Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 1,212 results for: Ncl

Clear keywords
Showing: 931 - 960 of 1212 results

  9. pMSP1D1T2

    Plasmid
    #97328
    Purpose
    Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include two additional T residues shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.
    Depositor
    Michael Marty
    Article
    Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192
    Insert
    MSP1D1T2
    Tags
    6xHis - TEV
    Expression
    Bacterial
    Mutation
    Addition of two T residues after T25
    Promoter
    T7
    Available Since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97328-plasmid-map-sequence-id-182618

    Enlarge

  10. pET30-GBF-E6C

    Plasmid
    #21698
    Depositor
    James Baleja
    Article
    Liu et al J Mol Biol. 2009 Mar 6. 386(4):1123-37.
    Insert
    Protein G domain B1 fused C-terminal zinc binding domain of human papillomavirus type 16 (residue 79-142) (E6 Human papillomavirus type 16)
    Tags
    Protein G B1 domain
    Expression
    Bacterial
    Mutation
    GB1 (1-56aa) fusion tag with mutations at three p…
    Available Since
    Aug. 31, 2009
    Availability
    Academic Institutions and Nonprofits only

  11. pEvolvR-mCherry-enCas9(H840A)-PolI5M

    Plasmid
    #249064
    Purpose
    Expresses nucleus-localized enCas9 (H840A) fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Article
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    enCas9(H840A)-PolI5M
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    249064-plasmid-map-sequence-id-495619

    Enlarge

  12. cBEST3

    Plasmid
    #234659
    Purpose
    Expresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertion
    Depositor
    Mingzi M. Zhang
    Article
    Lin et al Appl Environ Microbiol. 2025 Mar 10:e019
    Inserts
    spCas9 cytosine base editor
    Golden Gate compatible sgRNA insertion cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P3 and kasO17tss
    Available Since
    Nov. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    234659-plasmid-map-sequence-id-492747

    Enlarge

  13. pDestTol2CR-QUAS:NTR-mKate2

    Plasmid
    #241914
    Purpose
    Zebrafish transgenesis plasmid encoding nitroreductase-mKate2, driven by the QUAS enhancer, and including a cmlc2:mKate2 cardiac transgenesis marker
    Depositor
    Balázs Enyedi
    Article
    Fazekas et al J Cell Biol. 2026 Jan 5;225(1):e20250116
    Insert
    QUAS enhancer driving nitroreductase-mKate2
    Use
    Zebrafish tol2 transgenesis
    Tags
    nitroreductase fused with mKate2
    Promoter
    QUAS
    Available Since
    Oct. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    241914-plasmid-map-sequence-id-487159

    Enlarge

  15. pETDuet-solo-NHis6-NocO-D282A

    Plasmid
    #240407
    Purpose
    expresses N-His tagged NocO D282A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid
    Depositor
    Emily Balskus
    Article
    Wang et al Biochemistry. 2025 May 20;64(10):2173-21
    Insert
    nocO
    Tags
    His
    Expression
    Bacterial
    Mutation
    M1 of native sequence not included in insert, Asp…
    Promoter
    T7
    Available Since
    Aug. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    240407-plasmid-map-sequence-id-485005

    Enlarge

  16. pETDuet-solo-NHis6-NocO-H286A

    Plasmid
    #240409
    Purpose
    expresses N-His tagged NocO H286A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid
    Depositor
    Emily Balskus
    Article
    Wang et al Biochemistry. 2025 May 20;64(10):2173-21
    Insert
    nocO
    Tags
    His
    Expression
    Bacterial
    Mutation
    M1 of native sequence not included in insert, His…
    Promoter
    T7
    Available Since
    July 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    240409-plasmid-map-sequence-id-478125

    Enlarge

  17. pETDuet-solo-NHis6-NocO-H132A

    Plasmid
    #240404
    Purpose
    expresses N-His tagged NocO H132A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid
    Depositor
    Emily Balskus
    Article
    Wang et al Biochemistry. 2025 May 20;64(10):2173-21
    Insert
    nocO
    Tags
    His
    Expression
    Bacterial
    Mutation
    M1 of native sequence not included in insert, His…
    Promoter
    T7
    Available Since
    July 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    240404-plasmid-map-sequence-id-478123

