We narrowed to 7,184 results for: RAP

1. pAAV-hSyn-GRAB_DA1h

   Plasmid

   #113050

   Purpose

   express the genetically-encoded fluorescent dopamine(DA) sensor GRAB_DA1h in neurons

   Depositor

   Yulong Li

   Article

   Sun et al Cell. 2018 Jul 12;174(2):481-496.e19. do

   Has Service

   AAV Retrograde and AAV9

   Insert

   GPCR activation based DA sensor GRAB_DA1h (DRD2 Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330-TP53-2

   Plasmid

   #121918

   Purpose

   Encodes sgRNA targeting exon 9 of TP53

   Depositor

   William Hahn

   Article

   Howard et al Cancer Res. 2019 Feb 12. pii: 0008-5472.

   Insert

   TP53 (TP53 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCag FlpO-2A-Cre EV

   Plasmid

   #129419

   Purpose

   episomal expression of FlpO and Cre recombinases

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   FlpO-2A-Cre
   

   Use

   Cre/Lox and Unspecified; Episomal expression vect…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/Chick β-actin (CAG)

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. LgBiT-hACE2

   Plasmid

   #173431

   Purpose

   protein expression plasmid of LgBiT-hACE2-IgG1 Fc

   Depositor

   David Nemazee

   Article

   Huang et al J Immunol. 2021 Jul 1;207(1):344-351. do

   Insert

   LgBiT-hACE2-IgG1 Fc (ACE2 Human)
   

   Use

   Luciferase

   Tags

   IgG1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL3Basic-ME.2/ApoEpromoter

   Plasmid

   #51436

   Purpose

   This plasmid is a luciferase-based reporter construct to measure the activity of the ApoE promoter fused to ME.2 enhancer

   Depositor

   Sohail Tavazoie

   Article

   Pencheva et al Cell. 2014 Feb 27;156(5):986-1001. doi:

   Insert

   APOE apolipoprotein E (APOE, ME2 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   The ME.2 enhancer is fused upstream of the ApoE g…

   Promoter

   Available since

   Feb. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSIN-hSNCA-NE

   Plasmid

   #102366

   Purpose

   Lentiviral overexpression of synuclein alpha

   Depositor

   Shu Leong Ho

   Article

   Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

   Insert

   synuclein alpha (SNCA Human)
   

   Use

   Lentiviral

   Tags

   NE

   Expression

   Mammalian

   Mutation

   A silent mutation (333bp downstream of ATG) was i…

   Promoter

   EF-1a

   Available since

   April 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIG-804_HA-GD2-28z_CAR_BATF-TFAP4

   Plasmid

   #207491

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   BATF-TFAP4, HA-GD2-28z_CAR (BATF Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-Ef1a-DIO-PPO-Venus

   Plasmid

   #139505

   Purpose

   AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.

   Depositor

   Michael Bruchas

   Article

   Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

   Has Service

   AAV8 and AAV9

   Insert

   parapinopsin
   

   Use

   AAV

   Tags

   Venus tag following 3x Ala linker

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFastBac1-pGC-A

   Plasmid

   #186626

   Purpose

   pFastBac1 (baculovirus polyhedrin promoter) encoding HA signal peptide, TEV-protease cleavable His10-tag, and full-length human pGC-A (NP_000897.3 amino acids 33-1061; expression-optimized DNA)

   Depositor

   Debra Hansen

   Article

   Zhang et al Sci Rep. 2022 Jul 12;12(1):11824. doi: 1

   Insert

   NPR1 (NPR1 Human)
   

   Use

   Tags

   hemagglutinin (HA) signal peptide + His10-tag + T…

   Expression

   Insect

   Mutation

   deleted amino acids 1-32

   Promoter

   polyhedring

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL3Basic_ME.1/ApoEpromoter

    Plasmid

    #51435

    Purpose

    This plasmid is a luciferase-based reporter construct to measure the activity of the ApoE promoter fused to ME.1 enhancer

    Depositor

    Sohail Tavazoie

    Article

    Pencheva et al Cell. 2014 Feb 27;156(5):986-1001. doi:

    Insert

    ME.1+ApoE promoter -980/+(93 bp.) (APOE, ME1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    ME.1 enhancer is fused upstream of ApoE promoter …

    Promoter

    Available since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pKLV2-U6gRNA3(BbsI)-PGKpuro2ABFP

    Plasmid

    #67990

    Purpose

    CRISPR gRNA expression vector with the conventional scaffold and puro/BFP markers without WPRE

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette with the conventional scaffold, PGKpuro2ABFP cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBK2045-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2(CMV-gRNA1)

    Plasmid

    #223165

    Purpose

    2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and gRNA1 targeting CMV

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB-MECP2 (MECP2 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 204-310 (MECP2)

    Promoter

    EF1a

    Available since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRISPaint-mNeon-PuroR [M1G]

    Plasmid

    #171800

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-T2A-PuroR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Promoter

    Available since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKLV2-U6gRNA5(gBFP)-PGKGFP2ABFP-W

    Plasmid

    #67984

    Purpose

    Cas9 activity reporter with GFP and BFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Inserts

    U6gRNA cassette, PGKGFP2ABFP cassette, WPRE
    Guide RNA targeting modified BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. mRFP-FKBP12-5ptpase domain

    Plasmid

    #67516

    Purpose

    Recruitable human type IV 5-phosphatase domain (214-644)

    Depositor

    Tamas Balla

    Article

    Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

    Insert

    FKBP12-5ptpase enzyme (INPP5E, FKBP1A Human)
    

    Use

    Tags

    mRFP

    Expression

    Mutation

    INPP5E has C641A to destroy the C-terminal CAAX d…

    Promoter

    Available since

    Sept. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1-NLS-myc-dL5-2xG4S-mCer3

    Plasmid

    #73205

    Purpose

    Expresses myc-dL5(E52D)-mCer3 fusion protein in nuclei of mammalian cells. (MBIC5, dL5**, FAP)

    Depositor

    Marcel Bruchez

    Article

    Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46

    Insert

    NLS-myc-dL5-2XG4S-mCer3 (MYC Human, Synthetic)
    

    Use

    Tags

    The FAP and mCerulean3 are fused with 2 copies of…

    Expression

    Mammalian

    Mutation

    The dL5** FAP is E50D, L89S, also known as E52D/L…

    Promoter

    CMV

    Available since

    March 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRISPaint-mNeon-BlastR [M1G]

    Plasmid

    #171805

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and blasticidin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-T2A-BlastR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    BlastR: Changed Methionin 1 to Glycine.

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO.1-TRC.mKO2_shB3GNT5.1

    Plasmid

    #110325

    Purpose

    TRCN0000034809 (Target GCCTATGTAATCTCCGGTGAT), silence human B3GNT5 gene and express monomeric Kusabira-Orange2

    Depositor

    Sandra Martha Gomes Dias

    Article

    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

    Insert

    B3GNT5 (B3GNT5 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RNA polymerase III promoter for human U6 snRNA fo…

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIG-835_NY-ESO-1_TCR_FAS/MyD88

    Plasmid

    #207498

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    FAS/MyD88, NY-ESO-1_TCR (FAS Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-CAG-DIO-PPO-Venus

    Plasmid

    #139504

    Purpose

    AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the CAG promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.

    Depositor

    Michael Bruchas

    Article

    Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

    Has Service

    AAV5

    Insert

    parapinopsin
    

    Use

    AAV

    Tags

    Venus tag following 3x Ala linker

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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