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pPIDCB

Plasmid

#169899

Purpose

Empty dox inducible vector with piggyBac, insulators, and a DTS

Depositor

Richard Schneider

Article

Chu et al Biol Open. 2020 Oct 6;9(10). pii: bio.05

Type

Empty backbone

Use

Avian expression

Tags

Expression

Mammalian

Mutation

Promoter

Dox inducible

Available since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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FRP1640_PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

Plasmid

#58440

Purpose

Cassette to replace yeast promoters with an inducible promoter containing 2 lexA boxes

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

Use

Tags

Expression

Yeast

Mutation

Promoter

PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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FRP1639_PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

Plasmid

#58439

Purpose

Cassette to replace yeast promoters with an inducible promoter containing 1 lexA box

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

Use

Tags

Expression

Yeast

Mutation

Promoter

PTEF-HygMX-TTEF-insul-(lexA-box)1-PminCYC1

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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Flag pLJM1 RagD 77L

Plasmid

#19317

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding D (RRAGD Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

S77L

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
pFvL118

Plasmid

#64752

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 epitope tag, Hygro selection

Depositor

Fred van Leeuwen

Article

Verzijlbergen et al Proc Natl Acad Sci U S A. 2010 Jan 5;107

Insert

RITE cassette

Use

Rite cassette

Tags

Expression

Mutation

Promoter

none

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pTW081

Plasmid

#64755

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, T7 to an HA epitope tag, Hygro selection

Depositor

Fred van Leeuwen

Article

Verzijlbergen et al Proc Natl Acad Sci U S A. 2010 Jan 5;107

Insert

RITE cassette

Use

Rite cassette

Tags

Expression

Mutation

Promoter

none

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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FRP1641_PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1

Plasmid

#58441

Purpose

Cassette to replace yeast promoters with an inducible promoter containing 3 lexA boxes

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1

Use

Tags

Expression

Yeast

Mutation

Promoter

PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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HA-VHL R167W-pRc/CMV

Plasmid

#22082

Depositor

William Kaelin

Article

Kibel et al Science. 1995 Sep 8. 269(5229):1444-6.

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Tags

HA-tag

Expression

Mammalian

Mutation

point mutant converting aa 167 from Arginine to T…

Promoter

Available since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pLIC.B3.TM0851

Plasmid

#11530

Depositor

Sung-Hou Kim

Article

Liu et al J Mol Biol. 2005 Jul 29. 350(5):987-96.

Insert

heat-inducible transcriptional repressor (TM0851 T. maritima)

Use

Tags

HisTag, TEV cleavable

Expression

Bacterial

Mutation

Promoter

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only

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Tip alpha (21-192)

Plasmid

#23157

Depositor

SeWon Suh

Article

Jang et al J Mol Biol. 2009 Sep 11. 392(1):191-7.

Insert

TNF-α-inducing protein

Use

Tags

His

Expression

Bacterial

Mutation

deleted amino acids 1-20

Promoter

Available since

April 16, 2010

Availability

Academic Institutions and Nonprofits only
pPROEX Hsp104 (773-908)

Plasmid

#1316

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 (773-908) (HSP104 Budding Yeast)

Use

Tags

6X His + TEV cleavage

Expression

Bacterial

Mutation

deletion of aa1-773

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pPROEX Hsp104 (361-548)

Plasmid

#1317

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 (361-548) (HSP104 Budding Yeast)

Use

Tags

6X His + TEV cleavage

Expression

Bacterial

Mutation

aa361-548 of Hsp104

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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Tip alpha (28-192)

Plasmid

#23158

Depositor

SeWon Suh

Article

Jang et al J Mol Biol. 2009 Sep 11. 392(1):191-7.

Insert

TNF-α-inducing protein

Use

Tags

His

Expression

Bacterial

Mutation

deleted amino acids 1-27

Promoter

Available since

April 16, 2010

Availability

Academic Institutions and Nonprofits only
pG0A0-1-1-PBAD-dCas9

Plasmid

#208815

Purpose

Expresses arabinose-inducible dCas9, codon-optimized for Salmonella

Depositor

Marc Erhardt

Article

Naseri et al Commun Biol. 2023 Oct 3;6(1):1005. doi:

Insert

Arabinose-inducible dCas9 that is codon optimized for expression in Salmonella

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Arabinsoe-inducible promoter

Available since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-LacR-TopBP1

Plasmid

#31317

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al J Biol Chem. 2011 Jun 3. 286(22):19229-3

Insert

TopBP1 (TOPBP1 Human)

Use

Tags

FLAG and LacR

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only

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pNat-AID*-9myc

Plasmid

#99523

Purpose

C-terminal AID*-9myc degron cassette with NatR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-116) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

9xMyc

Expression

Mutation

Promoter

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pSLIK DN RhoA

Plasmid

#84646

Purpose

Lentiviral expression of doxycycline-inducible dominant negative RhoA and co-expression of Venus

Depositor

Sanjay Kumar

Article

MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

Insert

RhoA (RHOA Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Mutation

Dominant Negative

Promoter

Available since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-ADAM10-HA

Plasmid

#65106

Purpose

Expression of HA tagged ADAM10 in mammalian cells

Depositor

Axel Ullrich

Article

Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

Insert

ADAM10 (ADAM10 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNAintron-XKR8-n3xFLAG

Plasmid

#215693

Purpose

Produces the XKR8 lipid scramblase with an n-terminal 3xFLAG tag

Depositor

Melinda Brindley

Article

Acciani et al J Virol. 2021 Sep 27;95(20):e0116521. do

Insert

XKR8-n3xFLAG (XKR8 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-ADAM17-HA

Plasmid

#65105

Purpose

Expression of HA tagged ADAM17 (TACE) in mammalian cells

Depositor

Axel Ullrich

Article

Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

Insert

ADAM17 (ADAM17 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

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