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762 results

Showing: 621 - 640 of 762 results

  1. pMH005_phiC31_neo-core-5xTetO-pEF-H2B-mCitrine-core-pRSV-H2B-mCherry

    Plasmid
    #179433
    Purpose
    Dual fluorescent reporter construct with double core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration
    Depositor
    Lacra Bintu
    Article
    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1
    Insert
    coreHS4-TRE-cit-coreHS4-mch (DC)
    Expression
    Mammalian
    Promoter
    pEF alpha, pRSV
    Available Since
    May 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    179433-plasmid-map-sequence-id-362558

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  3. pMH011_AAVS1_puro-pA-9xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry

    Plasmid
    #179434
    Purpose
    Dual fluorescent reporter construct with single HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus
    Depositor
    Lacra Bintu
    Article
    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1
    Insert
    TRE-cit-HS4-mch (SH)
    Expression
    Mammalian
    Promoter
    pEF alpha, pRSV
    Available Since
    March 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    179434-plasmid-map-sequence-id-355565

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  6. pCRISPaint-mScarlet-F-Ova-BlastR [M1G]

    Plasmid
    #171817
    Purpose
    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and blasticidin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mScarlet-F-Ova-T2A-BlastR
    Use
    Gene tagging
    Mutation
    BlastR: Changed Methionin 1 to Glycine.
    Available Since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    171817-plasmid-map-sequence-id-338126

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  7. pCRISPaint-mNeon-F-Ova-BlastR [M1G]

    Plasmid
    #171808
    Purpose
    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and blasticidin resistance cassette [p.M1G
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mNeonGreen-F-Ova-T2A-BlastR
    Use
    Gene tagging
    Mutation
    BlastR: Changed Methionin 1 to Glycine.
    Available Since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171808-plasmid-map-sequence-id-337980

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  8. pCRISPaint-mNeon-F-Ova-PuroR [M1G]

    Plasmid
    #171804
    Purpose
    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, Ovalbumin CD8+ T cell epitope [AA: 257-264 (SIINFEKL)] and puromycin resistance cassette [p.M1G]
    Depositor
    Michael Hoelzel
    Article
    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.
    Insert
    mNeonGreen-FLAG-Ova-T2A-PuroR
    Use
    Gene tagging
    Mutation
    PuroR: Changed Methionin 1 to Glycine.
    Available Since
    July 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    171804-plasmid-map-sequence-id-337975

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  10. pET15b/GVcl 1-1066 /*TBM

    Plasmid
    #162781
    Purpose
    amplification of Gallus gallus Vcl mutated in Tankyrase Binding Domain II
    Depositor
    Laura Lafon-Hughes
    Article
    et al PeerJ
    Insert
    Gallus gallus vinculin (VCL Chicken)
    Expression
    Bacterial
    Mutation
    changed Gly 454 to Val
    Available Since
    May 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    162781-plasmid-map-sequence-id-321530

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  11. Tol2-GgVCL/WT

    Plasmid
    #162787
    Purpose
    CONTROL_transfection of mammalian cells with WT Vcl under a β-actin promoter, with IRES-GFP allowing identification of transfected cells
    Depositor
    Laura Lafon-Hughes
    Article
    et al PeerJ
    Insert
    Gallus gallus vinculin (VCL Chicken)
    Tags
    IRES-GFP
    Expression
    Bacterial and Mammalian
    Mutation
    WT
    Promoter
    beta actin
    Available Since
    May 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    162787-plasmid-map-sequence-id-327628

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  12. Tol2-GgVCL/*TBM

    Plasmid
    #162790
    Purpose
    MUTATED_transfection of mammalian cells with Vcl mutated in Tankyrase Binding Motif -II (TBM-II) under a β-actin promoter, with IRES-GFP allowing identification of transfected cells
    Depositor
    Laura Lafon-Hughes
    Article
    et al PeerJ
    Insert
    Mutated Gallus gallus vinculin (G454V) (VCL Chicken)
    Tags
    IRES-GFP
    Expression
    Bacterial and Mammalian
    Mutation
    changed Gly 454 to Val
    Promoter
    beta actin
    Available Since
    May 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    162790-plasmid-map-sequence-id-321532

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Showing: 621 - 640 of 762 results