We narrowed to 7,168 results for: GFP expression plasmids

1. XZ054 (PB)SFFV-LIDARsensor (hygR)

   Plasmid

   #233419

   Purpose

   PiggyBac donor plasmid with SFFV driven LIDAR reporter RNA (mCherry as marker, EGFP as output) for stable integration

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   mCherry-SGc(stem-1(3xMS2))cSG-EGFP
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAID5.3-C

   Plasmid

   #145813

   Purpose

   An all-in-one bicistronic plasmid for controlling protein degradation by AID technology

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Pharmaceuticals (Basel). 2020 May 23;13(

   Type

   Empty backbone

   Tags

   mAID

   Expression

   Mammalian

   Available Since

   June 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAID5.1-C

   Plasmid

   #145809

   Purpose

   An all-in-one bicistronic plasmid for controlling protein degradation by AID technology

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Pharmaceuticals (Basel). 2020 May 23;13(

   Type

   Empty backbone

   Tags

   mAID

   Expression

   Mammalian

   Available Since

   June 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAID5.1-N

   Plasmid

   #145808

   Purpose

   An all-in-one bicistronic plasmid for controlling protein degradation by AID technology

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Pharmaceuticals (Basel). 2020 May 23;13(

   Type

   Empty backbone

   Tags

   mAID

   Expression

   Mammalian

   Available Since

   June 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAID5.3-N

   Plasmid

   #145812

   Purpose

   An all-in-one bicistronic plasmid for controlling protein degradation by AID technology

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Pharmaceuticals (Basel). 2020 May 23;13(

   Type

   Empty backbone

   Tags

   mAID

   Expression

   Mammalian

   Available Since

   June 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. mGFAP-Halo

   Plasmid

   #169474

   Purpose

   the host vector was obtained by substitution of the EGFP tag from a pEGFP-N3-mGFAP plasmid for a HaloTag using BamHI and NotI restriction sites

   Depositor

   Federico Herrera

   Article

   Letra-Vilela et al Experimental Results (2020), Volume 1, e

   Insert

   GFAP (Gfap Mouse)
   

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Changed R 236 to H

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWN10042

   Plasmid

   #89052

   Purpose

   GF-ori-FP plasmid for GFxFP assay

   Depositor

   Ervin Welker

   Article

   Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

   Inserts

   EGFP part 1
   EGFP part 2
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CAG and none

   Available Since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEG302-SunTag-FWAgRNA-4-22aa-TET1cd

   Plasmid

   #115344

   Purpose

   SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoter

   Depositor

   Steven Jacobsen

   Insert

   gRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   March 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJP_1node

   Plasmid

   #230984

   Purpose

   pBAD promoter controls the expression of sfGFP. In the presence of VVD-AraC, blue light and L-arabinose can be used to activate mCitrine expression.

   Depositor

   Yolanda Schaerli

   Article

   Hollo et al Mol Syst Biol. 2025 Sep 29. doi: 10.1038

   Insert

   mCitrine
   

   Expression

   Bacterial

   Available Since

   Sept. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. LifeAct-14 (LA-14)

    Plasmid

    #158750

    Purpose

    Lentiviral plasmid for expression of shorter LifeAct variant (up till 14 amino acids) fused with carboxy terminus eGFP in mammalian cells.

    Depositor

    Minhajuddin Sirajuddin

    Article

    Kumari et al EMBO J. 2020 Jun 22:e104006. doi: 10.152

    Insert

    LifeAct-14
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Deletion mutation for last three amino acids from…

    Promoter

    CMV immediate early

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. CaMK2rep-S603A

    Plasmid

    #239623

    Purpose

    Inactive CaMKII activity reporter. The plasmid contains sGFP2 fluorescent protein, 3xmyc tags and double mutated phospho-site 3 from rat Synapsin-1a.

    Depositor

    F Javier Díez Guerra

    Article

    Martinez-Blanco et al Anal Chem. 2025 Sep 23;97(37):20275-2029

    Insert

    SpotNES-sGFP2-SynP3-3xmyc (Syn1 Synthetic, Rat)
    

    Tags

    Spot, myc, and sGFP2

    Expression

    Mammalian

    Mutation

    changed Serine 603 to Alanine

    Promoter

    CMV

    Available Since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV_ACTB CRISPIE

    Plasmid

    #172853

    Purpose

    Actb sgRNA & DRS-2 sgRNA expression under U6. CRISPIE donor mEGFP phase (0-0), excised by DRS-1 or DRS-2 (with SpCas9). Also expresses mRuby3 under the CBA promotor. See Zhong et al, eLife 2021.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Inserts

    Actb intron 1 sgRNA
    DRS-2 sgRNA
    CRISPIE designer exon (phase 0-0) encoding mEGFP
    mRuby3
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. scFv-GCN4-DNMT3a(R887E)-DNMT3l

    Plasmid

    #154141

    Purpose

    Expresses a higher specificity variant of scFv-GCN4-DNMT3a-DNMT3l, contains R887E mutation in DNMT3a. To be used with the dCas9-SunTag system for targeted DNA methylation.

    Depositor

    Albert Jeltsch

    Article

    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

    Insert

    scFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP
    

    Tags

    HA and sfGFP

    Expression

    Mammalian

    Mutation

    R887E

    Promoter

    SFFV

    Available Since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. miniABEmax (pRZ900)

    Plasmid

    #131311

    Purpose

    CMV promoter expression plasmid for bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (ABEmax with truncation of WT TadA domain).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

    Insert

    bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCBh-T7-SpCas9-P2A-mTagBFP2(RAS3575)

    Plasmid

    #223111

    Purpose

    pCBh Human expression plasmid for SpCas9 with with C-term BPNLS-P2A-mTagBFP2

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9-P2A-mTagBFP2
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-mTagBFP2

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. SECURE miniABEmax-V82G (pJUL1828)

    Plasmid

    #131313

    Purpose

    CMV promoter expression plasmid for bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with V82G mutation).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

    Insert

    bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAP215.rAAV.mU6-sgRNA.Handle.EF1a-mTagBFP2

    Plasmid

    #217635

    Purpose

    AAV backbone for sgRNA expression. Contains a Lox-flanked handle sequence for Cre-dependent PCR amplification of sgRNAs. Protospacer is cloned between BstXI and BlpI.

    Depositor

    Martin Kampmann

    Article

    Ramani et al Nat Neurosci. 2025 Aug 22. doi: 10.1038/

    Insert

    mU6-sgRNA, Handle sequence, EF1a-mTagBFP2
    

    Use

    AAV

    Promoter

    EF1alpha and mU6

    Available Since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. mARGAna (cassette 2)

    Plasmid

    #191342

    Purpose

    Second-generation mammalian acoustic reporter gene derived from Anabaena

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    mARGAna (cassette 2)
    

    Expression

    Mammalian

    Promoter

    TRE3GV

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. mARGAna (cassette 1)

    Plasmid

    #191341

    Purpose

    Second-generation mammalian acoustic reporter gene derived from Anabaena

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    mARGAna (cassette 1)
    

    Expression

    Mammalian

    Promoter

    TRE3GV

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. FHUG+W (B2)

    Plasmid

    #74011

    Purpose

    lentiviral expression of EGFP and shRNA (from H1 promoter, empty)

    Depositor

    Robert Malenka, Oliver Schluter, Weifeng Xu

    Article

    Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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