMACHis6_PiuD

    Plasmid

    #128953

    Purpose

    expresses PiuD in E.coli

    Depositor

    James Naismith

    Article

    Luscher et al Antimicrob Agents Chemother. 2018 May 25

    Insert

    Putative outer membrane receptor for iron transport (PiuD)
    

    Tags

    6xHis and TamA

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed (1-105)

    Promoter

    T7

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMH-HT_SsCHAD-H29A-R32A-R36A

    Plasmid

    #127786

    Purpose

    Expresses a SsCHAD mutant protein in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    SsCHAD (SULM_RS11045 Sulfolobus solfataricus)
    

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    mutations in His29, Arg32 and Arg36 to Ala

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMH-HT_CtCHAD-R438A-K441A

    Plasmid

    #127791

    Purpose

    Expresses a CtCHAD mutant protein in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    CtCHAD (CT0884 Chlorobium tepidum)
    

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    Residues 208-522 (CHAD domain), mutations in Arg4…

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMH-HT_ygiF-TTM

    Plasmid

    #127794

    Purpose

    Expresses the TTM/CYTH domain of ygiF in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    ygiF (ygiF Escherichia coli)
    

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    N-terminal TTM domain of ygiF (residues 1-200)

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX-4T-3-cytXlATL2-R232Q

    Plasmid

    #124064

    Purpose

    Control protein. The R232Q mutation abolishes dimerization activity, so this construct is inactive compared to wild type cytATL

    Depositor

    Tom Rapoport

    Article

    Wang et al J Cell Biol. 2013 Dec 9;203(5):801-14. d

    Insert

    cytXlATL2-R232Q
    

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJet-CMV-CD5cmyc2-bglpA

    Plasmid

    #110645

    Purpose

    Expression plasmid for c-Myc-tag embedded into human CD52 with short polyA

    Depositor

    Dmitriy Mazurov

    Article

    Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

    Insert

    CMV-CDcmyc2-bglpA
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-FLEX-mCherry-FHA1-subT391A-mCherry

    Plasmid

    #104462

    Purpose

    Cre-dependent, Non-phosphorylatable PKA consensus substrate tagged with mCherry

    Depositor

    Bernardo Sabatini

    Article

    Chen et al Neuron. 2017 Dec 6;96(5):1070-1083.e5. d

    Insert

    mCherry-subT391A-FHA1-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Promoter

    CAG

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSC27

    Plasmid

    #104801

    Purpose

    CRISPR/Cas9 2xplex gRNA targeting Medtr4g020620 (Pho2ab). Also expresses Cas9 from Gmubi promoter.

    Depositor

    Nevin Young

    Article

    Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

    Insert

    Medtr4g020620
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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