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pHIV-IL13-TetCoil

Plasmid

#240244

Purpose

Lentiviral transfer plasmid encoding IL13 on tetrameric coiled coil

Depositor

Michael Birnbaum

Article

Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

Insert

IL-13 (IL13 Human)

Use

Lentiviral

Tags

EGFP and HA

Expression

Mammalian

Promoter

EF1a

Available Since

July 18, 2025

Availability

Academic Institutions and Nonprofits only

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TRAV41

Plasmid

#237995

Purpose

Encodes TRAV41 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBC-P2A-TRAV41 (TRAV41 Human)

Use

Synthetic Biology

Mutation

None

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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TRAV27

Plasmid

#237985

Purpose

Encodes TRAV27 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBC-P2A-TRAV27 (TRAV27 Human)

Use

Synthetic Biology

Mutation

None

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV7-3

Plasmid

#237922

Purpose

Encodes TRBV7-3 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBV7-3-TRAC (TRBV7-3 Human)

Use

Synthetic Biology

Mutation

None

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV6-2

Plasmid

#237914

Purpose

Encodes TRBV6-2 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBV6-2-TRAC (TRBV6-2 Human)

Use

Synthetic Biology

Mutation

None

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV6-1

Plasmid

#237913

Purpose

Encodes TRBV6-1 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBV6-1-TRAC (TRBV6-1 Human)

Use

Synthetic Biology

Mutation

None

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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TRAV21

Plasmid

#237978

Purpose

Encodes TRAV21 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBC-P2A-TRAV21 (TRAV21 Human)

Use

Synthetic Biology

Mutation

None

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV10-2

Plasmid

#237930

Purpose

Encodes TRBV10-2 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBV10-2-TRAC (TRBV10-2 Human)

Use

Synthetic Biology

Mutation

None

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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TRAV6

Plasmid

#237957

Purpose

Encodes TRAV6 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBC-P2A-TRAV6 (TRAV6 Human)

Use

Synthetic Biology

Mutation

None

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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TRAV7

Plasmid

#237958

Purpose

Encodes TRAV7 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBC-P2A-TRAV7 (TRAV7 Human)

Use

Synthetic Biology

Mutation

None

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV6-9

Plasmid

#237920

Purpose

Encodes TRBV6-9 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBV6-9-TRAC (TRBV6-9 Human)

Use

Synthetic Biology

Mutation

None

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV6-5

Plasmid

#237917

Purpose

Encodes TRBV6-5 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBV6-5-TRAC (TRBV6-5 Human)

Use

Synthetic Biology

Mutation

None

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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TRAV3

Plasmid

#237954

Purpose

Encodes TRAV3 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBC-P2A-TRAV3 (TRAV3 Human)

Use

Synthetic Biology

Mutation

None

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV4-1

Plasmid

#237905

Purpose

Encodes TRBV4-1 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBV4-1-TRAC (TRBV4-1 Human)

Use

Synthetic Biology

Mutation

None

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV3-1

Plasmid

#237904

Purpose

Encodes TRBV3-1 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBV3-1-TRAC (TRBV3-1 Human)

Use

Synthetic Biology

Mutation

None

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pMT-sfGFP-TurboID-ER

Plasmid

#240230

Purpose

Expression of ER-localized sfGFP-TurboID under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Insert

BIP-sfGFP-TurboID-KDEL

Expression

Insect

Promoter

MT

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pMT-sfGFP-TurboID

Plasmid

#240231

Purpose

Expression of sfGFP-TurboID under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Insert

sfGFP-TurboID

Expression

Insect

Promoter

MT

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pMT-Myc-BirA*G3-ER

Plasmid

#240234

Purpose

Expression of ER-localized Myc-BirA*-G3 under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Insert

BiP-Myc-BirA*G3-KDEL

Expression

Insect

Promoter

MT

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pWal10-roe-sfGFP

Plasmid

#240239

Purpose

Gateway destination plasmid to generate C-terminal sfGFP fusions under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Type

Empty backbone

Use

Gateway destination vector

Expression

Insect

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR_spatzle-HA

Plasmid

#240227

Purpose

Gateway entry clone with spatzle tagged with HA (contains stop codon)

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv 2025.07.09.659702; doi: https://

Insert

spatzle (spz Fly)

Use

Gateway shuttle vector

Tags

HA-spGFP11

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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