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pET28a-PHGDH

Plasmid

#74113

Purpose

Expression of N-terminal His-tagged

Depositor

Lewis Cantley

Article

Mullarky et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

Insert

PHGDH (PHGDH Human)

Use

Tags

His-tagged

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET14b-dabAB2

Plasmid

#133013

Purpose

pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a c-terminal strep tag fusion and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Inserts

dabB2
dabA2

Use

Tags

6xHis, StrepII tag, and sfGFP

Expression

Bacterial

Mutation

Promoter

t7

Available since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pET24a MDH_AQUAR

Plasmid

#204239

Purpose

Bacterial expression of A. arcticum malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

Depositor

Malate dehydrogenase CUREs Community (MCC)

Insert

MDH_AQUAR

Use

Tags

6X His

Expression

Bacterial

Mutation

Promoter

Lac

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pET28-2H12

Plasmid

#221186

Purpose

His6-tagged cdGreen expressed from T7 promoter (Internal Lab ID: UJ11308)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Insert

cdGreen

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pET24c(+)-EFTs

Plasmid

#31173

Depositor

Linda Spremulli

Article

Zhang et al FEBS Lett. 1996 Aug 12. 391(3):330-2.

Insert

E. coli elongation factor Ts

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
nifF in pET21b

Plasmid

#124520

Purpose

Azotobacter vinelandii flavodoxin II of Nitrogenase

Depositor

Douglas Rees

Article

Segal et al Protein Sci. 2017 Oct;26(10):1984-1993.

Insert

Flavodoxin II (nifF Azotobacter vinelandii)

Use

Tags

None

Expression

Bacterial

Mutation

Promoter

T7 / lac

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-SrtAdelta59

Plasmid

#51138

Purpose

expression plasmid for N-terminal his tagged S.aureus SrtA delta 59

Depositor

Hidde Ploegh

Article

Guimaraes et al Nat Protoc. 2013 Sep;8(9):1787-99. doi:

Insert

S.aureus SrtA delta 59

Use

Tags

6x His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only
pET28a_T7-ARG2

Plasmid

#106474

Purpose

Expresses acoustic reporter genes or gas vesicles (ARG2) in Escherichia coli

Depositor

Mikhail Shapiro

Article

Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

Insert

ARG2

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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pET24a MDH_9CREN

Plasmid

#204243

Purpose

Bacterial expression of I. islandicus malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

Depositor

Malate dehydrogenase CUREs Community (MCC)

Insert

MDH_9CREN

Use

Tags

6X His

Expression

Bacterial

Mutation

Promoter

Lac

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pETSUMO-TtAgo

Plasmid

#210191

Purpose

Bacterial expression of the His- and SUMO-tagged Argonaute protein of Thermus thermophilus

Depositor

Phillip Zamore

Article

Smith et al Nat Commun. 2019 Jan 17;10(1):272. doi:

Insert

TtAgo (TT_P0026 Thermus thermophilus)

Use

Tags

6x His tag and SUMO tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a MDHC_YEAST

Plasmid

#204232

Purpose

Bacterial expression of yeast cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).

Depositor

Malate dehydrogenase CUREs Community (MCC)

Insert

MDHC_YEAST (MDH2 Budding Yeast)

Use

Tags

6X His

Expression

Bacterial

Mutation

Promoter

Lac

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pET21a.Tat(48-57)-EGFP

Plasmid

#165040

Purpose

Expression of Tat(48-57) conjugated EGFP for protein purification (6XHis tag)

Depositor

Dokyun Na

Article

Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

Insert

EGFP

Use

Tags

6XHis and Tat(48-57)

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-AncMEcoGII250

Plasmid

#205025

Purpose

Expressing engineered M.EcoGII with higher thermostability

Depositor

Paola Laurino

Article

Ochiai et al Nucleic Acids Res. 2024 Oct 17:gkae887.

Insert

Ancestral M.EcoGII 250

Use

Tags

His6x tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pET22b-CPDSalI

Plasmid

#38251

Depositor

Aimee Shen, Matthew Bogyo

Article

Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

Type

Empty backbone

Use

Tags

6xHis and Vibrio cholerae MARTX toxin cysteine pr…

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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pET28a(+)- PC-MjMAT

Plasmid

#179372

Purpose

PC-MjMAT is an engineered methionine adenosyltransferase that catalyses the chemo-enzymatic generation of AdoMet analogs carrying photocaging groups.

Depositor

Andrea Rentmeister

Article

Peters et al Chembiochem. 2021 Oct 4. doi: 10.1002/cb

Insert

PC-MjMAT

Use

Tags

6xHis

Expression

Bacterial

Mutation

L147A, I351A

Promoter

T7

Available since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pET-30 Ubwt (2)

Plasmid

#26685

Depositor

Linda Hicke

Article

French et al J Biol Chem. 2009 May 1. 284(18):12071-9

Insert

Ubiquitin

Use

Tags

His and S-tag

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 17, 2010

Availability

Academic Institutions and Nonprofits only
pET-IG-mSA

Plasmid

#80706

Purpose

Expresses monomeric streptavidin (mSA) in bacterial cells

Depositor

Matthieu Sainlos

Article

Chamma et al Nat Commun. 2016 Mar 16;7:10773. doi: 10

Insert

Monomeric streptavidin

Use

Tags

10xHis tag and TEV site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pET28_Caspase-4_K356D

Plasmid

#214312

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (CASP4 Human)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

K356D

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-TRBP

Plasmid

#50351

Purpose

bacterial expression of TRBP

Depositor

Kumiko Ui-Tei

Article

Takahashi et al PLoS One. 2013 May 2;8(5):e63434. doi: 1

Insert

TRBP (TARBP2 Human)

Use

Tags

His and Myc

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pET3a-Yuh1

Plasmid

#18895

Depositor

Cecile Pickart

Article

Raasi et al Methods Mol Biol. 2005 . 301():47-55.

Insert

Ubiquitin C-terminal hydrolase I (YUH1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 4, 2008

Availability

Academic Institutions and Nonprofits only