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pEMG-RIS

Plasmid

#225132

Purpose

For genome editing in P. putida KT2440, rhamnose is used for the induction of I-sceI gene. Km.

Depositor

Lars Blank

Article

Meng et al Microb Biotechnol. 2024 Jul;17(7):e14531

Type

Empty backbone

Expression

Bacterial

Available Since

Oct. 10, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHR_D17_hrGFP

Plasmid

#84616

Purpose

HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into D17 locus

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

hrGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

UAS1B8-TEF(136)

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pHR_MFE1_hrGFP

Plasmid

#84617

Purpose

HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into MFE1 locus

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

hrGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

UAS1B8-TEF(136)

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pBlueSKMimRNA

Plasmid

#102535

Purpose

Template for synthesis of Minos transposase mRNA

Depositor

Michalis Averof

Article

Pavlopoulos et al Genetics. 2004 Jun;167(2):737-46.

Insert

Minos transposase mRNA

Available Since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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pDASH-DIS-α2

Plasmid

#242520

Purpose

Donor I vector in DASH and alpha2 vector in GoldenBraid/DASH, with SacB counter-selection, with attBTT and attBCC-FRT

Depositor

Jose Alonso, Anna Stepanova

Article

Zhao et al Plant Biotechnol J. 2025 Jun 10. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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12XCSL-DsRedExpressDL

Plasmid

#47683

Purpose

Notch reporter

Depositor

Urban Lendahl

Article

Hansson et al Dev Neurosci. 2006;28(1-2):118-27.

Insert

TP-1 promoter

Use

Notch reporter construct

Tags

dsRedExpressDR

Mutation

hexamerized 50-bp EBNA2 response element of the T…

Promoter

NA

Available Since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pMyBADNT

Plasmid

#84690

Purpose

Protein expression in mycobacteria

Depositor

Matthias Wilmanns

Article

Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

Type

Empty backbone

Tags

His6

Expression

Bacterial

Promoter

AraC

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-puro

Plasmid

#45561

Depositor

Michael McVoy

Article

Abbate et al Biotechniques. 2001 Aug;31(2):336-40.

Insert

Puromycin N-acetyl transferase

Use

Bifunctional selection protein

Tags

EGFP

Expression

Mammalian

Promoter

Phcmv (Strong human cytomegalovirus immediate ear…

Available Since

June 13, 2013

Availability

Academic Institutions and Nonprofits only

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pet26-MBP-ELIC

Plasmid

#39239

Depositor

Raimund Dutzler

Article

Hilf et al Nature. 2009 Jan 1. 457(7225):115-8.

Insert

Eriwinia chrysanthemi - pentameric ligand gated ion channel

Tags

His-10 and MBP

Expression

Bacterial

Promoter

T7

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pGYL1_pPromALB_FireflyLuc

Plasmid

#220282

Purpose

Firefly luciferase with minimal Albumin promoter without enhancer; enhancer of interest can be cloned into MCS

Depositor

David Waxman

Article

Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

Type

Empty backbone

Use

Luciferase

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-rtTA

Plasmid

#163601

Purpose

Expresses rtTA in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

rtTA

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pTF101.1

Plasmid

#134770

Purpose

A binary plasmid containing double CaMV 35S promoter and TEV enhancer to drive bialophos resistance gene (bar) for plant transformation.

Depositor

Kan Wang

Article

et al Euphytica (2004) 136: 167.

Type

Empty backbone

Expression

Plant

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV-CMV-DsRed-IRES-d1EGFP

Plasmid

#41942

Purpose

Retroviral expression of DsRed in mammalian cells

Depositor

Stephen Elledge

Article

Yen et al Science. 2008 Nov 7;322(5903):918-23. do

Insert

DsRed

Use

Retroviral

Expression

Mammalian

Promoter

TRE

Available Since

May 2, 2013

Availability

Academic Institutions and Nonprofits only

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pYD1-mSA

Plasmid

#39865

Depositor

Sheldon Park

Article

Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

Insert

monomeric streptavidin

Tags

Aga2, FLAG, and HA

Expression

Yeast

Available Since

March 15, 2013

Availability

Academic Institutions and Nonprofits only

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pRSET-mSA

Plasmid

#39860

Depositor

Sheldon Park

Article

Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

Insert

monomeric streptavidin

Tags

6xHis and FLAG

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2013

Availability

Academic Institutions and Nonprofits only

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pHR_AXP_hrGFP

Plasmid

#84613

Purpose

HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into AXP locus

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

hrGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

UAS1B8-TEF(136)

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pHR_XPR2_hrGFP

Plasmid

#84614

Purpose

HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into XPR2 locus

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

hrGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

UAS1B8-TEF(136)

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pHR_A08_hrGFP

Plasmid

#84615

Purpose

HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into A08 locus

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

hrGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

UAS1B8-TEF(136)

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

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TRPM4

Plasmid

#25903

Depositor

Craig Montell

Article

Xu et al Proc Natl Acad Sci U S A. 2001 Sep 11. 9

Insert

TRPM4 (TRPM4 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pEMG-AIS

Plasmid

#225133

Purpose

For genome editing in P. putida KT2440, arabinose is used for the induction of I-sceI gene. Km.

Depositor

Lars Blank

Article

Meng et al Microb Biotechnol. 2024 Jul;17(7):e14531

Type

Empty backbone

Expression

Bacterial

Available Since

Oct. 10, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge