We narrowed to 10,449 results for: T7

1. pSm-Nuclease

   Plasmid

   #132431

   Purpose

   Expresses Serratia marcescens nuclease in E. coli using T7 promoter

   Depositor

   Mark Arbing

   Insert

   Serratia marcescens nuclease (codon optimized)
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. T7RNAP Cyslight A188C

   Plasmid

   #205083

   Purpose

   T7RNAP protein expression

   Depositor

   Smita Patel

   Insert

   T7RNAP Cyslight A188C (T7p07 Bacteriophage T7)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C125S, C347S, C510S, C515S, C530S, C723S N88C

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. T7RNAP Cyslight V597C

   Plasmid

   #205080

   Purpose

   T7RNAP protein expression

   Depositor

   Smita Patel

   Insert

   T7RNAP Cyslight V597C (T7p07 Bacteriophage T7)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C125S, C347S, C510S, C515S, C530S, C723S V597C

   Promoter

   Available since

   Aug. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. T7RNAP Cyslight V166C

   Plasmid

   #205079

   Purpose

   T7RNAP protein expression

   Depositor

   Smita Patel

   Insert

   T7RNAP Cyslight V166C (T7p07 Bacteriophage T7)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C125S, C347S, C510S, C515S, C530S, C723S V166C

   Promoter

   Available since

   Aug. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. C95m

   Plasmid

   #121001

   Purpose

   MoClo golden gate assembly CD part for T7 RNA polymerase (use with P18m). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   T7 RNA polymerase (Andy please check!)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. luxA+luxB/pRSETB Duet

   Plasmid

   #197581

   Purpose

   Expressing luxA and luxB under dual T7 promoter

   Depositor

   Takeharu Nagai

   Article

   Kaku et al Sci Rep. 2021 Jul 22;11(1):14994. doi: 1

   Insert

   LuxA LuxB
   

   Use

   Tags

   T7 tag and 6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTHSSd_18

   Plasmid

   #59953

   Purpose

   Quad split (at 61, 179, 601-SZ)

   Depositor

   Christopher Voigt

   Article

   Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

   Insert

   Four-fragment T7* RNA polymerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   PTac

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJin-278

   Plasmid

   #125714

   Purpose

   ABA-inducible split T7 GFP vector using RNAPN d5-19

   Depositor

   Bryan Dickinson

   Article

   Pu et al ACS Chem Biol. 2018 Feb 16;13(2):431-437

   Insert

   PT7-GFP mRNA expression, PYL-linker-T7 RNAPC, T7 RNAPN (d5-19)-linker-ABI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTHSSd_14

   Plasmid

   #59952

   Purpose

   Triple split (at 67, 601-SZ)

   Depositor

   Christopher Voigt

   Article

   Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

   Insert

   Three-fragment T7* RNA polymerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   PTac

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTNT-pT7-tetR-sfGFP

    Plasmid

    #140868

    Purpose

    T7 promoter driven synthesis of C-terminal fusion of sfGFP to TetR repressor (TetR-sfGFP)

    Depositor

    Neal Devaraj

    Article

    Niederholtmeyer et al Nat Commun. 2018 Nov 28;9(1):5027. doi:

    Insert

    TetR-sfGFP expressed with T7 promoter
    

    Use

    Synthetic Biology

    Tags

    sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJUMP19-T7pro_P

    Plasmid

    #127002

    Purpose

    Basic Part P- Promoter (non-constitutive); T7 promoter; recognized by T7 polymerase

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Insert

    Part T7 promoter_P
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZB604_pT7-sfGFP

    Plasmid

    #228510

    Purpose

    Bacterial Hybrid Reporter Plasmid (sfGFP), pT7-sfGFP

    Depositor

    Timothy Whitehead

    Article

    Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNoT7

    Plasmid

    #218168

    Purpose

    pET31b plasmid with short insert lacking T7 and ribosome binding sites as control

    Depositor

    Emily Weinert

    Article

    Chauhan et al ACS Cent Sci. 2022 Nov 23;8(11):1518-152

    Insert

    Short sequence, mutated RBS and T7 sites
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    NA - mutated T7

    Available since

    June 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKP2085

    Plasmid

    #167606

    Purpose

    Express ColicinB-V5-TEV-T7-mature MBP

    Depositor

    Kathleen Postle

    Insert

    cba-malE translational fusion
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    cba ORF fused to V5 tag, TEV protease site, T7 ta…

    Promoter

    Available since

    July 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET29b-H6_Streptavidin_sfGFP

    Plasmid

    #124296

    Purpose

    His6-tagged Streptavidin GFP fusion protein

    Depositor

    Mark Arbing

    Insert

    His6 tag - TEV protease site - T7 tag - Streptavidin (15-159) - superfolder GFP
    

    Use

    Tags

    His6 and T7 tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pH6-TEV-mCherry

    Plasmid

    #132430

    Purpose

    Expresses His6-tagged mCherry in E. coli using T7 promoter

    Depositor

    Mark Arbing

    Insert

    mCherry
    

    Use

    Tags

    N-terminal His6 tag with TEV protease site

    Expression

    Bacterial

    Mutation

    Codon optimized

    Promoter

    T7

    Available since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. Click editor (CE1) mRNA expression vector - T7-5'UTR-PCV2-nCas9-EcKlenow-3'UTR-polyA (CJT472)

    Plasmid

    #217808

    Purpose

    Expression vector for mRNA production of the click editor construct (CE1; PCV2-nCas9-EcKlenow)

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    5'UTR-PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(exo-)-BPNLS-3'UTR
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

    Promoter

    T7

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. Rich-pT7GG

    Plasmid

    #108443

    Purpose

    This plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pT7TS-rich. The vector is compatible for Golden-gate assembly

    Depositor

    Richard Bélanger

    Type

    Empty backbone

    Use

    Xenopus oocyte expression system

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. p15TV-L

    Plasmid

    #26093

    Purpose

    SGC Empty backbone for bacterial expression under T7 promoter. Uses infusion based cloning method.

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    6xHis and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    Sept. 6, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pYZ205

    Plasmid

    #104586

    Purpose

    Express SNAP-6His-CBD

    Depositor

    New England Biolabs, Yinhua Zhang

    Insert

    SNAP-6His-CBD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 4, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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