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pMT1219

Plasmid

#228301

Purpose

2,4-Diacetylphloroglucinol (DAPG)-regulatable expression of SARS-CoV-2 RBD omicron variant

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

MFalpha(2xAdv)-SARS CoV-2 receptor binding domain (RBD)-His (S Severe acute respiratory syndrome-related coronavirus 2, Synthetic)

Use

Synthetic Biology

Tags

GGG linker followed by His6 and MF_ secretion sig…

Expression

Yeast

Mutation

G339D, S371L, S373P, S375F, K417N, N440K, G446S, …

Promoter

2,4-Diacetylphloroglucinol-regulatable synthetic …

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2.2-h7SKgRNA5(SMARCA4(43))-hU6gRNA5(AAVS1)-PGKpuroBFP-W

Plasmid

#200503

Purpose

Lentiviral vector expressing gRNA targeting human SMARCA4 and AAVS1

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

SMARCA4(43), AAVS1 (SMARCA4, AAVS1 Human)

Use

Lentiviral

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2.2-h7SKgRNA5(SMARCA4(43))-hU6gRNA5(SMARCA2(47))-PGKpuroBFP-W

Plasmid

#200505

Purpose

Lentiviral vector expressing gRNA targeting human SMARCA4 and SMARCA2

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

SMARCA4(43), SMARCA2(47) (SMARCA4, SMARCA2 Human)

Use

Lentiviral

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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PB-TAC-OSKM

Plasmid

#80481

Purpose

piggyBac transposon for dox-inducible expression of the OSKM polycistronic reprogramming cassette (with mCherry)

Depositor

Knut Woltjen

Article

Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

Insert

OSKM (Pou5f1, Sox2, Klf4, Myc Mouse)

Use

Piggybac transposon

Expression

Mammalian

Promoter

tetO

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-PDGFRb-HA

Plasmid

#204350

Purpose

Expresses wild-type PDGFRb gene. Used for DNA delivery using Adeno-associated Virus.

Depositor

Hirofumi Nakatomi

Article

Shima et al Sci Transl Med. 2023 Jun 14;15(700):eabq

Insert

Platelet derived growth factor receptor beta (PDGFRB) (PDGFRB Human)

Use

AAV

Tags

HA

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS-PDGFRb559-562del-IRES2-EGFP

Plasmid

#204355

Purpose

Expresses a mutant PDGFRb gene (559-562 deletion).

Depositor

Hirofumi Nakatomi

Article

Shima et al Sci Transl Med. 2023 Jun 14;15(700):eabq

Insert

Platelet derived growth factor receptor beta (PDGFRB) (PDGFRB Human)

Mutation

559-562 deletion

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-PDGFRb559-562del-HA

Plasmid

#204351

Purpose

Expresses a mutant PDGFRb gene (559-562 deletion). Used for DNA delivery using Adeno-associated Virus.

Depositor

Hirofumi Nakatomi

Article

Shima et al Sci Transl Med. 2023 Jun 14;15(700):eabq

Insert

Platelet derived growth factor receptor beta (PDGFRB) (PDGFRB Human)

Use

AAV

Tags

HA

Mutation

559-562 deletion

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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PB-TAC-MKOS

Plasmid

#80484

Purpose

piggyBac transposon for dox-inducible expression of the MKOS polycistronic reprogramming cassette (with mCherry)

Depositor

Knut Woltjen

Article

Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

Insert

MKOS (Myc, Klf4, Pou5f1, Sox2 Mouse)

Use

Piggybac transposon

Expression

Mammalian

Promoter

tetO

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2.2-h7SKgRNA5(ARID1A(44))-hU6gRNA5(ARID1B(21))-PGKpuroBFP-W

Plasmid

#200507

Purpose

Lentiviral vector expressing gRNA targeting human ARID1A and ARID1B

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

ARID1A(44), ARID1B(21) (ARID1A, ARID1B Human)