    Enlarge

  18. pETDuet-solo-NHis6-NocO-E129A

    Plasmid
    #240403
    Purpose
    expresses N-His tagged NocO E129A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid
    Depositor
    Emily Balskus
    Article
    Wang et al Biochemistry. 2025 May 20;64(10):2173-21
    Insert
    nocO
    Tags
    His
    Expression
    Bacterial
    Mutation
    M1 of native sequence not included in insert, Glu…
    Promoter
    T7
    Available Since
    July 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    240403-plasmid-map-sequence-id-478122

    Enlarge

  19. pETDuet-solo-NHis6-NocO-Q128A

    Plasmid
    #240402
    Purpose
    expresses N-His tagged NocO Q128A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid
    Depositor
    Emily Balskus
    Article
    Wang et al Biochemistry. 2025 May 20;64(10):2173-21
    Insert
    nocO
    Tags
    His
    Expression
    Bacterial
    Mutation
    M1 of native sequence not included in insert, Glu…
    Promoter
    T7
    Available Since
    July 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    240402-plasmid-map-sequence-id-478121

    Enlarge

  20. pETDuet-solo-NHis6-NocO-E252A

    Plasmid
    #240405
    Purpose
    expresses N-His tagged NocO E252A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid
    Depositor
    Emily Balskus
    Article
    Wang et al Biochemistry. 2025 May 20;64(10):2173-21
    Insert
    nocO
    Tags
    His
    Expression
    Bacterial
    Mutation
    M1 of native sequence not included in insert, Glu…
    Promoter
    T7
    Available Since
    July 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    240405-plasmid-map-sequence-id-478119

    Enlarge

  21. pETDuet-solo-NHis6-NocO-E283A

    Plasmid
    #240408
    Purpose
    expresses N-His tagged NocO E283A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid
    Depositor
    Emily Balskus
    Article
    Wang et al Biochemistry. 2025 May 20;64(10):2173-21
    Insert
    nocO
    Tags
    His
    Expression
    Bacterial
    Mutation
    M1 of native sequence not included in insert, Glu…
    Promoter
    T7
    Available Since
    July 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    240408-plasmid-map-sequence-id-478118

    Enlarge

  22. pETDuet-solo-NHis6-NocO-H279A

    Plasmid
    #240406
    Purpose
    expresses N-His tagged NocO H279A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid
    Depositor
    Emily Balskus
    Article
    Wang et al Biochemistry. 2025 May 20;64(10):2173-21
    Insert
    nocO
    Tags
    His
    Expression
    Bacterial
    Mutation
    M1 of native sequence not included in insert, His…
    Promoter
    T7
    Available Since
    July 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    240406-plasmid-map-sequence-id-478117

    Enlarge

  23. pETDuet-solo-NHis6-NocO-E94A

    Plasmid
    #240401
    Purpose
    expresses N-His tagged NocO E94A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid
    Depositor
    Emily Balskus
    Article
    Wang et al Biochemistry. 2025 May 20;64(10):2173-21
    Insert
    nocO
    Tags
    His
    Expression
    Bacterial
    Mutation
    M1 of native sequence not included in insert, Glu…
    Promoter
    T7
    Available Since
    July 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    240401-plasmid-map-sequence-id-478116

    Enlarge

  24. pPMEL-Nluc-hbb

    Plasmid
    #239862
    Purpose
    Template for in vitro transcription of secreted nanoluciferase, with signal peptide from PMEL, flanked by the human haemoglobin beta 5' and 3' UTRs.
    Depositor
    Catherine Jopling
    Article
    Smart et al Mol Ther Nucleic Acids. 2025 Jun 24;36(3
    Insert
    Secreted nanoluciferase, with signal peptide from PMEL (gp100), flanked by human haemoglobin beta 5' and 3' UTRs
    Use
    Luciferase
    Promoter
    T7 (included as part of insert)
    Available Since
    July 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    239862-plasmid-map-sequence-id-477837

    Enlarge

  25. pDM097

    Plasmid
    #216819
    Purpose
    Aspergillus nidulans codon-adjusted mStayGold fluorescent protein, includes linker for N-terminal or internal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mStayGold
    Tags
    FLAG-(SGGS)x2-XTEN16-(GGGGS)x3 and c4-(GGGGS)x2-X…
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216819-plasmid-map-sequence-id-430144

    Enlarge

Showing: 931 - 960 of 1212 results