165,971 results

1. pKB01_mCherry

   Plasmid

   #69104

   Purpose

   Expresses mCherry (codon adapted for Bacillus subtilis); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

   Depositor

   Jan-Willem Veening

   Article

   Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

   Insert

   mCherry
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Oct. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAS9c

   Plasmid

   #141250

   Purpose

   Constitutive expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

   Depositor

   Adam Bertl

   Article

   Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   TEF1 (Cas9), pSNR52 (gRNA)

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. MSCV-IRES-NUP98::NSD1-GFP

   Plasmid

   #236201

   Purpose

   Expresses NUP98::NSD1 fusion protein in mammalian cells

   Depositor

   Michael Heuser

   Article

   Mohanty et al Blood Adv. 2025 May 7:bloodadvances.2024

   Insert

   NUP98::NSD1 (NUP98, NSD1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   MSCV

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-T7-SpRY-P2A-EGFP (RTW4830)

   Plasmid

   #139989

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. VAChT-pHuji

   Plasmid

   #105319

   Purpose

   VAChT tagged with pH-sensitive red fluorescent protein pHuji

   Depositor

   Justin Taraska

   Article

   Martineau et al Nat Commun. 2017 Nov 10;8(1):1412. doi:

   Insert

   VAChT (Slc18a3 Rat)
   

   Tags

   pHuji

   Expression

   Mammalian

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Mitochondrial cpGFP

   Plasmid

   #215711

   Purpose

   Expresses cpGFP in the mitochondria

   Depositor

   Katharine Diehl

   Article

   Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

   Insert

   cpGFP
   

   Use

   Lentiviral

   Tags

   FLAG, HA, and mitochondrial localization sequence…

   Expression

   Mammalian

   Mutation

   circularly permuted GFP

   Promoter

   CMV

   Available Since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAS9id

   Plasmid

   #141251

   Purpose

   Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

   Depositor

   Adam Bertl

   Article

   Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   GAL1 (Cas9), pSNR52 (gRNA)

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SuperClomeleon

   Plasmid

   #203724

   Purpose

   An improved engineered fluorescent ratiometric optogenetic sensor to detect inhibitory synaptic activity via changes in intracellular chloride

   Depositor

   George Augustine

   Article

   Grimley et al J Neurosci. 2013 Oct 9;33(41):16297-309.

   Insert

   Cerulean linked topaz fluorescent protein
   

   Expression

   Bacterial and Mammalian

   Mutation

   Cerulean, modified Topaz Fluorescent Protein with…

   Available Since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEGFP-N1-PARP1

   Plasmid

   #211578

   Purpose

   CMV driven PARP1-eGFP expressing construct with IRES-Neomycin selection

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

   Insert

   PARP1 (PARP1 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHAGE-EF1aL-WT_ABCA3-GFP

    Plasmid

    #188548

    Purpose

    Lentiviral vector allowing for EF1aL-driven constitutive expression of wildtype human ABCA3:GFP fusion protein

    Depositor

    Darrell Kotton

    Article

    Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

    Insert

    ABCA3:GFP (ABCA3 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Promoter

    EF1aL

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge