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pDEST53-LRRK2-3XKD-Y1699C

Plasmid

#25051

Depositor

Mark Cookson

Article

Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

Insert

LRRK2 (LRRK2 Human)

Tags

GFP

Expression

Mammalian

Mutation

"Kinase Dead" Lysine 1906 mutated to A…

Available Since

June 29, 2010

Availability

Academic Institutions and Nonprofits only
pRShygro shID3#1

Plasmid

#19164

Depositor

Joan Massague

Article

Gupta et al Proc Natl Acad Sci U S A. 2007 Dec 4. 10

Insert

small hairpin RNA against inhibitor of DNA binding 3 (ID3 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Sept. 16, 2008

Availability

Academic Institutions and Nonprofits only
pCMV-HA-MyD88-P200H

Plasmid

#12288

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

MyD88 (MYD88 Human)

Tags

HA

Expression

Mammalian

Mutation

P200H

Available Since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
pCMV-HA-MyD88-I179N

Plasmid

#12295

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

MyD88 (MYD88 Human)

Tags

HA

Expression

Mammalian

Mutation

I179N

Available Since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
GST-SmB

Plasmid

#1841

Depositor

Philip Leder

Article

Bedford et al Proc Natl Acad Sci U S A 1998 Sep 1;95(1

Insert

SmB (SNRPB Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only
Cav2.1 HA pcDNA3

Plasmid

#206069

Purpose

expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

Insert

cacna1a (Cacna1a Rat)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 GIL-11 myc TEV TwinStrep

Plasmid

#199627

Purpose

Eucaryotic expression plasmid for chimeric cytokine of IL-11 and LIF (GIL-11) with a TwinStrep-tag

Depositor

Jurgen Scheller

Article

Rafii et al Commun Biol. 2023 Apr 15;6(1):418. doi:

Insert

GIL-11

Tags

TwinStrep-tag

Expression

Mammalian

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pET28-MHL: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2

Plasmid

#167849

Purpose

Bacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNA

Depositor

Cheryl Arrowsmith

Article

Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

Insert

nsp10, nsp16

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 22, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pGL3-sgACSL3.C-Cas9-P2A-Puro

Plasmid

#129412

Purpose

Encoding Cas9 and sgACSL3.C for CRISPR/Cas9 mediated HDR tagging of endogenous human ACSL3 C-terminus

Depositor

Elina Ikonen

Article

Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

Insert

SpCas9 and sgACSL3.C (ACSL3 Human)

Use

CRISPR

Tags

P2A-Puro

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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VN-PTEN

Plasmid

#89331

Purpose

expresses hPTEN fused to the N-terminal part of Venus

Depositor

Tiago Outeiro

Article

Marciniak et al J Exp Med. 2017 Aug 7;214(8):2257-2269.

Insert

PTEN1 (PTEN Human)

Tags

N-terminal part of Venus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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MIG-eIF4A1 Q195E

Plasmid

#70044

Purpose

Expression of eIF4A1 Q195E mutant in mammalian cells

Depositor

Hans-Guido Wendel

Article

Wolfe et al Nature. 2014 Sep 4;513(7516):65-70. doi:

Insert

eIF4A1 (Eif4a1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

Q195E

Promoter

CMV

Available Since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only
TRCN0000231737

Plasmid

#78162

Purpose

Luciferase control

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

Luciferase

Use

RNAi

Promoter

hU6 and hU6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
TRCN0000297275

Plasmid

#78152

Purpose

shCDK4 targeting 3UTR

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

CDK4 (CDK4 Human)

Use

RNAi

Promoter

hU6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
MIG-eIF4A1 F163L

Plasmid

#70043

Purpose

Expression of eIF4A1 F163L mutant in mammalian cells

Depositor

Hans-Guido Wendel

Article

Wolfe et al Nature. 2014 Sep 4;513(7516):65-70. doi:

Insert

eIF4A1 (Eif4a1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

F163L

Promoter

CMV

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-ARAP3(C504A)

Plasmid

#39485

Depositor

Sonja Vermeren

Article

Krugmann et al Mol Cell. 2002 Jan;9(1):95-108.

Insert

ARAP3(C504A) (ARAP3 Human)

Tags

EGFP

Expression

Mammalian

Mutation

C504A = Arf GAP domain mutation

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pSSRB069_pAC8-hsDDB1dB

Plasmid

#218790

Purpose

Insect cell expression vector for 6xHis-TEV-hsDDB1∆B

Depositor

Eric Fischer

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Insert

DNA damage-binding protein 1 (DDB1 Human)

Tags

6xHis-TEV

Expression

Insect

Mutation

Deleted amino acids 396-705 and replaced with GNG…

Promoter

polyhedrin

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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PB-Ef1a-PP7 coat protein-Halo-GB1x3-WPRE

Plasmid

#198337

Purpose

PB plasmid expressing Halo-tagged PP7 coat protein

Depositor

Michael Ward

Article

De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

Insert

PP7 coat protein 3x Halo-GB1

Use

Piggybac

Tags

HaloTag

Expression

Mammalian

Promoter

Ef1a

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO5.U6.DR130(CasRX)_Non-targeting sgRNA.EFS.tRFP657

Plasmid

#212962

Purpose

Lentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.

Depositor

Roland Rad

Article

Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

Insert

DR1_CasRx_non-targeting spacer

Use

CRISPR and Lentiviral

Tags

TagRFP657

Expression

Mammalian

Promoter

U6

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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SpCas9-mSA*

Plasmid

#182952

Purpose

Express SpCas9-mSA*

Depositor

Wen Xue

Article

Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

Insert

SpCas9-mSA*

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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PP023 - CAG::QuasAr6a-TS-dmCitrine-TSx3-ER2-p2a- jRGECO1a-CAAX

Plasmid

#182063

Purpose

Co-expression of Arch-based voltage indicator (QuasAr6a) and membrane-localized calcium reporter (jRGECO1a-CAAX)

Depositor

Adam Cohen

Article

Landau et al eLife. 2022 Mar 23;11. pii: 76993. doi:

Insert

QuasAr6a-TS-dmCitrine-TSx3-ER2-p2a- jRGECO1a-CAAX

Use

Lentiviral

Promoter

CAG

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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