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pET28_Caspase-11_humanized-mutant

Plasmid

#214315

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Publication

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (Casp4 Mouse)

Tags

His-TEV

Expression

Bacterial

Mutation

N152K_KLS288YKT_KASIHS348TPRAKA

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pC0078 RESCUE

Plasmid

#130661

Purpose

pCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375S/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/

Depositor

Feng Zhang

Publication

Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dRanCas13b-ADAR2dd (RESCUE)

Expression

Mammalian

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pDF0582 huDisCas7-11 R1045-GGGS-R1122 U6-NT guide

Plasmid

#186991

Purpose

AAV transgene plasmid for Cas7-11-R1045-GGGS-R1122, with U6 promoter-driven expression of non-targeting crRNA guide

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Publication

Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

Insert

cas7-11-r1045-gggs-r1122, non-targeting crRNA guide

Use

AAV

Mutation

r1045-gggs-r1122 deletion

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pLV_TRET_Ngn2‐2A‐Ngn1

Plasmid

#61471

Purpose

3rd generation lentiviral vector; TetON promoter driving mouse Ngn2 and Ngn1

Depositor

Ron Weiss

Publication

Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

Inserts

Ngn2 (Neurog2 Mouse)
Neurog1 (Neurog1 Mouse)

Use

Lentiviral

Available since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pC0079 RESCUE-S

Plasmid

#130662

Purpose

pCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375A/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/S661T) V16S

Depositor

Feng Zhang

Publication

Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dRanCas13b-ADAR2dd

Expression

Mammalian

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pFAB3869

Plasmid

#47842

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 11 + BCD1 efficiency.

Depositor

Drew Endy

Publication

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 11 and BCD1

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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piCRg Entry

Plasmid

#58904

Purpose

Cas9/gRNA Entry expression plasmid

Depositor

Danwei Huangfu

Publication

Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hSpCas9

Expression

Mammalian

Available since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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p3XFLAG-CMV-14-WDR5

Plasmid

#59974

Purpose

Mammalian Expression of WDR5-FLAG

Depositor

Debu Chakravarti

Publication

Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

WDR5 (WDR5 Human)

Tags

FLAG

Expression

Mammalian

Available since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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EGFP-Rab11a-7

Plasmid

#56444

Purpose

Localization: Ras GTPase, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Publication

Fang et al Traffic. 2010 Jun;11(6):782-99. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rab11a (RAB11A Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

4 Articles

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pRSFDuet-v2 RsTAL At4CL

Plasmid

#126524

Purpose

expresses TAL (Rhodobacter sphaeroides) and 4CL (Arabidopsis thaliana paralog 1) for PYP chromophore biosynthesis

Depositor

Andrew Woolley

Publication

Brechun et al Biochemistry. 2019 Jun 11;58(23):2682-26

Inserts

tyrosine ammonia lyase
p-coumaroyl-CoA ligase (4CL1 Mustard Weed)

Tags

His

Expression

Bacterial

Promoter

T7

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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Watermelon backbone

Plasmid

#155258

Purpose

Can be used to generate a Watermelon library

Depositor

Joan Brugge, Aviv Regev

Publication

Oren et al Nature. 2021 Aug 11. pii: 10.1038/s41586

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pBABEhygro-Flag-ZO-2

Plasmid

#27473

Depositor

Marius Sudol

Publication

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
pEG BacMam C term 3C eGFP StrepII

Plasmid

#160686

Purpose

Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Publication

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Tags

3C eGFP StrepII

Expression

Mammalian

Promoter

CMV

Available since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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GFP-ING2(pHD)

Plasmid

#21589

Depositor

Junying Yuan

Publication

Gozani et al Cell. 2003 Jul 11. 114(1):99-111.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GFP-ING2(pHD) (Ing2 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

contains Ing2 plant homeodomain (PHD) amino acids…

Available since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pL2-G(TP)4TG

Plasmid

#137101

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Publication

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

G(TP)4TG

Use

Synthetic Biology

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV-Frankenbody-FLAG-mRuby2

Plasmid

#175294

Purpose

Track mature and nascent FLAG tagged proteins in live cells

Depositor

Tim Stasevich

Publication

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-FLAG frankenbody fused with mRuby2

Tags

mRuby2

Expression

Mammalian

Available since

Oct. 7, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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Venus-BimEL-pEGFP-C1

Plasmid

#166735

Purpose

Express Venus fused to the N-terminus of the Bcl-2 family protein, BimEL

Depositor

David Andrews

Publication

Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

BimEL (Bcl2l11 Mouse)

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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jk089_h12

Plasmid

#112151

Purpose

C. thermophilum DASH/Dam1 complex (construct used for cryo-EM structure determination)

Depositor

Stephen Harrison

Publication

Jenni et al Science. 2018 May 4;360(6388):552-558. d

Insert

C. thermophilum DASH/Dam1 complex

Tags

his tag on Spc34 and strep tag on Dad1

Available since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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MTK3_038

Plasmid

#123752

Purpose

Encodes R-Flinc-A, a cAMP reporter, as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

R-FlincA

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-ChrimsonR-GFP

Plasmid

#58806

Purpose

AAV expression of ChrimsonR-GFP under the Syn promoter

Depositor

Edward Boyden

Publication

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

K176R

Promoter

Syn

Available since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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