We narrowed to 37,896 results for: lat
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Plasmid#157791PurposeEcoRV digestion produces 12x601 chromatin assembly construct with 45 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomesDepositorInsert12x601_45bp linker
ExpressionBacterialAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132D
Plasmid#69288PurposeGolden Gate entry vector; 1st gRNA under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRL-TK 4x wt
Plasmid#11313DepositorInsert4 bulged binding sites for CXCR4 siRNA antisense
UseLuciferaseTagsRr-lucAvailable sinceFeb. 15, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-DRP1 K38A
Plasmid#232350PurposeExpresses K38A variant of human DRP1 with mCherry tagDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDRP1 (DNM1L Human)
TagsmCherryExpressionMammalianMutationK38APromoterCMVAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBK2044-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2
Plasmid#223164Purpose2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and empty gRNA scaffoldDepositorInsertKRAB-MECP2 (MECP2 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 204-310 (MECP2)PromoterEF1aAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJAT7
Plasmid#204292PurposeFor inserting a phiC31 attP site. HDR integrant is marked with removable 3XP3-DsRed cassette, which is expressed in eyes. DsRed difficult to see in wild type eyes if using LeicaM165 FC microscope.DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert3XP3-DsRed
UseCRISPRPromoter3XP3Available sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJAT30
Plasmid#204294PurposeFor marking CRISPR HDR integrant with DsRed expressed in flight muscles. Second U6 promoter for using two gRNAs.DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMHC-DsRed
UseCRISPRPromoterMHCAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
vYM082_PB-pEF1s(min)-3xNLS-Citrine-T2A-iaaH(Hygro)
Plasmid#160044PurposeFor expressing iaaH to convert auxin precursors (IAM or NAM) to auxin (IAA or NAA)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertiaaH (iaaH )
UseSynthetic BiologyTags3xNLS-CitrineExpressionMammalianPromoterpEF1sAvailable sinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
PH-Btk-mRuby2
Plasmid#124657PurposePI(3,4,5)P3 probe. PH domain of BTK fused with mRuby2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBTK (BTK Human)
TagsmRuby2ExpressionMammalianMutationcontains only the PH domain of Bruton tyrosine ki…Available sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hA3A-BE3-Y130F
Plasmid#113428PurposeExpresses hA3A-BE3-Y130F in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthA3A-BE3-Y130F (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)
UseCRISPRExpressionMammalianMutationhAPOBEC3A_Y130FPromoterCMVAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKan-PRFA1-9myc-AID*(N)
Plasmid#99528PurposeN-terminal 9myc-AID* degron cassette with RFA1 promoter and KanR marker for S. cerevisiaeDepositorInsertIAA17(71-117) (AXR3 Mustard Weed)
UsePcr template for yeast tagging cassetteTags9xMycPromoterRFA1Available sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST-hMeCP2-GFP-R270X
Plasmid#48079PurposeMammalian expression of GFP tagged MeCP2-R270XDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMethyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
TagsGFPExpressionMammalianMutationexpresses AT-Hook R270XPromoterCMVAvailable sinceApril 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL3-1574/+120
Plasmid#35539DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAUseLuciferaseTagsluciferasePromoternoneAvailable sinceJune 25, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMA_Int
Plasmid#110096PurposeE. coli replicative vector which contains the mycophage integrase gene required for the attP/attB integration. Is co-transformed in trans together with pFLAG_attP, but cannot replicate in mycobacteria, and is thus lost shortly after electroporation.DepositorInsertmycophage integrase
UseRequired for transformation of attb integrating v…Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SIRT5.1
Plasmid#13738DepositorAvailable sinceFeb. 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
pROP-PP-FBFP
Plasmid#122137PurposeFMN-based fluorescent protein Reporter plasmid for B-Rex contain perfect operator sequence (anaerobic purpose)DepositorInsertFBFP
ExpressionBacterialPromoterJ23101Available sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV hU6-gRNA hUbC-dSaCas9-KRAB-T2A-Thy1.1
Plasmid#194278PurposeExpresses gRNA and dSaCas9-KRAB from lentiviral vectorDepositorInsertshumanized dSaCas9 KRAB T2A Thy1.1
gRNA
UseCRISPR and LentiviralTagsHAExpressionMammalianPromoterhU6 and hUbCAvailable sinceJan. 13, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
vYM073_PB-pEF-osTIR1-T2A-mCherry-AID-Flag-BlastR
Plasmid#160042PurposeFor the auxin receiver that report on auxin.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsosTIR1
BlastR
UseLuciferaseTagsmAID and mCherryExpressionMammalianAvailable sinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-huTET1CD-T2A-mCherry(PX458)
Plasmid#129027Purposetargeted DNA demethylation, expression of dCas9-huTET1CD-T2A-mCherry and cloning backbone for sgRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9-huTET1CD, SgRNA cloning site
TagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A)PromoterCbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)Available sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEB2-mCherry2-L
Plasmid#104003PurposePlasmid encoding mCherry2-LDepositorInsertmCherry2-L
UseLow copyExpressionBacterialMutationMutations relative to DsRed (MSKGEE NNLA IIKEF… V…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only