We narrowed to 9,514 results for: try

1. pREG(R1-R3) #BGV141

   Plasmid

   #48958

   Purpose

   Retroviral Destination vector containing AttR1-AttR3 site. For use with three-way LR reaction with cDNA in entry vector and a marker in pBEG R2-L3 plasmids (module 2 and module 3 plasmids).

   Depositor

   David Dankort

   Article

   Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

   Type

   Empty backbone

   Use

   Retroviral

   Promoter

   CMV

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pENTR4_FLAG/HA_FUS_His6

   Plasmid

   #26365

   Depositor

   Thomas Tuschl

   Article

   Spitzer et al Genes Chromosomes Cancer. 2011 May . 50(

   Insert

   FUS (FUS Human)
   

   Use

   Entry vector

   Tags

   FLAG/HA and His6

   Mutation

   delta 527 (stop codon)

   Available Since

   Oct. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pEN-R2-mCsfGFP-L3

   Plasmid

   #178602

   Purpose

   It can be used to study protein turnover in living plant cells of Arabidopsis (Arabidopsis thaliana) and Nicotiana benthamiana.

   Depositor

   Frederica Theodoulou

   Article

   Zhang et al Plant Physiol. 2019 Jun;180(2):718-731.

   Insert

   Tandem fluorescent protein timers (tFTs)
   

   Use

   Entry vector for gateway cloning

   Tags

   mCherry superfolder GFP

   Available Since

   Dec. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ256

   Plasmid

   #124312

   Purpose

   Gateway entry vector with PmCDA1 and pcoCas9D10A for C-T base editing

   Depositor

   Yiping Qi

   Article

   Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

   Insert

   pcoCas9D10A-PmCDA1-UGI
   

   Use

   CRISPR

   Available Since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR-p27(kip1)

   Plasmid

   #50026

   Purpose

   pENTR vector for human cyclin-dependent kinase inhibitor 1B

   Depositor

   Jamie Davies

   Article

   Cachat et al J Biol Eng. 2014 Nov 19;8(1):26. eCollec

   Insert

   p27(kip1) (CDKN1B Human)
   

   Use

   Entry vector

   Available Since

   Dec. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pCheck2 Dest (R1-R2) #BGV129

   Plasmid

   #48955

   Purpose

   Destination dual luciferase reporter plasmid for checking effectiveness of shRNA. Compatible with standard AttL1-AttL2 Gateway Entry vectors.

   Depositor

   David Dankort

   Article

   Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

   Type

   Empty backbone

   Use

   Luciferase; Gateway cloning vector

   Promoter

   SV40

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pDONR223-SLC7A11 sh926R

   Plasmid

   #118701

   Purpose

   entry clone for SLC7A11 insensitive to hairpin TRCN0000288926

   Depositor

   William Kaelin

   Article

   Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

   Insert

   SLC7A11 (SLC7A11 Human)
   

   Expression

   Mammalian

   Mutation

   wt , SLC7A11 sh926R

   Available Since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-ERBB4 (R544W)

   Plasmid

   #29531

   Depositor

   Yardena Samuels

   Article

   Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

   Insert

   ERBB4 (ERBB4 Human)
   

   Expression

   Mammalian

   Mutation

   changed arginine 544 to tryptophan

   Available Since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

9. KTK_001

   Plasmid

   #180517

   Purpose

   Empty backbone for creation of entry parts for the KTK, contains sfGFP dropout

   Depositor

   Tom Ellis

   Article

   Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   April 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-M2-TLR2-V660N

    Plasmid

    #12292

    Depositor

    Bruce Beutler

    Article

    Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    TLR2 (Tlr2 Mouse)
    

    Tags

    FLAG (FLAG-M2)

    Expression

    Mammalian

    Mutation

    M82I, H202Y and V660N; signal peptide (aa1-23) re…

    Available Since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pCMV-M2-TLR2-P681H

    Plasmid

    #12290

    Depositor

    Bruce Beutler

    Article

    Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    TLR2 (Tlr2 Mouse)
    

    Tags

    FLAG (FLAG-M2)

    Expression

    Mammalian

    Mutation

    A74V, M82I, H202Y and P681H; signal peptide (aa1-…

    Available Since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pLJM60 FLAG NUFIP1 W40A

    Plasmid

    #110951

    Purpose

    expresses mammalian FLAG-NUFIP1 W40A

    Depositor

    David Sabatini

    Article

    Wyant et al Science. 2018 May 18;360(6390):751-758.

    Insert

    NUFIP1 W40A (NUFIP1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Tryptophan 40 to alanine

    Promoter

    CMV

    Available Since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYPQ133D2.0

    Plasmid

    #99894

    Purpose

    Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    OsU3

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPQ131D2.0

    Plasmid

    #100044

    Purpose

    Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    OsU3

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. C513-E07: attB5r-<p10p-polyhedrinp>-attB1r

    Plasmid

    #162949

    Purpose

    Gateway attB5r/attB1r entry clone containing divergent AcMNPV polyhedrin and p10 promoters, for use in construction of polycistronic baculovirus expression vectors

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    dual baculovirus promoter cassette (AcMNPV p10 and polyhedrin)
    

    Use

    Synthetic Biology

    Available Since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYPQ292 (AaCas12b)-D570A-TV

    Plasmid

    #136380

    Purpose

    CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AaCas12b (D570A)-TV
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D570A

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pG418R-GMRWhite

    Plasmid

    #165883

    Purpose

    G418 resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Transgenesis Ready Selection Vector
    

    Use

    Synthetic Biology

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBP-ORF

    Plasmid

    #72949

    Purpose

    Entry vector for ORF's - PCR, gene synthesis or sub-clone using NdeI/BamHI. Alternatively use BsaI with CATA and TCGA fusion sites. Stop codon must be included upstream of chosen 3' restriction site

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 0 - ORFs
    

    Use

    Synthetic Biology

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEnt R3L2 TetO(sh) mCh-Rs1-2

    Plasmid

    #24417

    Depositor

    Edward Hsiao

    Article

    Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

    Insert

    Rs1
    

    Use

    Entry vector

    Tags

    FLAG and mCherry

    Mutation

    Human 5HT4B receptor with D100A mutation, generat…

    Available Since

    March 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCI-Flag-NEDD4L∆WW2

    Plasmid

    #166708

    Purpose

    expresses NEDD4L with inactivating mutation in second WW domain in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

    Insert

    NEDD4L W453A
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed tryptophan 453 to alanine

    Promoter

    CMV promoter

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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