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Showing: 1551 - 1600 of 4899 results

  1. pAGT8163 (AATG_ttLbCas12a-i_TTCG)

    Plasmid
    #192453
    Purpose
    intronized ttLbCas12a for in planta expression
    Depositor
    Tom Schreiber
    Article
    Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4
    Insert
    temperature-tolerant LbCas12a with introns (BsaI-flanked Golden Gate module, MoClo L0)
    Use
    CRISPR and Synthetic Biology; Moclo compatible le…
    Tags
    Expression
    Mutation
    temperature-tolerant mutation (D156R)
    Promoter
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    192453-plasmid-map-sequence-id-379029

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  4. pNAD1

    Plasmid
    #154347
    Purpose
    pUC19 backbone with editing template for homologous recombination mediated deletion of Nitrate reductase gene of N. oceanica and selection based on zeocin resistance
    Depositor
    John van der Oost
    Article
    Naduthodi et al Biotechnol Biofuels. 2019 Mar 25;12:66.
    Insert
    ShBle
    Use
    Microalgae
    Tags
    Expression
    Bacterial
    Mutation
    none
    Promoter
    Violaxanthin Chlorophyll a Binding Protein Precur…
    Available since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    154347-plasmid-map-sequence-id-316607

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  8. loxP-G418-loxP-3xALFA-Halo-Rab11 HR

    Plasmid
    #229676
    Purpose
    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with Halotag and 3x ALFA tag. Contains a G418 resistance cassette for selection of edited cells
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence Rab11 locus (RAB11A Human)
    Use
    CRISPR
    Tags
    Halo, 3xALFA
    Expression
    Mutation
    Promoter
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229676-plasmid-map-sequence-id-457076

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  9. loxP-Puro-loxP-3xV5-SNAP-Rab11 HR

    Plasmid
    #229677
    Purpose
    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with SNAP tag and a 3x V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells.
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence Rab11 locus (RAB11A Human)
    Use
    CRISPR
    Tags
    SNAP, 3xV5
    Expression
    Mutation
    Promoter
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229677-plasmid-map-sequence-id-457129

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  10. AP1muA-mStayGold-polyA-Hygro HR

    Plasmid
    #229679
    Purpose
    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with monomeric StayGold. Contains a hygromycin resistance cassette for selection of edited cells
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence AP1M1 locus (AP1M1 Human)
    Use
    CRISPR
    Tags
    monomeric StayGold
    Expression
    Mutation
    Promoter
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229679-plasmid-map-sequence-id-457128

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  11. loxP-Hygro-loxP-HA-mStayGold-Rab11 HR

    Plasmid
    #229678
    Purpose
    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with monomeric StayGold and an HA epitope tag. Contains a hygromycin resistance cassette for selection of edited cells
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence Rab11 locus (RAB11A Human)
    Use
    CRISPR
    Tags
    monomeric StayGold, HA
    Expression
    Mutation
    Promoter
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229678-plasmid-map-sequence-id-457125

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  12. AP1muA-Halo-ALFA-polyA-G418 HR

    Plasmid
    #229681
    Purpose
    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with HaloTag and an ALFA tag. Contains a G418 resistance cassette for selection of edited cells.
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence AP1M1 locus (AP1M1 Human)
    Use
    CRISPR
    Tags
    Halo, ALFA
    Expression
    Mutation
    Promoter
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229681-plasmid-map-sequence-id-457127

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  13. AP1muA-SNAP-V5-polyA-Puro HR

    Plasmid
    #229680
    Purpose
    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with SNAP tag and a V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells.
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence AP1M1 locus (AP1M1 Human)
    Use
    CRISPR
    Tags
    SNAP, V5
    Expression
    Mutation
    Promoter
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229680-plasmid-map-sequence-id-457075

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  14. PX459-gR2A_Hinge

    Plasmid
    #124811
    Purpose
    Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a>Fab-srt-his plasmids to convert expression of hybridomas to Fab' fragments.
    Depositor
    Martijn Verdoes, Ferenc Scheeren
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    Cas9 – gRNA_R2A_hinge
    Use
    CRISPR
    Tags
    3XFLAG and GFP
    Expression
    Bacterial and Mammalian
    Mutation
    R166H in PuroR (please see depositors comment bel…
    Promoter
    pUC
    Available since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124811-plasmid-map-sequence-id-243700

