We narrowed to 2,316 results for: pET28

1. pET28a-BCA-Pep-H

   Plasmid

   #185782

   Purpose

   Encodes enzyme bovine carbonic anhydrase linked to Pep-H self-assembling peptide via a GS-linker sequence

   Depositor

   Lizhong He

   Article

   Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

   Insert

   BCA-Pep-H
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28_6xHis-PARP1-A774L

   Plasmid

   #174796

   Purpose

   Bacterial expression of a hyperactive PARP1 mutant (A774L destabilizes the autoinhibitory HD subdomain)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-A774L (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A774L, V762A

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28_6xHis-PARP1-W318R

   Plasmid

   #174793

   Purpose

   Bacterial expression of an inactive PARP1 (W318R disrupts interdomain communication and HD subdomain unfolding)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-W318R (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   W318, V762A

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28_6xHis-PARP1-A762V

   Plasmid

   #174795

   Purpose

   Bacterial expression of wild type PARP1 mutant (A762V is reversion of the common V762A polymorphism)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-A762V (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A762V

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28b-trAtCCD8(pYL736)

   Plasmid

   #178287

   Purpose

   Expresses trAtCCD8 in E. coli. pET28b carrying t N-terminus 56 amino-acid truncated CCD8 from Arabidopsis

   Depositor

   Yanran Li

   Article

   Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

   Insert

   trAtCCD8
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-SpyTag002 DA-MBP

   Plasmid

   #102832

   Purpose

   Bacterial expression of unreactive version of SpyTag002 fused to maltose binding protein

   Depositor

   Mark Howarth

   Article

   Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

   Insert

   SpyTag002 DA-MBP
   

   Use

   Tags

   His6 and Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   Reactive aspartic acid has been mutated to an ala…

   Promoter

   T7

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a His6Flag2AUF1(p42)

   Plasmid

   #135941

   Purpose

   Expresses AUF1p42 in E. coli

   Depositor

   Yimon Aye

   Article

   Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

   Insert

   heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
   

   Use

   Tags

   Flag2 and His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28-At_PRORP1-His

   Plasmid

   #67869

   Purpose

   bacterial expression of PRORP1 (At2g32230), full coding sequence (mitoch./chloropl. form) + C-term. His tag

   Depositor

   Walter Rossmanith

   Article

   Pavlova et al Chembiochem. 2012 Oct 15;13(15):2270-6.

   Insert

   PRORP1 (PRORP1 Mustard Weed)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Residues 36-572 (see comments)

   Promoter

   T7

   Available since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-ClbN-NHis

   Plasmid

   #51497

   Purpose

   NRPS module containing CATE domains with N-terminal 6His tag

   Depositor

   Emily Balskus

   Article

   Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

   Insert

   ClbN
   

   Use

   Tags

   6x His tag and T7 tag

   Expression

   Bacterial

   Mutation

   contains aa30-1094 of NP_754347.1

   Promoter

   T7

   Available since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a-PTBP1-RRM12CCtoSS

    Plasmid

    #89155

    Purpose

    Recombinant expression of PTBP1-RRM12 C250S,C251S in E.coli

    Depositor

    Frédéric Allain

    Article

    Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

    Insert

    Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 1+2 mutant C250S, C251S (PTBP1 Human)
    

    Use

    Tags

    hexa His

    Expression

    Bacterial

    Mutation

    mutations: Cys 250 to Ser, Cys 251 to Ser

    Promoter

    T7

    Available since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28-p53 (1-73) (72R)

    Plasmid

    #62082

    Purpose

    Expression of human p53 (1-73) (72R) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    HIs

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-73; 72R

    Promoter

    T7

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pET28a-Hook3 aa 1-239

    Plasmid

    #74609

    Purpose

    Expresses Hook domain and first coiled coil of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-239 (HOOK3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28 mSmo-Cterm

    Plasmid

    #58703

    Purpose

    used for anti-mouse Smoothened antibody production (for affinity purification)

    Depositor

    Matthew Scott

    Article

    Rohatgi et al Science. 2007 Jul 20;317(5836):372-6.

    Insert

    Smo (Smo Mouse)
    

    Use

    Tags

    6X-HIS

    Expression

    Bacterial

    Mutation

    contains C terminus (aa 543-793)

    Promoter

    Available since

    Sept. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pET28b+R3(R112C/T180C)

    Plasmid

    #108195

    Purpose

    Expresses PdR-PCNA3(R112C/T180C) fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PdR-PCNA3(R112C/T180C) fusion protein
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C/T180C(PCNA3)

    Promoter

    T7 promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28b Me31B-3Myc

    Plasmid

    #113001

    Purpose

    For protein expression and purification of Myc-tagged full-length Drosophila me31B

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    full length me31B (me31B Fly)
    

    Use

    Tags

    Myc

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28b-sad1-C63A

    Plasmid

    #90302

    Purpose

    protein expression

    Depositor

    Christine Guthrie

    Article

    Hadjivassiliou et al RNA. 2014 May;20(5):656-69. doi: 10.1261

    Insert

    SAD1
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    C63A

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a His6Flag2AUF1(p40)

    Plasmid

    #135940

    Purpose

    Expresses AUF1p40 in E. coli

    Depositor

    Yimon Aye

    Article

    Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

    Insert

    heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
    

    Use

    Tags

    Flag2 and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28-At_PRORP3-His

    Plasmid

    #67871

    Purpose

    bacterial expression of PRORP3 (At4g21900), full coding sequence + C-term. His tag

    Depositor

    Walter Rossmanith

    Article

    Pavlova et al Chembiochem. 2012 Oct 15;13(15):2270-6.

    Insert

    PRORP3 (PRORP3 Mustard Weed)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28-his-3C_Clu3B6p

    Plasmid

    #100093

    Purpose

    expresses easily crystallizable engineered 14-3-3 sigma variant fused to the HSPB6 phosphorylatable peptide

    Depositor

    Nikolai Sluchanko

    Article

    Sluchanko et al Sci Rep. 2017 Sep 20;7(1):12014. doi: 10

    Insert

    14-3-3 sigma human (SFN Human)
    

    Use

    Tags

    HSPB6 peptide and His6

    Expression

    Bacterial

    Mutation

    75-77 residues changed to AAA to facilitate cryst…

    Promoter

    T7

    Available since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. 6his R.3-AR GUSpET28a+/pLysS

    Plasmid

    #61157

    Purpose

    N-terminal His tagged round 3 aldehyde-resistant GUS expression vector

    Depositor

    Andrew Ellington, Ichiro Matsumura

    Article

    Matsumura et al Nat Biotechnol. 1999 Jul;17(7):696-701.

    Insert

    GUSAR
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    N66D, D151N, A219V, I396T, T480A, Q498R, D508E an…

    Promoter

    T7

    Available since

    March 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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