We narrowed to 1,830 results for: FAST

Showing: 621 - 640 of 1830 results

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pTAG1

Plasmid

#176863

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Publication

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_1

Use

CRISPR

Mutation

WT

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTAG2E

Plasmid

#176865

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Publication

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_2E

Use

CRISPR

Mutation

WT

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac1_F253Y/F734-hDPP9-6His

Plasmid

#254906

Purpose

pFastBac1 vector for the recombinant overexpression in insect cells of the human DPP9 protein mutated at position 253 & 734.

Depositor

Ingrid De Meester

Publication

Beyens et al ChemMedChem. 2025 Mar 3;20(5):e202400700

Insert

Dipeptidyl peptidase 9 (DPP9 Human)

Tags

6His-tag

Expression

Insect

Mutation

Changed phenylalanine 253 to tyrosine and phenyla…

Promoter

Polyhedrin

Available since

June 16, 2026

Availability

Academic Institutions and Nonprofits only

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pFastBac1/CALR del52-His

Plasmid

#214795

Purpose

Baculovirus expression of human CALR del52-His

Depositor

Norio Komatsu

Publication

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human CALR del52-His (CALR Human)

Tags

His

Expression

Insect

Available since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac1/CALR ins5-His

Plasmid

#214796

Purpose

Baculovirus expression of human CALR ins5

Depositor

Norio Komatsu

Publication

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human CALR ins5 (CALR Human)

Tags

His

Expression

Insect

Available since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac1-His-GFP-RAD54L2

Plasmid

#247924

Purpose

Insect expression construct for N-terminal His-GFP-tagged RAD54L2

Depositor

Junjie Chen, Huimin Zhang

Publication

Zhang et al Sci Adv. 2023 Dec 8;9(49):eadi6681. doi:

Insert

RAD54L2 (RAD54L2 Human)

Tags

His-GFP

Expression

Insect

Available since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac1-ScVip1-186-1107

Plasmid

#238316

Purpose

ScVip1-186-1107 protein expression in insect cells

Depositor

Michael Hothorn

Publication

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-186-1107

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac SOX 318-320A

Plasmid

#192060

Purpose

Expression vector for SOX (ORF37) 318-320A

Depositor

Britt Glaunsinger

Publication

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

SOX (ORF37)

Expression

Insect

Mutation

318-320A

Promoter

Polyhedrin

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac1-NEDD4L_W2-W3-W4-HECT (349)

Plasmid

#187721

Purpose

Express GST-tagged NEDD4L W2-W3-W4-HECT (349) in insect cells

Depositor

Sandra Gabelli

Publication

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 349-975 (NEDD4L Human)

Tags

GST-HRV 3C site

Expression

Insect

Mutation

deletion of residues 1-348

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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PMP34-mApple-NFAST(low)

Plasmid

#214418

Purpose

Peroxisome localization of low-affinity NFAST fused to PMP34-Apple

Depositor

Chi-Lun Chang

Publication

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspA-N (SLC25A17 )

Tags

mApple

Expression

Mammalian

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pFASTbac-HisGST-PTPIP51(36-470)

Plasmid

#170531

Purpose

Expresses Cleavable HisGST-tagged PTPIP51 (36-470) in Sf9

Depositor

Byung Il Lee

Publication

Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

Insert

Protein tyrosine phosphatase interacting protein 51 (RMDN3 )

Tags

GST tag and His tag

Expression

Insect

Promoter

Polyhedrin

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac-LacI-LAP-HEC1

Plasmid

#114033

Purpose

Production of baculovirus with LacI-LAP-HEC1

Depositor

Geert Kops

Publication

Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

Insert

LacI-LAP-HEC1 (NDC80 Human)

Expression

Insect

Mutation

none

Promoter

CMV

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac(His10-Lckg2a,Y394F)

Plasmid

#177893

Purpose

Baculo expression of His10 tag fused to human LckG2A

Depositor

Ron Vale

Publication

Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

Insert

Lck (LCK Human)

Tags

10xHis-TEV

Expression

Insect

Mutation

G2A,Y394F

Promoter

Polyhedrin

Availability

Academic Institutions and Nonprofits only

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pB80-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11

Plasmid

#174644

Purpose

Optogenetic coupling of RAB11 to opto-kinesin to induce anterograde transport of recycling endosomes

Depositor

Lukas Kapitein

Publication

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)-P2A-iLID-mCherry-RAB11

Tags

Venus-SSPB and iLID-mCherry

Expression

Mammalian

Mutation

mmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…

Promoter

Chicken beta-actin

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCoofy27

Plasmid

#44003

Depositor

Sabine Suppmann

Publication

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

His6

Expression

Insect

Promoter

PPH

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pJC225

Plasmid

#176883

Purpose

MoClo Level1 plasmid, allows transition into Cermak, et al., 2017 GG cloning system

Depositor

Daniel Voytas

Publication

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Type

Empty backbone

Use

Cloning

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac Dual PNG-FLAG PLU-HIS

Plasmid

#113173

Purpose

Baculovirus expression of PNG and PLU

Depositor

Terry Orr-Weaver

Publication

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

PNG, PLU

Use

Baculovirus

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGEN-FastFUCCI-P2A-H2A-tdiRFP (JDW 1402)

Plasmid

#242591

Purpose

A CAGGS driven expression vector containing FastFUCCI to label cell cycle state followed by an H2A-tdiRFP for nuclear labeling.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)

Expression

Mammalian

Promoter

CAGGS

Available since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pFastBac1 human full-length CENP-E-mNeon-6xHis

Plasmid

#180484

Purpose

pFastBac1 human CENP-E-mNeon-His, containing a 3c protease cleavage site between mNeon and His tag. Codon optimized for expression in Sf9 cells

Depositor

Julie Welburn

Publication

Craske et al Open Biol. 2022 Mar;12(3):210389. doi: 1

Insert

CENP-E (CENPE human, Synthetic, Human)

Tags

mNeon-6xHis

Expression

Insect

Promoter

polyhedrin promoter

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus)

Plasmid

#79031

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Publication

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5alpha(African green monkey pygerythrus)

Tags

OneSTrEP-FLAG

Expression

Insect

Promoter

polyhedrin

Available since

March 3, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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