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Showing: 1551 - 1600 of 8550 results

  7. mCherry-Cb5-IRES-puromycin-pLVx-EF1a

    Plasmid
    #177434
    Purpose
    To express mCherry fused to Cb5 endoplasmic reticulum (ER) targeting sequence. Lentiviral vector used to make cell lines expressing this ER landmark.
    Depositor
    David Andrews
    Insert
    Cb5 tail anchor (Cyb5r4 Rat)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177434-plasmid-map-sequence-id-348465

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  12. LV_EF1a_Stat3(A662C,N664C,V667L)-P2A-Hygro_Barcode

    Plasmid
    #170254
    Purpose
    Barcoded lentiviral vector to express Stat3 (A662C, N664C, V667L) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq
    Depositor
    Prashant Mali
    Article
    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi
    Insert
    STAT3 (STAT3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    A662C, N664C, V667L
    Promoter
    EF1a
    Available since
    Sept. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    170254-plasmid-map-sequence-id-334374

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  17. pTRE-SOX17-P2A-Venus-rpEF1a-Zeo

    Plasmid
    #169174
    Purpose
    piggyBac transposon to generate a Tet inducible SOX17 expressing cell line. Tet-On inducible when paired with #169175
    Depositor
    Igor Slukvin
    Article
    Jung et al Cell Rep. 2021 Feb 16;34(7):108758. doi:
    Insert
    SOX17-P2A-Venus (SOX17 Synthetic, Human)
    Use
    Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pTREtight (tet-inducible promoter)
    Available since
    Jan. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    169174-plasmid-map-sequence-id-348368

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  24. pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry (PV2670)

    Plasmid
    #100061
    Purpose
    AAV expression of ChR1-VChR1 Chimera fused to mCherry driven by Ef1a promoter
    Depositor
    Karl Deisseroth
    Has Service
    AAV9
    Insert
    ChR1-VChR1 Chimera
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    E122T and E162T
    Promoter
    EF1a
    Available since
    Jan. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    100061-plasmid-map-sequence-id-194678

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  30. pAAV-EF1-fDIO-mNeon-WPRE-PolyA

    Plasmid
    #121546
    Purpose
    Super-bright fluorescence protein for tracking and visualization
    Depositor
    Matthias Prigge
    Insert
    cre-dependent mNeon
    Use
    AAV, Cre/Lox, and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    121546-plasmid-map-sequence-id-308125

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  33. pLenti-EF1a-Ly6G-P2A-PuroR-HA-miR30

    Plasmid
    #163359
    Purpose
    Lentiviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase, with expression of a Ly6G surface marker and a puromycin selection marker.
    Depositor
    Teunis Geijtenbeek, Louis Ates
    Article
    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.
    Insert
    Ly-6G (Ly6g Mouse)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    Jan. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    163359-plasmid-map-sequence-id-322106

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  36. pCDH-UBC-dendra 2LUC-EF1 Hygro

    Plasmid
    #129439
    Purpose
    express dendra2-luc chimera
    Depositor
    Oskar Laur
    Insert
    dendra2-luc
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    129439-plasmid-map-sequence-id-256444

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  40. pHAGE2-EF1a-E1MLS-2xMyc-Ku-IRES-Hygro

    Plasmid
    #234950
    Purpose
    Human codon optimized Ku (Mycobacterium) with N-terminal E1 MLS expressing plasmid
    Depositor
    Agnel Sfeir
    Article
    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.
    Insert
    human codon optimized Ku with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
    Use
    Lentiviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    234950-plasmid-map-sequence-id-467732

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  41. pAAV.T7-SP6-BC(p11-14)-Ef1s-CI-mRuby2-W3SL-T7

    Plasmid
    #231358
    Purpose
    Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses mRuby2 from Ef1s promoter.
    Depositor
    Viviana Gradinaru
    Article
    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103
    Insert
    T7-SP6-BC(p11-14)
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    231358-plasmid-map-sequence-id-459844

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  43. pRS415-TEF1pr-MFA-Igkappa-FAPoptim-STE3

    Plasmid
    #221114
    Purpose
    FAP tagged STE3 (N-terminally tagged, optimized for yeast expression) under the Tef1 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER
    Depositor
    Allyson O’Donnell
    Article
    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi
    Insert
    MFA-IgKappa-FAPoptim-STE3
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    221114-plasmid-map-sequence-id-442461

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  45. EF1a-FRT-MCS-WPRE-SV40 - GCH15

    Plasmid
    #230822
    Purpose
    AAV cloning vector for Flpo dependent expression under the EF1a promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230822-plasmid-map-sequence-id-457143

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  46. EF1a-Flex-MCS-WPRE-SV40 - GCH14

    Plasmid
    #230821
    Purpose
    AAV cloning vector for CRE dependent expression under the EF1a promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV and Cre/Lox; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230821-plasmid-map-sequence-id-457139

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  48. p5E-EFS (EF1a core) (JDW 1319)

    Plasmid
    #224474
    Purpose
    Gateway compatible 5' entry clone with human alpha 2 globin pause site (to prevent transcription readthrough) and EF1a core promoter. Lacks downstream intron for a lower-activity, compact promoter.
    Depositor
    Joshua Wythe
    Article
    Gillespie et al bioRxiv 2024.07.13.603267
    Insert
    EFS promoter
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    224474-plasmid-map-sequence-id-447208

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Showing: 1551 - 1600 of 8550 results