We narrowed to 15,723 results for: LEA

1. pAAV CMV-DIO-(mCherry-U6)-shRNA(scrambled)

   Plasmid

   #214733

   Purpose

   Expresses mCherry protein in infected and cre positive cells

   Depositor

   Félix Leroy

   Article

   de Leon Reyes et al Cell. 2023 Sep 14;186(19):4152-4171.e31.

   Insert

   anti-Crh shRNA (scrambled) / mCherry
   

   Use

   AAV

   Available Since

   May 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVH-EF1a-GFP-P2A-SOX2

   Plasmid

   #130693

   Purpose

   Lentiviral plasmids encoding human SOX2 with co-expressoin of GFP mediated by P2A.

   Depositor

   Kejin Hu

   Article

   Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

   Insert

   human SOX2 (SOX2 Human)
   

   Use

   Lentiviral

   Available Since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV_eA3A-NG-nCas9

   Plasmid

   #163536

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   eA3A-NG-nCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

   Available Since

   Dec. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV CMV-DIO-(mCherry-U6)-shRNA(anti-Crh)

   Plasmid

   #214732

   Purpose

   Expresses shRNA against CRH, marked with mCherry, in infected and cre positive cells

   Depositor

   Félix Leroy

   Article

   de Leon Reyes et al Cell. 2023 Sep 14;186(19):4152-4171.e31.

   Insert

   anti-Crh shRNA / mCherry
   

   Use

   AAV

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3.1-ADGRL3-Flag

   Plasmid

   #201218

   Purpose

   pCDNA3.1 with kozak (GCC), ADGRL3 and C-terminal Flag

   Depositor

   Jonathan Javitch

   Article

   Perry-Hauser et al J Biol Chem. 2022 Dec;298(12):102594. do

   Insert

   ADGRL3 (Adgrl3 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFBD-Akt13C(S473D)

   Plasmid

   #86577

   Purpose

   Expresses cleavable human full-length Akt1(S473D) phosphomimetic

   Depositor

   Ivan Yudushkin, Thomas Leonard

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Tags

   10xHis

   Expression

   Insect

   Mutation

   3C cleavage site inserted at pos. 130; S473D phos…

   Available Since

   April 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-5X-3-SARS-CoV-2-3CL

   Plasmid

   #168457

   Purpose

   Bacterial codon optimized expression vector for SARS-CoV-2 3CL protease.

   Depositor

   David Ho, Alejandro Chavez, Sho Iketani

   Article

   Iketani et al Nat Commun. 2021 Apr 1;12(1):2016. doi:

   Insert

   SARS-CoV-2 3CL protease (ORF1ab Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   March 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pHBS1449 SED1-Lenti

   Plasmid

   #180824

   Purpose

   SED1 biosensor for stable integration and expression in mammalian cells

   Depositor

   Rajat Rohatgi

   Article

   Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

   Insert

   SED1 osmosensor
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST-HisMBP-SED1

   Plasmid

   #178191

   Purpose

   SED1 biosensor for expression in Escherichia coli

   Depositor

   Cesar Cuevas-Velazquez, Jose Dinneny

   Article

   Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

   Insert

   SED1 biosensor
   

   Tags

   6xHis-MBP

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT7-7 aSyn C141

    Plasmid

    #108866

    Purpose

    Expresses human WT alpha synuclein with a C-terminal cysteine

    Depositor

    Gabriele Kaminski Schierle

    Article

    Stephens et al bioRxiv 2022.10.04.510646

    Insert

    alpha synuclein (SNCA Human)
    

    Expression

    Bacterial

    Mutation

    C141 mutation to allow C-terminal maleimide dye l…

    Promoter

    T7

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRSFDuet-1_VNp-mNeongreen-StefinA

    Plasmid

    #182419

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-mNeongreen-stefinA fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-mNeongreen-stefin (CSTA Human)
    

    Tags

    Vesicle Nucleating peptide (VNp) & mNeongreen

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-FLAG-Grb10-WT

    Plasmid

    #74501

    Purpose

    Overexpression of Grb10 in mammalian cells

    Depositor

    Lars Rönnstrand

    Article

    Kazi et al Mol Oncol. 2013 Jun;7(3):402-18. doi: 10

    Insert

    Grb10 (GRB10 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    L358S, V522A, and V524L

    Promoter

    CMV

    Available Since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

13. AAV-SYP1-miniSOG-T2A-mCherry

    Plasmid

    #50972

    Purpose

    AAV2 transfer vector containing the InSynC (SYP1-miniSOG-T2A-mCherry) construct

    Depositor

    Roger Tsien

    Article

    Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

    Has Service

    AAV8

    Insert

    Synaptophysin-miniSOG-T2A-mCherry (Syp Rat)
    

    Use

    AAV

    Tags

    T2A-mCherry and miniSOG

    Promoter

    human synapsin promoter

    Available Since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRetroX-PTuner DD-M.EcoGII-v5-Telomeric repeat-binding-factor1

    Plasmid

    #122084

    Purpose

    Express M.EcoGII fused to TRF1 with detabilization domain - inducible

    Depositor

    Laure Crabbe

    Article

    Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

    Insert

    DD-linker-M.EcoGII-V5-TERF1
    

    Use

    Retroviral

    Tags

    V5 tag on Nter of TERF1

    Expression

    Bacterial and Mammalian

    Available Since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. TCR012H

    Plasmid

    #233454

    Purpose

    Expresses ncHLAp-reactive T cell receptor (TCR012H)

    Depositor

    William Freed-Pastor

    Article

    Ely et al Science. 2025. May 8; 388(6747)

    Insert

    TCR012H
    

    Use

    Lentiviral

    Available Since

    May 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. 3XHalo_GFP_TRF1#1

    Plasmid

    #164644

    Purpose

    tag TRF1 with GFP and Halo

    Depositor

    Huaiying Zhang, Michael Lampson

    Article

    Zhang et al Mol Biol Cell. 2020 Aug 15;31(18):2048-2

    Insert

    TRF1 (TERF1 Human)
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYFP-ErbB2

    Plasmid

    #66948

    Purpose

    Mammalian expression of rat ErbB2 tagged with YFP

    Depositor

    Bo van_Deurs, Mads Lerdrup

    Article

    Lerdrup et al Mol Biol Cell. 2007 Sep;18(9):3656-66. E

    Insert

    ErbB2 (Erbb2 Rat)
    

    Tags

    6xHis, V5 Tag, and YFP

    Expression

    Mammalian

    Mutation

    (See depositor comment below on mutations A24T an…

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pCMV_Anc689-NG-nCas9

    Plasmid

    #163527

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    Anc689-NG-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

    Available Since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBactin-Tubg1-mEGFP

    Plasmid

    #196870

    Purpose

    Expression of the gamma-tubulin fused to enhanced (E)-GFP

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Tubg1-mEGFP (Tubg1 Mouse)
    

    Expression

    Mammalian

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR_P2R-P3_R2-miniTurbo-mVenus-STOP-L3

    Plasmid

    #127356

    Purpose

    gateway entry vector for making C-terminal miniTurbo-mVenus fusion with nuclear and non-nuclear proteins

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    miniTurbo (BirA mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, V5, and mVenus

    Mutation

    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

    Promoter

    no promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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