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pRSFDuet-v2 RsTAL At4CL

Plasmid

#126524

Purpose

expresses TAL (Rhodobacter sphaeroides) and 4CL (Arabidopsis thaliana paralog 1) for PYP chromophore biosynthesis

Depositor

Andrew Woolley

Publication

Brechun et al Biochemistry. 2019 Jun 11;58(23):2682-26

Inserts

tyrosine ammonia lyase
p-coumaroyl-CoA ligase (4CL1 Mustard Weed)

Tags

His

Expression

Bacterial

Promoter

T7

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pL2-G(TP)4TG

Plasmid

#137101

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Publication

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

G(TP)4TG

Use

Synthetic Biology

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pC0078 RESCUE

Plasmid

#130661

Purpose

pCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375S/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/

Depositor

Feng Zhang

Publication

Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

Insert

dRanCas13b-ADAR2dd (RESCUE)

Expression

Mammalian

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA3-HtrA2-FLAG

Plasmid

#15938

Depositor

L. Miguel Martins

Publication

Plun-Favreau et al Nat Cell Biol. 2007 Nov;9(11):1243-52. d

Insert

HtrA2 (HTRA2 Human)

Tags

FLAG

Expression

Mammalian

Available since

Nov. 5, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Venus-BimEL-pEGFP-C1

Plasmid

#166735

Purpose

Express Venus fused to the N-terminus of the Bcl-2 family protein, BimEL

Depositor

David Andrews

Publication

Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

Insert

BimEL (Bcl2l11 Mouse)

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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jk089_h12

Plasmid

#112151

Purpose

C. thermophilum DASH/Dam1 complex (construct used for cryo-EM structure determination)

Depositor

Stephen Harrison

Publication

Jenni et al Science. 2018 May 4;360(6388):552-558. d

Insert

C. thermophilum DASH/Dam1 complex

Tags

his tag on Spc34 and strep tag on Dad1

Available since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pLV_TRET_Ngn2‐2A‐Ngn1

Plasmid

#61471

Purpose

3rd generation lentiviral vector; TetON promoter driving mouse Ngn2 and Ngn1

Depositor

Ron Weiss

Publication

Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

Inserts

Ngn2 (Neurog2 Mouse)
Neurog1 (Neurog1 Mouse)

Use

Lentiviral

Available since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pC0079 RESCUE-S

Plasmid

#130662

Purpose

pCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375A/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/S661T) V16S

Depositor

Feng Zhang

Publication

Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

Insert

dRanCas13b-ADAR2dd

Expression

Mammalian

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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piCRg Entry

Plasmid

#58904

Purpose

Cas9/gRNA Entry expression plasmid

Depositor

Danwei Huangfu

Publication

Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

Insert

hSpCas9

Expression

Mammalian

Available since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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p3XFLAG-CMV-14-WDR5

Plasmid

#59974

Purpose

Mammalian Expression of WDR5-FLAG

Depositor

Debu Chakravarti

Publication

Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

Insert

WDR5 (WDR5 Human)

Tags

FLAG

Expression

Mammalian

Available since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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EGFP-Rab11a-7

Plasmid

#56444

Purpose

Localization: Ras GTPase, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Publication

Fang et al Traffic. 2010 Jun;11(6):782-99. doi: 10.

Insert

Rab11a (RAB11A Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

4 Articles

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pGEX-API5

Plasmid

#157660

Purpose

Expresses API5 in E.coli cell

Depositor

Byung Il Lee

Publication

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Apoptosis inhibitor 5 (API5 Human)

Tags

GST tag

Expression

Bacterial

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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Watermelon backbone

Plasmid

#155258

Purpose

Can be used to generate a Watermelon library

Depositor

Joan Brugge, Aviv Regev

Publication

Oren et al Nature. 2021 Aug 11. pii: 10.1038/s41586

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pEG BacMam C term 3C eGFP StrepII

Plasmid

#160686

Purpose

Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Publication

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Tags

3C eGFP StrepII

Expression

Mammalian

Promoter

CMV

Available since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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GFP-ING2(pHD)

Plasmid

#21589

Depositor

Junying Yuan

Publication

Gozani et al Cell. 2003 Jul 11. 114(1):99-111.

Insert

GFP-ING2(pHD) (Ing2 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

contains Ing2 plant homeodomain (PHD) amino acids…

Available since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pCL0952

Plasmid

#252419

Purpose

IPO11 tagged on the N-terminus with mCherry.

Depositor

Andrew Bowman

Publication

Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

Insert

IPO11 (IPO11 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

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pGEX-PAR6B-PDZ-1

Plasmid

#250124

Purpose

Bacterial expression of PAR6B PDZ domain 1

Depositor

Mark Bedford

Publication

Espejo et al J Biol Chem. 2017 Feb 10;292(6):2255-226

Insert

PAR6B (1/1) (Pard6b Mouse)

Expression

Bacterial

Mutation

None

Available since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pGEX-beta1-syntrophin-PDZ-1

Plasmid

#250071

Purpose

Bacterial expression of beta1-syntrophin PDZ domain 1

Depositor

Mark Bedford

Publication

Espejo et al J Biol Chem. 2017 Feb 10;292(6):2255-226

Insert

beta1-syntrophin (1/1) (Sntb1 Mouse)

Expression

Bacterial

Mutation

None

Available since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pJBM1

Plasmid

#225288

Purpose

E. coli- C. coli shuttle vector; KanR

Depositor

Robert Moore

Publication

McDonald et al mBio. 2024 Jul 29:e0110124. doi: 10.1128

Insert

Replication elements from pWestmill 11

Expression

Bacterial

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pST_APOE_HUMAN_V153A_G131S_11s (pBS0850)

Plasmid

#185281

Purpose

For the mammalian expression of the human protein APOE_HUMAN_V153A_G131S_11s. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOE_HUMAN_V153A_G131S_11s

Expression

Mammalian

Mutation

V153A, G131S

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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