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  19. AAV-pCbh-SpRY[C-term]-gRNAentry (CA20)

    Plasmid
    #197511
    Purpose
    Cbh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpRY and BsmBI entry cassette to clone SpCas9 gRNA spacer
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    AAV-[ITR]-pCbh-BPNLS-NpuC-SpRY[C-term]-BPNLS-gRNA[BsmBI]-pU6-[ITR]
    Use
    AAV and CRISPR
    Tags
    BPNLS and BPNLS-NpuC(intein)
    Expression
    Mutation
    C-terminal mutations of SpRY(L1111R/D1135L/S1136W…
    Promoter
    Cbh
    Available since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197511-plasmid-map-sequence-id-386647

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  23. pHybr_r2a>Fab-srt-his

    Plasmid
    #124810
    Purpose
    HDR template to knock-in sortag and histag in rat IgG2a heavy chain locus. Use in combination with PX459-gR2A_Hinge to convert rat IgG2a hybridoma to Fab' fragment producing cell lines.
    Depositor
    Martijn Verdoes, Ferenc Scheeren
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    rat IgG2a Hinge-G4S-Sortag-Histag
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial
    Mutation
    Promoter
    pUC
    Available since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124810-plasmid-map-sequence-id-243698

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  24. pminTolR4-R2

    Plasmid
    #67711
    Purpose
    mini Tol2 backbone with att4-attR2 cassette
    Depositor
    Nathan Lawson
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    67711-plasmid-map-sequence-id-117439

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  25. pILGFP1DFB

    Plasmid
    #185871
    Purpose
    A parental plasmid for in vivo gene amplification at RPL25 locus
    Depositor
    Claudia Vickers
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185871-plasmid-map-sequence-id-370815

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  26. pILGFP3AA5

    Plasmid
    #185875
    Purpose
    A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~47 copy)
    Depositor
    Claudia Vickers
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Aug. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    185875-plasmid-map-sequence-id-369502

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  27. pILGFP5EC4

    Plasmid
    #185880
    Purpose
    A plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (9 copy)
    Depositor
    Claudia Vickers
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185880-plasmid-map-sequence-id-370826

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  28. pILGFP3AG4

    Plasmid
    #185874
    Purpose
    A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~12 copy)
    Depositor
    Claudia Vickers
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185874-plasmid-map-sequence-id-370818

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  29. pILGFP5EF3

    Plasmid
    #185881
    Purpose
    A plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (13 copy)
    Depositor
    Claudia Vickers
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185881-plasmid-map-sequence-id-370828

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  30. pMT1004

    Plasmid
    #8613
    Depositor
    Robert Hartley
    Insert
    binase, barstar
    Use
    Tags
    Expression
    Bacterial
    Mutation
    as pMT1002
    Promoter
    Available since
    Jan. 17, 2007
    Availability
    Academic Institutions and Nonprofits only
    8613-plasmid-map-sequence-id-240677

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  31. pILGFP5EA4

    Plasmid
    #185879
    Purpose
    A plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (4 copy)
    Depositor
    Claudia Vickers
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185879-plasmid-map-sequence-id-370824

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  32. pILGFP4BG6

    Plasmid
    #185877
    Purpose
    A parental plasmid for in vivo gene amplification (HamAmp) at SEC23 locus
    Depositor
    Claudia Vickers
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185877-plasmid-map-sequence-id-370820

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  33. pILGFP3AE4

    Plasmid
    #185873
    Purpose
    A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~11 copy)
    Depositor
    Claudia Vickers
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185873-plasmid-map-sequence-id-370817

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  34. pILGFP5EG3

    Plasmid
    #185878
    Purpose
    A plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (1 copy reference)
    Depositor
    Claudia Vickers
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185878-plasmid-map-sequence-id-370821

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  35. pILGFP3A5C

    Plasmid
    #185872
    Purpose
    A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (1 copy reference)
    Depositor
    Claudia Vickers
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185872-plasmid-map-sequence-id-370816