Use

Lentiviral

Available Since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2.2-h7SKgRNA5(ARID1A(44))-hU6gRNA5(ARID1B(23))-PGKpuroBFP-W

Plasmid

#200508

Purpose

Lentiviral vector expressing gRNA targeting human ARID1A and ARID1B

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

ARID1A(44), ARID1B(23) (ARID1A, ARID1B Human)

Use

Lentiviral

Available Since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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PB-TAC-OKMS

Plasmid

#80480

Purpose

piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry)

Depositor

Knut Woltjen

Article

Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

Insert

OKMS (Pou5f1, Klf4, Myc, Sox2 Mouse)

Use

Piggybac transposon

Expression

Mammalian

Mutation

Klf4 [10-483]

Promoter

tetO

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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PB-TAC-OK+9MS

Plasmid

#80482

Purpose

piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry)

Depositor

Knut Woltjen

Article

Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

Insert

OK+9MS (Pou5f1, Klf4, Myc, Sox2 Mouse)

Use

Piggybac transposon

Expression

Mammalian

Mutation

Klf4 [1-483]

Promoter

tetO

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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PB-TAC-EB-C5

Plasmid

#80485

Purpose

piggyBac transposon for dox-inducible expression of the EB-C5 polycistronic reprogramming cassette (with mCherry)

Depositor

Knut Woltjen

Article

Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

Insert

lin-28 homolog A (C. elegans) (Pou5f1, Sox2, Klf4, Myc, Lin28a Mouse)

Use

Piggybac transposon

Expression

Mammalian

Promoter

tetO

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pZ:F3-CAGGS GPHTK-F

Plasmid

#112666

Purpose

Genome engineering donor vector for creating master cell lines suitable for FLPe recombinase-mediated cassette exchange (RMCE) in the AAVS1 locus

Depositor

Catherine Verfaillie

Article

Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-

Insert

eGFP

Use

Gene targeting vector for integration at the s1 s…

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pSLIK_hygro_3xflag_NRF2(RR)

Plasmid

#136535

Purpose

Lentiviral expression vector for an inducible 3xflag-tagged NRF2(a1584c_a1586t_a1589g)

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

NRF2(1584A>C,1586A>T,1589A>G) (NFE2L2 Human)

Use

Lentiviral; Destinatioin vector for gateway cloni…

Tags

3x FLAG

Expression

Mammalian

Mutation

This plasmid is developed by mutating 3 base pair…

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEN_TTmiRC2_3xflag_NRF2(RR)

Plasmid

#136522

Purpose

Used as a donor vector containing N-term 3xFLAG to clone into pSLIK

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

NRF2(1584A>C,1586A>T,1589A>G) (NFE2L2 Human)

Use

Entry vector for gateway cloning

Tags

3x FLAG

Mutation

This plasmid is developed by mutating 3 base pair…

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3_N-DYK_MTHFD1-NLS

Plasmid

#133023

Purpose

NLS sequence was fused to MTHFD1-wild type through site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs)

Depositor

Stefan Kubicek

Article

Sdelci et al Nat Genet. 2019 Jun;51(6):990-998. doi:

Insert

MTHFD1 (MTHFD1 Human)

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3_N-DYK_MTHFD1-NES

Plasmid

#133022

Purpose

NES sequence was fused to MTHFD1-wild type through site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs)

Depositor

Stefan Kubicek

Article

Sdelci et al Nat Genet. 2019 Jun;51(6):990-998. doi:

Insert

MTHFD1 (MTHFD1 Human)

Tags

NES

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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PHyTc Notch4HA

Plasmid

#21469

Depositor

Jan Kitajewski

Insert

Notch4 (Notch4 Mouse)

Use

Retroviral

Tags

HA

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pHyTc Notch4

Plasmid

#21468

Depositor

Jan Kitajewski

Insert

Notch4 (Notch4 Mouse)

Use

Retroviral

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only