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pHR-HOXD13-IDR-mCherry-CRY2-NLS

Plasmid

#145260

Purpose

Mammalian Expression of Nuclear Opto HOXD13 IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13wt-IDR (HOXA13 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-hSyn-EGFP-8X2C (LV GABA mAGNET)

Plasmid

#114212

Purpose

Lentiviral vector (control) that targets expression to GABAergic inhibitory neurons in rodent cortex

Depositor

Xue Han

Article

Keaveney et al Cell Rep. 2018 Jul 10;24(2):294-303. doi

Insert

Enhanced Green Fluorescent Protein

Use

Lentiviral and Mouse Targeting

Tags

None

Expression

Mammalian

Mutation

None

Promoter

human synapsin (hSyn)

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-RUNX2-IDR-mCherry-CRY2-NLS

Plasmid

#145267

Purpose

Mammalian Expression of Nuclear Opto RUNX2 IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

RUNX2wt-IDR (RUNX2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-SV40-mCherry-SHP1 (SHP2-cSH2)

Plasmid

#180822

Purpose

Expression of N-terminal mCherry tagged human SHP1 with its cSH2 replaced by SHP2-cSH2

Depositor

Enfu Hui

Article

Xu et al Elife. 2021 Nov 4;10. pii: 74276. doi: 1

Insert

SHP1 (SHP2-cSH2) (PTPN6, PTPN11 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SV40

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-mCherry-p2A-LIC1 Y101A, Y103A

Plasmid

#74605

Purpose

expresses LIC1 in mammalian cells; HA-tagged; point mutants that abrogate binding to dynein

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

full length human dynein light intermediate chain 1 Y101A, Y103A (DYNC1LI1 Human)

Use

Lentiviral

Tags

HA tag

Expression

Mammalian

Mutation

Y101A, Y103A

Promoter

SFFV

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pHR-HOXD13(+9A)-IDR-mCherry-CRY2-NLS

Plasmid

#145263

Purpose

Mammalian Expression of Nuclear Opto HOXD13(+9A) IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13(+9A)-IDR (HOXD13 Human)

Use

Tags

Expression

Mammalian

Mutation

A-repeat expanded

Promoter

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-FAS-NpHR3.0-eYFP-WPRE-pA

Plasmid

#37088

Purpose

Expresses NpHR3.0-EYFP in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic inhibition

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

NpHR3.0-EYFP

Use

AAV and Cre/Lox; Cre-off

Tags

EYFP

Expression

Mutation

Promoter

EF1a

Available since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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AktPH-mCherry-T2A-PHR-T2A-CIBNcaax

Plasmid

#173861

Purpose

Mammalian expression of Akt PH-mCherry-T2A-PHR-T2A-CIBNcaax (PIP3 marker and opto-control)

Depositor

Takao Nakata

Article

Kakumoto et al PLoS One. 2013 Aug 7;8(8):e70861. doi: 1

Insert

AktPH-mCherry-T2A-PHR-T2A-CIBNcaax

Use

Tags

C-terminal CAAX-box and mCherry

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pHR-mCherry-p2A-LIC1 C-terminal half

Plasmid

#74603

Purpose

expresses LIC1 in mammalian cells; aa 389-523, untagged but cells indicate expression with diffuse mCherry

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

full length human dynein light intermediate chain 1 amino acids 389-523 (DYNC1LI1 Human)

Use

Lentiviral

Tags

HA tag

Expression

Mammalian

Mutation

amino acids 389-523 of LIC1

Promoter

SFFV

Available since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

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PHR-LAT Y6F YFFF-mCitrine (XSB589)

Plasmid

#78517

Purpose

Lentiviral vector for expressing LAT-Y6F YFFF-mCitrine (0 Grb2 binding sites)

Depositor

Ron Vale

Article

Su et al Science. 2016 Apr 29;352(6285):595-9. do

Insert

LAT (LAT Human)

Use

Lentiviral

Tags

mCitrine

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-LAT-Y6F YYYY-mCitrine (XSB586)

Plasmid

#78515

Purpose

Lentiviral vector for expressing LAT-Y6F YYYY-mCitrine (3 Grb2 binding sites)

Depositor

Ron Vale

Article

Su et al Science. 2016 Apr 29;352(6285):595-9. do

Insert

LAT (LAT Human)

Use

Lentiviral

Tags

mCitrine

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pHR_5xUAS-ETV2-P2A-BFP-WPRE_PGK-HygR

Plasmid

#216664

Purpose

Expressed Gal4-inducible ETV2 and BFP, with hygromycin resistance

Depositor

Leonardo Morsut

Inserts

ETV2 (Etv2 Mouse)
BFP
HygromycinR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pHR-SNAP-CD86tm-eGFP-FKBP(f36v)

Plasmid

#200280

Purpose

Membrane protein fusion with extracellular SNAPtag and intracellular FKBP(f36v) dimerization domain and GFP

Depositor

Kristina Ganzinger

Article

Niederauer et al Nat Commun. 2023 Jul 19;14(1):4345. doi:

Insert

SNAP-tag-CD86tm-eGFP-FKBP(f36v)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-eGFP-CD86tm-HALOtag-FKBP(f36v)

Plasmid

#200281

Purpose

Membrane protein fusion with extracellular GFP and intracellular FKBP(f36v) dimerization domain and HALOtag

Depositor

Kristina Ganzinger

Article

Niederauer et al Nat Commun. 2023 Jul 19;14(1):4345. doi:

Insert

eGFP-CD86tm-HaloTag-FKBP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-TRE-MyoD-P2A-miRFP703_PGK-Puro

Plasmid

#216666

Purpose

Expresses tTa-inducible MyoD and miRFP703, with puromycin resistance

Depositor

Leonardo Morsut

Inserts

MyoD (Myod1 Mouse)
miRFP703
PuroR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-flag-Lag16-fibcon-iCAM1-tether

Plasmid

#205213

Purpose

This vector encodes of the synCAM tether (fibcon - iCAM1) and GFP nanobody LAG16

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM fibcon - iCAM1 Tether and Lag16 anti-GFP nanobody

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-PGK-eGFP-P2A-CRY2-VPR

Plasmid

#192610

Purpose

Lentiviral vector for constitutive expression of eGFP-P2A-CRY2-VPR.

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

eGFP-P2A-CRY2PHR-VPR

Use

Lentiviral and Synthetic Biology

Tags

eGFP

Expression

Mutation

Promoter

PGK

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pPHR-mCherry-RGG-GFP-RGG-mHP1alpha

Plasmid

#181893

Purpose

Soluble light-dependent effector protein (HP1alpha fused to the arginine/glycine-rich RGG domain of LAF-1) for optodroplet formation or recruitment to CIBN localizers

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

PHR-mCherry-RGG-GFP-RGG-mHP1alpha (Cbx5 Mustard Weed, Mouse)

Use

Tags

PHR-mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-SV40-BTLAecto&tmd-PD-1icd-mGFP

Plasmid

#180795

Purpose

Expression of human BTLA extracellular domain and transmembrane domain fused to PD-1 intracellular domain followed by mEGFP

Depositor

Enfu Hui

Article

Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

BTLAecto&tm-PD-1int (BTLA, PDCD1 Human)

Use

Lentiviral

Tags

mEGFP

Expression

Mutation

Promoter

SV40

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-HOXD13(-7A)-IDR-mCherry-CRY2-NLS

Plasmid

#145271

Purpose

Mammalian Expression of Nuclear Opto HOXD13(-7A) IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13(-7A)-IDR (HOXD13 Human)

Use

Tags

Expression

Mammalian

Mutation

A-repeat deleted

Promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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