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  36. pDmut06

    Plasmid
    #65945
    Purpose
    Theophylline riboswitch D mut derivatives 06
    Depositor
    Justin Gallivan
    Insert
    luciferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 3, 2015
    Availability
    Academic Institutions and Nonprofits only

  37. pDmut10

    Plasmid
    #65946
    Purpose
    Theophylline riboswitch D mut derivatives 10
    Depositor
    Justin Gallivan
    Insert
    luciferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 3, 2015
    Availability
    Academic Institutions and Nonprofits only

  38. NK690

    Plasmid
    #120849
    Purpose
    Reporter gene expression in S. rosetta
    Depositor
    Nicole King
    Insert
    Jumble-mWasabi
    Use
    Choanoflagellate expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    120849-plasmid-map-sequence-id-233698

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  39. pJKW0457

    Plasmid
    #107586
    Purpose
    AtU6-26 sgRNA
    Depositor
    Jing-Ke Weng
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    AtU6-26
    Available since
    April 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    107586-plasmid-map-sequence-id-208233

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  40. pILGFP3AG4ARSd

    Plasmid
    #185876
    Purpose
    A plasmid without an ARS sequence for in vivo gene amplification (HamAmp) at RPL25 locus (~12 copy)
    Depositor
    Claudia Vickers
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185876-plasmid-map-sequence-id-370819

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  41. pDA03

    Plasmid
    #65943
    Purpose
    Theophylline riboswitch D derivatives 03
    Depositor
    Justin Gallivan
    Insert
    luciferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Tac
    Available since
    Sept. 3, 2015
    Availability
    Academic Institutions and Nonprofits only

  42. pDA08

    Plasmid
    #65944
    Purpose
    Theophylline riboswitch D derivatives 08
    Depositor
    Justin Gallivan
    Insert
    luciferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Tac
    Available since
    Sept. 3, 2015
    Availability
    Academic Institutions and Nonprofits only

  43. pMDW139

    Plasmid
    #227336
    Purpose
    Template for in vitro Pol II transcription
    Depositor
    Michelle Wang
    Insert
    Gap DNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Plac
    Available since
    Nov. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    227336-plasmid-map-sequence-id-451681

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  44. NK676

    Plasmid
    #120852
    Purpose
    Reporter gene expression in S. rosetta
    Depositor
    Nicole King
    Insert
    pac-P2A-mTFP
    Use
    Choanoflagellate expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    120852-plasmid-map-sequence-id-233741

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  45. pfNL43-dE-EGFP

    Plasmid
    #36865
    Depositor
    Mario Chin
    Insert
    fNL43-dE-EGFP
    Use
    Lentiviral and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    inserted EGFP after 6350 nucleotide of original N…
    Promoter
    Available since
    Aug. 20, 2012
    Availability
    Academic Institutions and Nonprofits only
    36865-plasmid-map-sequence-id-46078

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  46. pGGC_RT_mScarlet

    Plasmid
    #136967
    Purpose
    Goldengate/Greengate CDS module, containing mScarlet
    Depositor
    Klaus Theres
    Insert
    mScarlet
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    136967-plasmid-map-sequence-id-269105

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  47. pGGD_RT_SRDX

    Plasmid
    #136976
    Purpose
    Goldengate/Greengate module EAR-repression domain
    Depositor
    Klaus Theres
    Insert
    SRDX
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    136976-plasmid-map-sequence-id-272093

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  48. pdcexADN

    Plasmid
    #227344
    Purpose
    Deletion of the AocexA gene in Aspergillus oryzae
    Depositor
    Jun-ichi Maruyama
    Insert
    Flanking regions of AocexA (AO090009000645)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    227344-plasmid-map-sequence-id-458569

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  49. pGGC_RT_Tq2CFP

    Plasmid
    #136969
    Purpose
    Goldengate/Greengate CDS module, containing Tq2CFP.
    Depositor
    Klaus Theres
    Insert
    Tq2CFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    136969-plasmid-map-sequence-id-270557

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  50. pGGD_RT_2NLS

    Plasmid
    #136977
    Purpose
    Goldengate/Greengate module nuclear localization signal
    Depositor
    Klaus Theres
    Insert
    SV40 NLS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    136977-plasmid-map-sequence-id-269089

    Enlarge

Showing: 1551 - 1600 of 4899 results